Identity of differentiation inducing factor and tumour necrosis factor

Human myelogenous leukaemic cells can be induced to differentiate into the monocyte/macrophage pathway by protein inducers called differentiation inducing factors (DIF) in conditioned media of mitogen-stimulated human peripheral blood leukocytes. However, human DIF has not yet been well characterized. DIF is known to be a T-cell lymphokine, as it can be obtained from the T-cell line HUT-102 and can be partially purified from medium conditioned by phytohaemagglutinin (PHA)-stimulated lymphocytes. We found that monocytes also produce factor(s) that induce differentiation of human myelogenous leukaemia cell lines to cells with macrophage-like characteristics. This factor(s) has activity different from that of colony-stimulating factor(s) or interferons. We have now purified a DIF to homogeneity from medium conditioned by PHA-stimulated leukocytes using a human myeloblastic leukemia cell line, ML-1, as target cells. The purified DIF has a relative molecular mass (Mr) of approximately 17,000, with an NH2-terminal sequence the same as that of human tumour necrosis factor (TNF). Recombinant human TNF (rHuTNF) induces differentiation of ML-1 cells and an anti-pDIF monoclonal antibody can neutralize both differentiation inducing activity and cytotoxic activity of DIF and rHuTNF. The findings indicate that one of the DIF(s) produced by leukocytes is probably TNF.     

因子分析法中因子得分的岭估计

从传统的因子分析模型出发，对其中的因子得分的回归估计运用了岭估计进行改进，从而有效增强了因子得分估计的稳定性．     

Identity of tumour necrosis factor and the macrophage-secreted factor cachectin

In mammals, several well-defined metabolic changes occur during infection, many of which are attributable to products of the reticuloendothelial system. Among these changes, a hypertriglyceridaemic state is frequently evident, resulting from defective triglyceride clearance, caused by systemic suppression of the enzyme lipoprotein lipase (LPL). We have found previously that macrophages secrete the hormone cachectin, which specifically suppresses LPL activity in cultured adipocytes (3T3-L1 cells). When originally purified from RAW 264.7 (mouse macrophage) cells, cachectin was shown to have a pI of 4.7, a subunit size of relative molecular mass (Mr) 17,000 and to form non-covalent multimers. A receptor for cachectin was identified on non-tumorigenic cultured cells and on normal mouse liver membranes. A new high-yield purification technique has enabled us to determine further details of the structure of mouse cachectin. We now report that a high degree of homology exists between the N-terminal sequence of mouse cachectin and the N-terminal sequence recently determined for human tumour necrosis factor (TNF). Purified cachectin also possesses potent TNF activity in vitro. These findings suggest that the 'cachectin' and 'TNF' activities of murine macrophage conditioned medium are attributable to a single protein, which modulates the metabolic activities of normal as well as neoplastic cells through interaction with specific high-affinity receptors.     

Variation properties of ionospheric eclipse factor and ionospheric influence factor

The concepts and calculation methods of ionospheric eclipse factor (IEF) and ionospheric influence factor (IFF) are further illustrated. The temporal an d spacial variation properties of IEF and IFF are studied, which shows that the properties are influenced by the geographic position and season. The possibility of improving the precision of using GPS data to determine ionospheric delay bas ed on the above variation properties is also analysed.     

一种基于比例因子的慢启动策略

TCP慢启动机制中拥塞窗口大小指数增长,会导致在慢启动前期发送速率偏低的现象。针对此问题,本文提出一种基于比例因子的慢启动策略,该策略实现了慢启动到拥塞避免的快速过渡,并保证了数据传输的总体效率。实验表明,新策略能明显提高网络吞吐量,利于网络资源利用。     

FRP板修复开裂钢板的应力强度因子影响因素分析

为系统地了解纤维增强复合材料(FRP)板修复损伤钢构件疲劳裂纹的影响因素,以FRP板修复含中心裂纹的钢板作为研究对象,以裂纹尖端的应力强度因子K作为分析指标,采用有限元方法对FRP板修复开裂钢板的K进行参数分析。结果表明:FRP板截面刚度、宽度和长度的提高能够使K降低,但当FRP板增加到某一长度后,进一步增加长度将基本不影响K;增加胶层剪切模量、降低胶层厚度可以降低K,局部剥离的出现导致K变大;FRP单面修复的K明显大于双面修复,增加FRP板厚度对降低单面修复的K作用不大。     

神经生长因子

概述了神经生长因子的分子结构及作用机制，综述了神经生长因子的生理功能，神经生长因子不仅对神经细胞的存活，生长发育，分化，再生和功能维持起调控作用。同时也能诱导细胞凋亡。     

Vaccinia virus encodes a polypeptide homologous to epidermal growth factor and transforming growth factor

Epidermal growth factor (EGF) and transforming growth factor type I (TGF) are polypeptides of 53 and 50 amino acid residues, respectively. Both bind to EGF receptor, a 1,200-residue transmembranous glycoprotein, leading to phosphorylation of the receptor, enhancement of its tyrosine-specific kinase activity and ultimately to stimulation of cell growth. We report here that a 140-residue polypeptide encoded by one of the early genes of vaccinia virus (VV) is related closely to EGF and TGF. The presence of putative signal and transmembranous sequences further suggests that the viral protein might be an integral membrane protein, but that, as in the case of EGF itself, the membrane-associated form may be the precursor of a soluble growth factor. Production of EGF-like growth factors by virally infected cells could account for the proliferative diseases associated with members of the poxvirus family such as Shope fibroma virus, Yaba tumour virus, and molluscum contagiosum virus (MCV).