百合基因转化受体系统的建立

以百合叶片为外植体不经愈伤组织直接诱导并且发育成带球径的大苗进行了研究,结果表明：叶片分芽培养基为ＭＳ＋ＢＡ０．５ｍｇ／Ｌ＋ＺＴ０．。１ｍｇ／Ｌ＋ＮＡＡ１．０ｍｇ／Ｌ生根培养基为１／２ＭＳ＋ＮＡＡ０．。２－１．０ｍｇ／Ｌ,成球并且生长培养基为ＭＳ＋ＩＡＡ１．０ｍｇ／Ｌ,移栽成活率为１００％《     

播娘蒿子叶及下胚轴诱导再生植株的研究

用揪娘嵩种子,在附加６－ＢＡ０．５～１．０ｍｇ／Ｌ＋ＮＡＡ０．１ｍｇ／Ｌ的ＭＳ培养基上获得无菌苗,幼苗的下胚轴在含６－ＢＡ０．１ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／Ｌ的ＭＳ培养基上的出愈情况最佳,将来自下胚轴的愈伤组织培养了含有６－ＢＡ２．０ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ的ＭＳ培养基上分化出的丛生芽状态最好,最佳生根培养基为１／２ＭＳ＋ＩＢＡ０．５ｍｇ／Ｌ＋ＮＡＡ０．０５ｍｇ／Ｌ。     

百合基因转化受体系统的建立

以百合叶片为外植体不经愈伤组织直接诱导并且发育成带球径的大苗进行了研究，结果表明：叶片分芽培养基为ＭＳ＋ＢＡ０．５ｍｇ／Ｌ＋ＺＴ０．１ｍｇ／Ｌ＋ＮＡＡ１．０ｍｇ／Ｌ，生根培养基为１／２ＭＳ＋ＮＡＡ０．２～１．０ｍｇ／Ｌ，成球并且生长培养基为ＭＳ＋ＩＡＡ１．０ｍｇ／Ｌ，移栽成活率为１００％．从而建立了百合基因转化的受体系统     

播娘蒿子叶及下胚轴诱导再生植珠的研究

用播娘蒿种子,在附加６－ＢＡ０．５～１．０ｍｇ／Ｌ＋ＮＡＡ０．１ｍｇ／Ｌ的ＭＳ培养基上获得无菌苗,幼苗的下胚轴在含６－ＢＡ０．１ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／Ｌ的ＭＳ培养基上出愈情况最佳,将来自下胚轴的愈伤组织培养于含有６－ＢＡ２．０ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ的ＭＳ培养基上分化出的丛生芽状态最好,最佳生根培养基为１／２ＭＳ＋ＩＢＡ０．５ｍｇ／Ｌ＋ＮＡＡ０．０５ｍｇ／Ｌ．     

风信子外植体植株再生系统的研究

风信子叶片及鳞片直接诱导下定芽的培养基为ＭＳ附加６－ＢＡ２．５ｍｇ／Ｌ、ＮＡＡ２．０ｍｇ／Ｌ、ＩＡＡ０．５ｍｇ／Ｌ和ＭＳ附加６－ＢＡ２．５ｍｇ／Ｌ、ＮＡＡ２．５ｍｇ／Ｌ。不定芽发育成小鳞茎的培养基为ＭＳ附加ＧＡ０．２ｍｇ／Ｌ、ＩＡＡ０．５ｍｇ／Ｌ。鳞茎移栽成活率为９０％,从而建立了风信子植株再生系统。     

郁金香叶片直接诱导试管人工种球的快速繁殖的研究

对郁金香的叶片直接诱导试管人工种球及发育成大球的快速繁殖途径进行了研究，结果表明：叶段在ＭＳ＋ＢＡ２．０ｍｇ／Ｌ＋ＮＡＡ２．０￣５．０ｍｇ／Ｌ中不经愈伤组织直接分化多个小鳞茎，Φ（Φ：鳞茎的直径）２ｍｍ的小鳞茎在ＭＳ＋ＧＡ１．０￣２．０ｍｇ／Ｌ中发育成带１￣２片叶的Φ５￣１０ｍｍ的大鳞茎，大鳞茎的移栽成活率为９８％￣１００％。从而建立了郁金香试管人工种球快速繁殖的新途径。     

