The effects of some tetracycline derivates on Ehrlich ascites carcinoma and HeLa cells in vitro

Zusammenfassung In-vitro-Nachweis der Hemmung der Proteo- und Nukleosynthese in den EAC-Zellen, sowie des Wachstums der HeLa-Zellen mit folgenden Derivaten des Tetrazyklins: Anhydrotetrazyklin, 4, 12-Anhydrodedimethylaminotetrazyklin und 12-Deoxydedimethylaminotetrazyklin.     

Serum ceruloplasmin in mice with Ehrlich ascites carcinoma

Résumé On montre que la présence du carcinome d'Ehrlich provoque une diminution statistiquement significative des taux de la céruloplasmine sérique des souris aux dixième et quatorzième jours après l'inoculation.

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One of us (R.R.A.) is working with a research grant from Conselho Nacional de Pesquisas (National Research Council of Brasil).     

Heterophile antigens in Ehrlich ascites tumor cells

Riassunto Gli autori hanno osservato che i sieri di animali immunizzati con cellule del tumore cancro ascite di Ehrlich sono emolitici per gli eritrociti di topo, ratto e montone e presumono che le cellule del tumore di Ehrlich possano contenere frazioni antigene di tipo eterogenetico.     

Incorporation of phospholipids in Ehrlich ascites tumor cells

Ehrlich ascites tumor cells (EATC) were incubated with unilamellar vesicles (UV) or multilamellar vesicles (MV). UV and MV were incorporated differently into EATC. The increase in 32P-phospholipid in EATC in the presence of UV was 12% in 300 min. Absorption of phospholipid from MV could account for only 3%. About 50% of the UV incorporation of 32P was by endocytosis and/or fusion.     

Incorporation of phospholipids in Ehrlich ascites tumor cells

Summary Ehrlich ascites tumor cells (EATC) were incubated with unilamellar vesicles (UV) or multilamellar vesicles (MV). UV and MV were incorporated differently into EATC. The increase in³²P-phospholipid in EATC in the presence of UV was 12% in 300 min. Absorption of phospholipid from MV could account for only 3%. About 50% of the UV incorporation of³²P was by endocytosis and/or fusion.This work was supported by a grant from the Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. J. L. Corchs is an investigator from the Consejo Nacional de Investigaciones Científicas y Técnicas.     

Interaction between mycoplasmas and Ehrlich ascites tumor cells

Zusammenfassung Es wurde gezeigt, dass die Adsorption von Mycoplasmen an Ehrlich Ascites-Tumorzellen an das Vorhandensein von neuraminidase-empfindlichen Zellrezeptoren gebunden ist.     

Effect of tannic acid on the Ehrlich ascites tumor cells

Summary Tannic acid was used to fix and stain Ehrlich ascites tumor cells in suspension. An increase in surface microvilli and cytoplasmic blebs in the tumor cells was observed. The mechanism of tannic acid induced surface morphological changes in tumor cells and the formation of a precipitate of protein-tannic acid-heavy metal complex are discussed.Acknowledgment. The author is grateful to Professor F. P. Lisowski for his advice and helpful criticism and to Mr Y. S. Tong for his expert technical assistance. This project was supported by a grant from the Hong Kong University Research Grants Committee.     

Glycerin prevents the 'heat-death' of Ehrlich ascites tumor cells

When heated to 45 degrees C for 20 min Ehrlich ascites tumor and NK-lymphoma cells are irreversibly damaged, so that they lose transplantability. The presence of 7-8% glycerol during heat treatment protects EAT cells completely and NK-lymphoma cells partly against this injury.     

Changes induced by Ehrlich ascites carcinoma in hepatic fumarase and aconitase activities

Summary Ehrlich ascites carcinoma induced significant changes in liver fumarase (activation) and aconitase (inhibition). A significant increase in hepatic fumarase specific activity was also observed in mice inoculated with Ehrlich ascites fluid after centrifugation of cells (EAF). Hepatic aconitase was not significantly influenced by EAF.R.R.A. is working with a research grant from the Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq), Brazil.     

Effect of tannic acid on the Ehrlich ascites tumor cells.

Tannic acid was used to fix and stain Ehrlich ascites tumor cells in suspension. An increase in surface microvilli and cytoplasmic blebs in the tumor cells was observed. The mechanism of tannic acid induced surface morphological changes in tumor cells and the formation of a precipitate of protein-tannic acid-heavy metal complex are discussed.     

Relationship between A-type and C-type particles in Ehrlich ascites tumor cells

Summary Intracisternal and intracytoplasmic A-type particles were discovered in Ehrlich ascites tumor cells. In addition, mature and immature C-type particles were also seen in the intercellular space. It is believed that A particles may represent a precursor or a formative stage of the C particles.Acknowledgment. The author is grateful to Professor F. P. Lisowski for his advice and helpful criticisms and to Mr Y. S. Tong for his expert technical assistance. This project was supported by a grant from the Hong Kong University Research Grants Committee.