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1.
Influence of pulsing electromagnetic field on the frequency of sister-chromatid exchanges in cultured mammalian cells 总被引:1,自引:0,他引:1
Exposures of Chinese hamster cells to pulsing electromagnetic field (PEMF) with 0.18-2.5 mT did not influence the baseline frequency of sister-chromatid exchanges (SCE). The results suggest that PEMF with the magnetic intensity examined does not interfere with DNA replication nor produce DNA lesions, thereby leading to an increased frequency of SCE. 相似文献
2.
We studied mice from five strains (BALB/c, C3H/HeSnJ, C57BL/6J, Csb and 129/ReJ) at two ages (young, 10 +/- 1 weeks; and old, 67 +/- 3 weeks) for the induction of sister chromatid exchanges (SCEs) in vivo by methyl nitrosourea (MNU). The SCE frequency is genotype-specific. The F1 phenotype resembles the 'low' responding parent. SCE induction is significantly lower in the older animals of each strain than their younger counterparts, and the reduction of SCE/cell with old age is strain-specific. A general explanation for these results must include strain differences in relative mutagenic sensitivity, genotype-specific pattern of reduction in DNA repair and other such factors affecting SCE formation, with old age. 相似文献
3.
Summary We studied mice from five strains (BALB/c, C3H/HeSnJ, C57BL/6J, Csb and 129/ReJ) at two ages (young, 10±1 weeks; and old, 67±3 weeks) for the induction of sister chromatid exchanges (SCEs) in vivo by methyl nitrosourea (MNU). The SCE frequency is genotype-specific. The F1 phenotype resembles the low responding parent. SCE induction is significantly lower in the older animals of each strain than their younger counterparts, and the reduction of SCE/cell with old age is strain-specific. A general explanation for these results must include strain differences in relative mutagenic sensitivity, genotype-specific pattern of reduction in DNA repair and other such factors affecting SCE formation, with old age. 相似文献
4.
J. R. Lazutka V. Dedonytè R. K. Lekevičius 《Cellular and molecular life sciences : CMLS》1992,48(5):508-512
The effects of alcohol consumption, cigarette smoking and age on sister chromatid exchangen (SCE) frequency in human lymphocytes were assessed by means of multiple linear regression. An increase in SCE rates was associated with alcohol consumption (p=0.0001), smoking (p=0.0231), and, to a small extent (p=0.057), age. These three confounding factors explain 48% of the inter-personal variation in SCE rates among subjects studied.We are grateful to Dr D. Krapavickaité for performing, the statistical analysis, and to Mrs V. Navickiené for technical assistance. We also wish to thank Dr B. Lambert (Stockholm, Sweden) for his valuable suggestions. 相似文献
5.
The effects of alcohol consumption, cigarette smoking and age on sister chromatid exchange (SCE) frequency in human lymphocytes were assessed by means of multiple linear regression. An increase in SCE rates was associated with alcohol consumption (p = 0.0001), smoking (p = 0.0231), and, to a small extent (p = 0.057), age. These three confounding factors explain 48% of the inter-personal variation in SCE rates among subjects studied. 相似文献
6.
D C Mourelatos 《Experientia》1979,35(6):822-824
The SCE frequency induced by Thiotepa and the effect of this antineoplastic drug in combination with caffeine have been studied in cultures of human peripheral blood. Caffeine was found to enchance SCE and breakage frequencies induced by Thiotepa in human lymphocytes. 相似文献
7.
The in vivo SCE test was used to demonstrate significant inhibition of NMU bone marrow genotoxicity by pretreatment of Chinese hamsters with n-alkanols. Our findings exclude a loss of intracellular DNA alkylation potential through a competitive direct reaction of NMU with the weakly nucleophilic polar end of the n-alkanols, but not through methylations of nucleophilic membrane sites possibly liberated by structural modifications which the membrane-active amphiphilics induce. 相似文献
8.
D. C. Mourelatos 《Cellular and molecular life sciences : CMLS》1979,35(6):822-824
Summary The SCE frequency induced by Thiotepa and the effect of this antineoplastic drug in combination with caffeine have been studied in cultures of human peripheral blood. Caffeine was found to enhance SCE and breakage frequencies induced by Thiotepa in human lymphocytes.Acknowledgment. I am deeply grateful to Dr M.J.W. Faed, for stimulating discussions, guidance and constructive criticism. This paper is a part of my Ph.D. thesis submitted to Dundee University. 相似文献
9.
M. Bullejos M. Burgos R. Jiménez A. Sánchez R. Díaz de la Guardia 《Cellular and molecular life sciences : CMLS》1996,52(6):511-515
We used the X chromosomes ofMicrotus cabrerae as a model to analyze the distribution of sister chromatid exchanges (SCEs) on different types of chromatin, because of the marked heterogeneity of the heterochromatin in the entire short arm and a portion of the long arm of this chromosome. Computer-simulated distributions, according to an algorithm that makes it possible to modify the distribution on the basis of any possible hypothesis, were compared with real distributions by log-linear models. We found that the frequency of SCEs in different types of heterochromatin was higher than that expected for a random distribution, and located an SCE hot-spot at the junction between euchromatin and heterochromatin. The possible relationship between the distribution of SCEs and base composition or chromatin accessibility are discussed. 相似文献
10.