雪兔子叶的组织培养及植株再生

以雪兔子无菌苗的幼叶为外植体,接种到ＭＳ补加ＮＡＡ（１．０－２．０）ｍｇ／Ｌ、６－ＢＡ（０．１－１．０）ｍｇ／Ｌ的培养基上诱导愈伤组织,其中以ＭＳ＋ＮＡＡ２．０ｍｇ／Ｌ＋６－ＢＡ０．５ｍｇ／Ｌ的诱导效果最好,经６周培养即可形成大量的黄绿色愈伤组织,诱导率可达１００％,经２－３次继代培养后,将生长发良好的愈伤组织转接到ＭＳ附加６－ＢＡ（０．５－２．０）ｍｇ／Ｌ、ＩＡＡ（０．０５－０．１）ｍｇ／Ｌ、Ｎ     

百合子房的组织培养

以百合子房为材料进行组培快繁，得到子房诱导成愈伤组织的最适培养基为ＭＳ＋ＢＡ１ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／Ｌ：适合诱导分化的培养基为ＭＳ＋ＢＡ０．５ｍｇ／Ｌ＋ＩＡＡ０．５ｍｇ／Ｌ；适合生根的培养基为ＭＳ＋ＢＡ０．２ｍｇ／Ｌ＋ＩＡＡ１ｍｇ／Ｌ．     

巴西铁的茎组织培养

以巴西铁（Ｄｒａｃａｅｎａ　ｓａｎｄｅｒｉｅｎａ）茎段为接种材料，ＭＳ为基本培养基，吲哚乙酸（ＩＡＡ）、茶乙酸（ＮＡＡ），２，４－二氯苯氧乙酸（２．４－Ｄ）、６－苄氨基嘌呤（６－ＢＡ）、６一糠基氨基嘌呤（６－ＦＡ）、吲哚丁酸（ＩＢＡ）、生根粉（ＡＢＴ１）、玉米素（ＺＴ）为附加成分，进行诱导、分化、生根试验。诱导结果表明：ＩＡＡ不起作用，２，４－Ｄ有较好的促进作用，０．５ｍｇ／Ｌ（２，４－Ｄ）＋２ｍｇ／Ｌ（６－ＢＡ）组合，诱导率高达９０％。分化结果表明：ＮＡＡ、６－ＢＡ效果较好，６－ＦＡ作用不明显，ｌｍｇ／ＬＮＡＡ＋４ｍｓ／Ｌ（６－ＢＡ）＋２ｍｓ／ＬＺＴ组合，分化率最高．达８１．１％。生根结果表明：ＭＳ＋０．５ｍｇ／ＬＮＡＡ＋０．５ｍｇ／Ｌ（６－ＦＡ），ＭＳ＋０．４ｍｇ／ＬＮＡＡ＋２ｍｇ／ＬＩＢＡ，ＭＳ＋０．２ｍｇ／ＬＮＡＡ＋０．１ｍｇ／Ｌ（６－ＦＡ）＋ＡＢＴ１三种培养基均发根，生根率７０％以上，根粗，但有点脆，若培养基中加入活性碳产生的根则细而韧。     

马铃薯遗传转化系统的优化及禽流感病毒(H5N1)基因的导入

通过对以马铃薯（Ｓｏｌａｎｕｍ ｔｕｂｅｒｏｕｓｍ Ｌ．）块茎为受体的农杆力菌转化系统进行优化,筛选出使三个品种（鲁引１号、克新３号、优金）的受体均可得高频分化的培养基：ＭＳ＋２．０ｍｇ／Ｌ ＢＡ＋３。０ｍｇ／Ｌ ＺＴ＋０．５ｍｇ／Ｌ ＮＡＡ＋０．５ｍｇ／Ｌ ＧＡ。对几种农杆菌转化条件的比较研究发现,菌体用Ｍｓ＋０．５ｍｇ／Ｌ ＧＡ流体重悬并稀释４倍屿受体于２８℃,１００ｒ／ｍｉｎ振荡侵染２０～３     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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Brief Introduction to “Journal of Jilin University( Science Edition) ”

<正>"Journal of Jilin University(Science Edition)"is a comprehensive academic journal in the fields of science sponsored by Jilin University and administrated by the Ministry of Education of the Peoples Republic of China.The journal started publication in 1955.The original name at starting publication was"Journal of Natural Science of Northeast People University",which was changed into"Acta Scientiarum Naturalium Universitatis Jilinensis"in 1958 owing to the name change of the university.The present journal name has