金边瑞香不定芽的增殖培养

通过2次单因子试验,研究了BA（0．5mg／L～5mg／L）和NAA（0．1mg／L～1mg／L）在金边瑞香不定芽增殖培养中的作用。BA在诱导产生不定芽的过程中起着主导作用。BA浓度在0．5mg／L时,培养6周产生的不定芽总数不多,但有效芽（〉1cm）相对较多,平均3．9个,随着BA使用浓度增加,产生的不定芽总数增多,但有效芽数量减少,BA浓度在3mg／L以上时,平均只有1个有效芽。NAA浓度在0．2mg／L-0．5mg／L范围内,平均有效芽数在3．8～3．6个,浓度增加到1mg／L时,有效芽数减少到平均1．8个。试验确定合适的不定芽增殖培养基为MS＋BA0．5mg／L＋NAA0．2mg／L。     

花生体细胞胚和丛生芽的诱导及扩繁培养

以花生成熟胚培养成的无菌苗叶节为外植体 ,在高浓度 6-BA ( 3 0 mg/ L) MS培养基中 ,光强 2 0 0 0Lx,( 2 5± 3 )℃和 1 4 / 1 0光暗比条件下培养 ,50天后全部诱导分化出整齐一致的丛生芽 ,每个外植体平均产生 8.2 5个丛生芽 ;切下带少许丛生芽的组织块转入含 6-BA ( 3 0 mg/ L) ,LH ( 40 0 mg/ L)和 YE ( 0 .0 1 % )的 MS培养基中 ,可诱导出新的丛生芽 ,诱导率达 98.70 % ,平均出芽 1 3 .86个 .将花生成熟胚轴转接于含NAA ( 1 mg/ L)和 6-BA ( 3 mg/ L) MS培养基中 ,2 0～ 3 0天后 ,诱导出大量正常的体细胞胚 ,诱导率达 95.7% ,将胚切成小块转入含 2 ,4 -D ( 2 0 mg/ L) ,6-BA ( 3 mg/ L )和 LH ( 2 0 0 m g/ L) MS培养基中 ,经 1 5天培养 ,诱导出白色致密的胚性愈伤组织 .     

两级厌氧工艺处理茶多酚废水的工业化研究

研究了工业规模两级 UASB反应器在不同温度、不同运行方式下对茶多酚废水的处理效能。结果表明 ,将一级高温 UASB与一级中温 UASB反应器串联 ,系统出水水质最佳。当系统 ρ(进水 COD) 2 1 0 0 7～ 2 5 772 mg/L,有机负荷 (以 COD计 ) 8.2 kg/( m3·d)左右 ,系统 ρ(出水 COD)可降低到 1 5 6 8mg/L以下。     

肇庆七星岩原尾蜥虎生态学研究

报道了原尾蜥虎 (HemidactylusbowringiiGiray)的生态学观察研究。成体平均全长10 7 0 4mm ,尾长为体长的 1 11倍 ,尾再生能力强。 5～ 10月为活动盛期 ,5～ 6月为繁殖高峰期 ,此期雌雄易鉴别。秋季种群结构明显 ,个体最多。 15℃以下完全入眠 ,2 0℃以下陆续出蛰。雌雄性腺发育具有较明显的季节变化 ,雄性睾丸 5月为最大值 ,重量 2 2 5mg ,其长径为 7 2 8(6 8～ 7 5 )mm ,短径为 4 2 9(4 0～ 4 5 )mm。 8月降为最低值 ,重量 3 74mg ,其长径为 4 6 2 (4 5～ 5 0 )mm ,短径为 1 2 2 (1 1～ 1 4)mm。一年一次性产卵 2枚 ,卵重 0 2 941g ,其长径为9 5 8(8 45～ 11 4)mm ,短径为 7 91(7 70～ 8 41)mm ,孵化期 42～ 46d ,6月中旬出现当年幼体     

桑树遗传转化技术中抗生素的浓度优化研究

调查了抗生素对根癌农杆菌介导的桑品种“新一之濑”遗传转化不同培养阶段外植体生长、分化或生根的影响及其对农杆菌的抑制效果 ,并确定 :在叶盘 (或茎尖 )转化筛选培养阶段 ,Kan (卡那霉素 )和 Cab (羧苄青霉素 )适宜浓度分别为 3 0～ 40 mg/ L和 2 0 0～40 0 mg/ L (或 6 0～ 80 mg/ L和 2 0 0～ 6 0 0 mg/ L ) ,在抗性芽继代 (或生根 )培养阶段 ,Kan和Cab分别为 40～ 5 0 mg/ L和 1 0 0～ 2 0 0 mg/ L (或 5～ 1 0 mg/ L和 5 0～ 1 0 0 mg/ L) .Cef(头孢霉素 )对桑树各种外植体的影响效果与 Cab相似 ,但两者的抑菌效果因农杆菌菌株、外植体类型以及培养阶段的不同而存在差异 ,用含 Kan、Rif(利福平 )和 Str(链霉素 )的 LB培养基浸渍叶盘则出现分化率下降、褐化死亡较严重的现象 .     

