蜜环菌胞外多糖的分离纯化及其性质研究

从蜜环菌发酵液提取粗多糖,经分离纯化得到多糖-A和多糖-B两个组分,快速纯化系统(FPLC)和电泳法鉴定各自为均一的组分。FPLC法测定A组分的分子量分别为2.0×105,苯酚-硫酸法测得其总糖含量为86.6%,考马斯亮蓝法测其蛋白含量为12.92%,硫酸-咔唑法测其酸性多糖含量为28%,红外光谱呈现多糖吸收特征峰,含有吡喃糖苷键。β-消去反应初步证明所提取的多糖-A存在O-糖肽键。     

巴戟天多糖的分离与纯化新方法

以巴戟天(Morinda officinalis How) 为原料,运用多种理化方法,提取纯化巴戟天粗多糖,经红外吸收光谱比较和鉴定确认提取物中有多糖特征吸收.采用苯酚-硫酸法测定多糖含量,以多糖的纯度和提取率为主要指标,对提取方法进行优化.结果表明,用乙醇-水提取法进行提取,用Sevag法除蛋白,氯化十六烷基吡啶(CPC)结合醇沉法进行纯化.正交实验结果表明提取纯化巴戟多糖最隹条件是乙醇浓度为85%,CPC浓度为0.4%,粗多糖浓度为10%的条件下,纯化多糖的纯度为89.7%,多糖的提取率为10.2%.     

沙棘叶水溶性多糖SJ21的分离纯化及组成分析

本文的目的是研究沙棘叶中的多糖组分.采用热水提取乙醇沉淀获得沙棘叶水溶性粗多糖的方法,用酸性乙醇和DEAE_SephdexA_25进行分离纯化,红外光谱鉴定多糖,纸层析及气相色谱分析其单糖组成.结果证明:纯度鉴定表明SJ21为纯多糖,分子量约为70KD.SJ21红外光谱中有典型的多糖和β型糖苷键吸收峰.纸层析及气相色谱分析其单糖组成为Xyl、Ara、Man、Glc、Gal,摩尔比为1.0∶2.1∶2.9∶5.3∶3.7.SJ21为首次从沙棘叶中提取获得.     

鸡腿菇子实体多糖分离纯化和光谱分析

从鸡腿菇子实体中提取纯化多糖,鉴定其纯度并测定其结构.利用水浴浸提、乙醇沉淀多糖、TCA法去除蛋白质等工艺提取粗多糖;将得到的粗多糖通过DEAE-52柱层析和Sephadex G-200柱层析进行分离纯化,收集得到4个糖组分Ccp-Ⅰ-A、Ccp-Ⅰ-B、Ccp-Ⅱ-C和Ccp-Ⅱ-D;采用紫外分光光度计、红外光谱仪对多糖结构进行初步的光谱分析.     

仙人掌多糖的分离纯化研究进展

仙人掌多糖具有明显的抗衰老、消炎、抗癌和降血糖等作用.文章综述了仙人掌多糖的多种提取和纯化及纯度鉴定的方法,以期为仙人掌多糖的进一步研究开发提供参考.     

佛手氨基酸及多糖含量的测定

为了测定佛手中氨基酸及多糖的含量，采用微量凯氏定氮法测定粗蛋白，氨基酸自动分析仪测定氨基酸；用柱层析法分离、纯化多糖，电泳鉴定其纯度，苯酚—硫酸法比色测定多糖的含量。结果表明佛手中ω(粗蛋白)为9.375％，ω(氨基酸)为8．334％，含16种氨基酸，其中人体必需的氨基酸有6种；佛手水提取液经分离、纯化，得到3种多糖，经电泳鉴定为单一多糖，命名为JFS-Ⅰ、JFS-Ⅱ和JFS-Ⅲ，苯酚—硫酸比色法测得3种多糖的质量分数分别为41．2％、9．8％和21．4％。说明佛手具有较为丰富的氨基酸和多糖，提示有较高的营养价值和药用价值。     

沙蒿多糖分离纯化和理化分析

利用水提醇沉法从白沙蒿子中提取多糖;Sevage 木瓜蛋白酶法脱蛋白,ultrahydrogelTM500(7.8×300 mm)凝胶柱层析进行分离纯化;琼脂糖电泳进行纯度检查;苯酚-硫酸法测定总糖含量;UV法及IR法检测多糖性质;自动旋光仪测定旋光度;离子色谱鉴定多糖的单糖组分.结果表明,沙蒿多糖为均一组分,纯度为96.5%,比旋光度为 90°,还原性多糖.经离子色谱分析,沙蒿多糖由阿拉伯糖、木糖、来苏糖、甘露糖、葡萄糖、半乳糖组成,且物质的量之比为1∶4.98∶1.69∶27.86∶3.76∶13.92.     

灵芝菌发酵产物中多糖组分的分离与性质研究

从灵芝的发酵产物中提取到水溶性多糖，经乙醇分级，重结晶纯化得到多糖组分ＧＰ，由凝胶过滤，琼脂糖电泳证明为化学均一性多糖，分子量约为２．９×１０＾４，将多糖组分ＧＰ水解后，用纸导析分析鉴定出由三种单糖组成：葡萄糖，木糖和甘露糖，此外，对纯多糖组分还进行了比旋度，熔点，紫外与红外光谱的性质测定。     

植物多糖研究概况

植物多糖由于它们独特的功能和低毒性,作为新药发展的方向具有广阔的应用前景.综述了植物多糖的提取分离、纯化、表征鉴定的方法和研究进展,讨论了其在医药领域潜在应用价值.并就实验室对芦荟多糖的研究作了简要的概述.     

长白山林蛙输卵管糖蛋白的分离鉴定

以长白山林蛙为研究对象, 对其输卵管糖蛋白进行提取分离及鉴定. 长白山林蛙输卵管冲洗液经饱和硫酸铵沉淀, DEAE-Cellulose 52离子交换柱, Sephadex G-100凝胶柱层析纯化, 得到蛋白质和糖的重合峰, 经透析、 冷冻干燥得长白山林蛙输卵管糖蛋白纯品OGP-Ⅰ. SDS PAGE电泳实验结果表明, OGP-Ⅰ是分子量为116 000的均一带. 对其分别进行蛋白和糖染色, 同一位置都出现单一条带; 紫外全扫描OGP-Ⅰ有多糖特征峰和蛋白吸收峰, 表明OGP-Ⅰ是糖蛋白纯品.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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Brief Introduction to “Journal of Jilin University( Science Edition) ”

<正>"Journal of Jilin University(Science Edition)"is a comprehensive academic journal in the fields of science sponsored by Jilin University and administrated by the Ministry of Education of the Peoples Republic of China.The journal started publication in 1955.The original name at starting publication was"Journal of Natural Science of Northeast People University",which was changed into"Acta Scientiarum Naturalium Universitatis Jilinensis"in 1958 owing to the name change of the university.The present journal name has