H. W. Renner 《Cellular and molecular life sciences : CMLS》1979,35(10):1364-1365
Summary A mutagenic effect of saccharin in Chinese hamsters, using the in vivo SCE test, was observed when massive overdoses were administered; cyclamate was not mutagenic. 相似文献
11.
H W Renner 《Experientia》1979,35(10):1364-1365
A mutagenic effect of saccharin in Chinese hamsters, using the in vivo SCE test, was observed when massive overdoses were administered; cyclamate was not mutagenic. 相似文献
12.
Summary Although requirements for transformation inBranhamella catarrhalis are quite complex, DNA synthesis does not appear to be one of these needs, as indicated by the inability of nalidixic acid to interefere with transformation. Exogeneous sources of energy, such as cAMP and cGMP, also failed to enhance frequency, suggesting cells may actively engage in energy production to achieve uptake of DNA, or lack a transport mechanism for these compounds. 相似文献
13.
F. Cortés 《Cellular and molecular life sciences : CMLS》1980,36(11):1290-1291
Summary Distribution of SCE in C band and non-C band regions ofAllium cepa chromosomes. 相似文献
14.
T S Kochhar 《Experientia》1988,44(1):62-63
The influence of steroid hormones on the induction of sister-chromatid exchange (SCE) in cultured CHO cells was studied. It was observed that estradiol-17 beta, estriol, estrone and ethynyl estradiol treatments enhanced SCE rates compared to the controls. Overall, these compounds produced a dose response effect. The importance of a detailed study on the long-term genetic effects of steroids on mammalian cells is emphasized. 相似文献
15.
T. S. Kochhar 《Cellular and molecular life sciences : CMLS》1988,44(1):62-63
Summary The influence of steroid hormones on the induction of sister-chromatid exchange (SCE) in cultured CHO cells was studied. It was observed that estradiol-17 , estriol, estrone and ethynyl estradiol treatments enhanced SCE rates compared to the controls. Overall, these compounds produced a dose response effect. The importance of a detailed study on the long-term genetic effects of steroids on mammalian cells is emphasized.This work was supported by NIH-Minority Biomedical Research Support Program (MBRS), Grant No. RR 08124-11. The author is thankful for the technical assistance provided by NIH-MBRS student, Hoang Duong. 相似文献
16.
Effect of pulsing electromagnetic fields on DNA synthesis in mammalian cells in culture 总被引:3,自引:0,他引:3
DNA synthesis in Chinese hamster V79 cells was significantly enhanced when they were exposed to weak, pulsing electromagnetic fields generated by specific combinations of the pulse width (25 microseconds), frequency (10, 100 Hz) and magnetic intensity (2 X 10(-5), 8 X 10(-5) T). Conversely the DNA synthesis of cells in the fields at 4 X 10(-4) T was repressed to 80% of that in controls not exposed to the fields. 相似文献
17.
H. W. Renner 《Cellular and molecular life sciences : CMLS》1982,38(5):600-600
Summary Methylxanthines consumed daily by most humans were investigated for induction of sister chromatid exchanges (SCE). Effects
observed in this highly sensitive in vivo system decreased in the order theophylline/theobromine>caffeine >paraxathine>monomethylxanthines. 相似文献
18.
Although requirements for transformation in Branhamella catarrhalis are quite complex, DNA synthesis does not appear to be one of these needs, as indicated by the inability of nalidixic acid to interefere with transformation. Exogenous sources of energy, such as cAMP and cGMP also failed to enhance frequency, suggesting cells may actively engage in energy production to achieve uptake of DNA, or lack a transport mechanism for these compounds. 相似文献
19.
G Méténier 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1979,289(5):497-500
Results of autoradiographic studies of 3H-thymidine incorporation support a model of macronuclear DNA content regulation involving an action upon the extremes of DNA content: elimination of S phase in cells with large DNA content, additional S phase in cells with DNA content. The frequency of each of these phenomena is about 20%: the inequality of frequencies obtained with the two types of sister cells (proters and opisthes) is relatable to the asymetry of cytodieresis observed in this ciliate. 相似文献
20.
K. Takahashi I. Kaneko M. Date E. Fukada 《Cellular and molecular life sciences : CMLS》1986,42(2):185-186
Summary DNA synthesis in Chinese hamster V79 cells was significantly enhanced when they were exposed to weak, pulsing electromagnetic fields generated by specific combinations of the pulse width (25s), frequency (10, 100 Hz) and magnetic intensity (2×10–5 8×10–5T). Conversely the DNA synthesis of cells in the fields at 4×10–4 T was repressed to 80% of that in controls not exposed to the fields.This work was supported by Kaken Pharmaceutical Co., Ltd, Tokyo, Japan, and in part by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan and from the Foundation for Life Science Promotion Tokyo, Japan to I.K. 相似文献