小麦叶基切段愈伤组织的诱导和植株再生

从3～5d龄小麦籽苗幼叶基部切段诱导出大量愈伤组织。诱导可再生的愈伤组织的培养基为MS 1．5mg／L 2，4-D 0．2mg／L，激动素(KT) 500mg／L，水解酪蛋白(CH) 120mg／L，天冬酰胺 100mg／L，肌醇 3％蔗糖 0．7％琼脂粉。当愈伤组织转移到生长调节物质改变为2mg／L KT，1mg／L NAA和0．1mg／L 2，4-D的MS培养基上培养后，出现了苗的分化。当分化苗在无机盐分减半的MS培养基上培养后，产生了根。愈伤组织的形成和植株再生的频率与外植体的位置和籽苗的发育阶段密切相关，3～4d龄苗叶基1cm处的切段效果最好。叶基愈伤组织的分化能力可保持3～4个月。     

敌百虫对九孔鲍稚鲍和真猛水蚤的毒性作用

不同温度下 ,敌百虫对稚鲍的毒性作用不同 ,在水温 2 1～ 2 2℃下 ,2 4h、 4 8h、72h、96h的半致死浓度分别为 182mg/L、 4 6mg/L、10 6mg/L ,6 6mg/L ,安全浓度为 4 6mg/L ;水温 2 6～ 2 7℃ ,在相同时间内的半致死浓度分别降至 12 9mg/L、 11 1mg/L、 8 2 5mg/L和5 1mg/L ,安全浓度为 1 11mg/L ;敌百虫对真猛水蚤的毒性也是随着水温升高而增强 ,水温2 1～ 2 2℃ ,2 4h、 4 8h、 72h的半致死浓度分别为 2 7× 10 - 1mg/L、 3 5× 10 - 2 mg/L、 2 0×10 - 2 mg/L ,安全浓度为 3 5× 10 - 2 mg/L ;当水温升至 2 6～ 2 7℃时 ,半致死浓度分别为 8 3× 10 - 2mg/L、8 3× 10 - 3mg/L、3 6× 10 - 3mg/L ,安全浓度为 8 3× 10 - 4mg/L 敌百虫对稚鲍的亚急性作用结果是 :在 0 4～ 2 0mg/L下生活 2 0d ,稚鲍的壳长、体重、摄食及存活率随其浓度的增大而降低 本文对九孔鲍工厂化育苗中如何正确使用敌百虫防治底栖桡足类提供了科学依据     

杜仲组织离体培养研究

以杜仲(EncommiaulmoidesOliver)当年生枝条的叶片、带芽茎段为外植体,基本培养基为MS,附加不同浓度的BA、KT和NAA.最佳芽诱导培养基为:MS KT1 0mg/L NAA0 1～0 5mg/L;MS BA2 0mg/L NAA0 1～0 5mg/L,最佳芽丛增殖培养基为:MS KT2 0mg/L NAA0 1～0 2mg/L;MS BA1 0～2 0mg/L NAA0 1～0 2mg/L;MS KT2 0mg/L IBA0 1～0 2mg/L;MS BA1 0～2 0mg/L IBA0 1～0 2mg/L,单芽增殖培养基:MS KT1 0mg/L NAA0 1mg/L,生根培养基:13MS KT0 1mg/L NAA0 5mg/L.     

不同取代度的甲壳素磷酸酯钾对番茄种子萌发的作用

用甲壳素合成1^#～7^#甲壳素磷酸酯和甲壳素磷酸酯钾，分别测定1^#～7^#甲壳素磷酸酯的取代度、将甲壳素磷酸酯钾配成O．5mg／L，2mg／L，5mg／L．10mg／L，20mg／L溶液，以蒸馏水为对照，进行番茄(Lycopeysicon esculentum)种子的浸种发芽实验。浸种46．5～76．5h；培养温度29．5C；每只培养皿50粒，每种浓度重复3次。结果表明：6^#、7^#甲壳素磷酸酯钾对番茄种子不具有促进作用；而1^#～5^#甲壳素磷酸酯钾对番茄种子有不同程度的促进作用，其质量浓度以0．5mg/OL～20mg／L为最佳，其中1^#、2^#、3^#、4^#、5^#甲壳素磷酸酯钾对番茄种子的发芽率分别比对照组增长6．7％、17．4％、16．7％、6．0％、16．0％。     

反相高效液相色谱法同时测定4种苯二氮Chuo类药物

提出了一种反相高效液相色谱法同时测定4种苯二氮Chuo类药物的分析方法。考察了4种苯二氮Chuo类药物的保留值与流动相组成、流速、pH及柱温等色谱条件的关系，优化了色谱条件．确定了以ODS Hypersil为色谱柱，甲醇-水(5l：49；V／V，用氨水调pH7．8)为流动相，流速为0．8mL／min，柱温为45℃，检测波长为220nm的最佳色谱条件,甲苯作为内标物，以内标法峰面积定量,硝西泮，地西泮，三唑仑和艾司唑仑线性范围分别是0．1mg／L～320mg／L，0．2mg／L～320mg／L，0．1mg／L～320mg／L和0．08mg／L～320mg／L．检测限分别为0．1mg／L，0．2mg／L，0．1mg／L和0．08mg／L,该方法用于尿液中的苯二氮革类药物的测定,其回收率为97．0％～108．7％，标准偏差为1．7％～3．0％(n=7),该方法简单、快速、精确、灵敏、重复性好。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that