Long term stability of lysine vasopressin and of specifically tritiated lysine vasopressin in weakly acidic aqueous solutions

Summary Lysine vasopressin tritiated at meta position of tyrosine (1.9 Ci/mmole) decomposes during a long storage period, mainly as a result of radiolysis. The loss of specific activity by isotopic exchange with water is slow. The nonlabelled peptide undergoes only very minute decomposition (polymerization). Specific radioactivity declines with a half life of 47.4 months.Acknowledgments. This research was supported by Swiss National Science Foundation, grant No.3.040.76 (V.P.). Some of the measurements (years 1969–70) were carried out at Roche Institute of Molecular Biology, Nutley, N.J., USA.     

Effect of human serum oxytocinase on the antidiuretic action of lysine vasopressin and oxytocin in the rat

Zusammenfassung Nach i.v. Dauerinfusion von Lysin-Vasopressin oder Oxytocin wird bei der experimentellen Analyse der Wirkung menschlicher Oxytocinase eine Erniedrigung des antidiuretischen Effekts gefunden; diese rührt vom Absinken der Peptidkonzentration sowohl im Blutplasma als auch am Wirkort her.     

Purification and stability of specifically tritium labelled lysine vasopressin (Tyrosine2-3-T-lysine8-vasopressin)

Zusammenfassung Tyrosin²-3-T-Lysin⁸-Vasopressin, das vor 2 1/2 Jahren synthetisiert worden war, wurde auf CM-Sephadex-C-25-Säulen gereinigt. Die spezifische Radioaktivität des spezifisch markierten Hormons war nur auf 80% gefallen, was einen verhältnismässig kleinen Grad von Isotopaustausch für das Nonapeptid selber anzeigt.     

Pyrogallol and vasopressin tachyphylaxis

Résumé Le pyrogallol empêche le développement d'une tachyphylaxie à la vasopressine. Il restitue la réaction pressorique après l'apparition d'une tachyphylaxie. Cet effet ne semble pas être lié à l'action inhibitrice de cette substance sur l'enzyme transférase o-méthyl.     

A radioimmunoassay of vasopressin. A note on pituitary vasopressin content in brattleboro rats

Zusammenfassung Die Empfindlichkeit und Zuverlässigkeit eines neu entwickelten Radioimmunotests für Vasopressin wurde durch Messungen des Vasopressinspiegels im Hypophysenhinterlappen von homozygoten und heterozygoten Ratten für angeborenen Diabetes insipidus geprüft und eine gute Korrelation zwischen dem biologisch und radioimmunologisch gemessenen Vasopressingehalt der Hypophyse nachgewiesen.     

Enzyme immunoassay for arginine vasopressin

Summary A highly sensitive enzyme immunoassay for arginine vasopressin (AVP) was described. The enzyme used was -D-galactosidase fromEscherichia coli, and it was coupled to AVP by using the N-hydroxysuccinimide ester of N-(4-carboxycyclohexylmethyl)-maleimide. The complex was then utilized for the enzyme immunoassay, which could detect 0.5 fmoles (or 0.5 pg) of AVP in the assay tube.     

Role of lysine side chains in metallothionein

Summary pK_a-Values of lysine residues of mammalian metallothionein were determined by chemical titration measurements of -CH₂ lysine resonances in the¹H-NMR spectra. They are about 0.5 pH-unit higher than the average pK_a-value of a metal-free derivative, suggesting interaction of the positively charged residues with the two three-fold negatively charged metal-thiolate clusters of the metal-containing form. Deprotonation of the lysines leads to circular dichroism changes attributable to an electrostatically induced structural transition of the protein.To whom correspondence concerning this paper should be addressed.     

On the inactivation of thrombin

Zusammenfassung Es wird eine Analogie zwischen der bekannten Thrombininaktivierung in Serum und dem Inaktivierungsprozeß von Thrombin durch gebundenes Heparin gezeigt. Es ist möglich, daß die Thrombininaktivierungsfähigkeit des Serums dem gebundenen Heparin zu verdanken ist.     

Effect of phospholipids on purified lipoamidase

Summary Phospholipids were added to purified lipoamidase from porcine brain microsomal membranes, and changes in lipoamidase activity were examined. Approximately twofold activation of lipoamidase activity occurred upon the addition of phosphatidylethanolamine. On the other hand, phosphosphatidylserine, cardiolipin, and phosphatidic acid reduced the enzyme activity by approximately 80%. This pattern of the activation of lipoamidase by phosphatidylethanolamine and its inhibition by phosphatidylserine is similar to the pattern for adenylate cyclase, and contrasts with the pattern for ATPase.     

Effect of phospholipids on purified lipoamidase

Phospholipids were added to purified lipoamidase from porcine brain microsomal membranes, and changes in lipoamidase activity were examined. Approximately twofold activation of lipoamidase activity occurred upon the addition of phosphatidylethanolamine. On the other hand, phosphatidylserine, cardiolipin, and phosphatidic acid reduced the enzyme activity by approximately 80%. This pattern of the activation of lipoamidase by phosphatidylethanolamine and its inhibition by phosphatidylserine is similar to the pattern for adenylate cyclase, and contrasts with the pattern for ATPase.     

Peptides related to vasopressin in invertebrates

Summary Compounds chemically related to the vertebrate neurohypophysial peptides, vasopressin and neurophysin, have been detected in recent years in the brain and in ganglia of invertebrates. Most data acquired so far have been obtained in insects and in molluscs. Evidence suggesting that these compounds might exert neurohormonal and neurotransmitter functions in these species is reviewed.Work by the authors is supported in part by Swiss NSF grant 3.560.083.     

Antibody against purified viper neurotoxin

Résumé L'anticorps contre la neurotoxine du venin deVipera palestinae est obtenu chez des lapins par immunisation de la neurotoxine purifiée liée à la cellulose carbométhylique.

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This work is part of a thesis submitted byN. Shaham to the

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This work is part of a thesis submitted byN. Shaham to the

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This work is part of a thesis submitted byN. Shaham to the

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This work is part of a thesis submitted byN. Shaham to the

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This work is part of a thesis submitted byN. Shaham to the