高效液相色谱法测定纳豆及纳豆胶囊中的 大豆异黄酮含量

建立了纳豆及纳豆胶囊中大豆异黄酮的高效液相色谱分析方法,该方法重复性及样品稳定性良好.实验对原料黄豆、纳豆和纳豆胶囊样品采用石油醚索氏脱脂后,对固体样品进行乙醇回流提取,分析了4种大豆异黄酮组分,即大豆苷、染料木苷、大豆素和染料木素.结果表明,原料黄豆总异黄酮质量比为1 260 mg/kg,纳豆比原料黄豆总异黄酮含量明显高约30%,纳豆胶囊比原料黄豆总异黄酮含量高约11%.     

大豆胚芽异黄酮的提取及对食品抗氧化作用的研究

目的 为充分利用大豆资源,研究出更多的天然抗氧化剂.方法 采用大豆胚芽作为提取异黄酮的原料,设计正交试验方案,确定的最佳提取条件为:用80%的乙醇,按照乙醇体积与原料质量比10:1的比例在水浴100℃上回流2 h.称取40 g大豆胚芽,按最佳条件提取大豆异黄酮,产率0.694%.然后将提取的大豆异黄酮用于蛋糕制作,并按同一配方制作3种蛋糕,一种加入2 g山梨酸钾,一种加入大豆异黄酮2 g,另一种不加任何添加剂.成品后,将每一种蛋糕分两部分保存:一部分置于常温下保存,一部分置于冰柜中冷藏保存.1周后,测其过氧化值.结果 过氧化值比较:山梨酸钾＜大豆异黄酮＜无添加剂.结论 大豆异黄酮具有一定的抗氧化,且其作用效果较好.     

大豆中异黄酮类化合物提取工艺研究

采用多种工艺对大豆中的异黄酮类化合物进行提取。结果表明，以D－葡萄糖酸－б－内酯水溶液提取，乙醇沉淀除杂并结合DM130大孔吸附树脂注层析，能有效分离大豆异黄酮。提取物异黄酮含量361.6mg/g，收率0.413%。     

大豆中异黄酮提取条件及其在提取液中的存在形式

以大豆豆粕为原料，以总异黄酮为目标产物。采用正交试验设计探讨了用乙醇—水体系作溶剂提取大豆异黄酮的最佳条件，即体积分数为70％的乙醇，m乙醇：V水=0．1g／ml。50℃下提取3次，每次回流时间1．0h。采用酸水解法和等电点沉淀法研究了异黄酮在提取液中的存在形式。即水不溶性异黄酮占8.0%，水溶性异黄酮占92．0％．其中与糖结合的占61.3％，与蛋白质结合的占30．7％。为进一步分离异黄酮提供了试验基础。采用HPLC法测定异黄酮的量。     

酱油渣中大豆异黄酮动态逆流提取及分离纯化的研究

以酱油渣干粉为原料,对大豆异黄酮提取条件和纯化方法进行研究.采用实验室模拟动态逆流提取酱油渣中异黄酮,并通过正交设计对大豆类黄酮提取条件进行了优化,确定了大豆类黄酮的最佳提取条件为80%乙醇, 提取前的浸泡时间12h,料液比1:10,得到总异黄酮提取率达0.35%,粗提物的纯度为2.01%.选取3种极性不同的大孔树脂填装制备柱,并利用中低压色谱对大豆异黄酮样品进行纯化.结果表明,NKA9大孔树脂对总大豆异黄酮的纯化效果最好,在60%乙醇洗脱时,产品纯度可达到32%.该方法能实施在短时间内对大豆异黄酮样品进行大量的纯化,满足大豆异黄酮产品生产的需要.     

自主创新振兴中国大豆加工业

介绍了以我国大豆加工业的最大领域——油脂工业生成的、通常能用作饲料的高温豆粕为原料,提取高纯度大豆低聚肽（蛋白含量≥93%～95%、NSI值＝100%、溶解度＝100%,灰分≤15%）、高纯度大豆异黄酮（异黄酮总含量≥92%、G∶D≥8∶1）和大豆复合功能因子自主创新高技术;对我国大豆油脂加工业扭亏为盈,拉动豆农致富,对原料大豆“吃干榨净",实现资源节约型的大豆加工工业具有重要的指导意义和促进作用。     

超声波法在大豆异黄酮提取中的应用

本文通过实验分别得到了超声波法和加热回流法对大豆异黄酮的提取率,结果显示：提取30min后,用超声波法的提取率比加热回流法高了约12倍;然后通过做正交试验进一步确定超声波法对大豆异黄酮的最佳提取条件,即：提取时间为30min,乙醇浓度为70%,超声清洗机功率为450W,料液比为1∶15（g/mL）。     

酱油渣中大豆异黄酮的动态逆流提取及分离纯化研究

以酱油渣干粉为原料,对大豆异黄酮提取条件和纯化方法进行研究.采用实验室模拟动态逆流提取酱油渣中异黄酮,并通过正交设计对提取条件进行了优化,确定了最佳提取条件为体积分数为80%乙醇、提取前的浸泡时间12 h、料液比1∶10,总异黄酮提取率可达0.35%,粗提物的纯度为2.01%.选取3种极性不同的大孔树脂填装制备柱,并利用中低压色谱对大豆异黄酮样品进行纯化.结果表明,NKA9大孔树脂对总大豆异黄酮的纯化效果最好,在体积分数为60%乙醇洗脱时,产品纯度可达到32%.该方法能在短时间内对大豆异黄酮样品进行大量的纯化,满足大豆异黄酮产品生产的需要.     

大豆异黄酮提取过程中大豆蛋白变性的研究

使用不同含量的醇-水体系,不同温度和不同时间对从低温豆粕中提取大豆异黄酮和提取过程中大豆蛋白质的变性进行了研究。结合大豆蛋白的分离提出提取大豆异黄酮的最佳条件应为80%乙醇-水,在40℃下,提取1h。     

富含大豆异黄酮豆腐凝胶的研究

为了进一步改进豆腐加工工艺,减少大豆异黄酮流失,制备富含大豆异黄酮的豆腐,以豆腐得率和大豆异黄酮保持率为分析指标,考察食用胶添加量、LL-50A接种量、蛋白酶制剂添加量对大豆异黄酮保持率的影响.通过正交分析法得到最佳工艺条件为卡拉胶添加量1.25‰、LL-50A接种量0.03%、蛋白酶制剂添加量300,U/L.在此条件下,豆腐得率为213.43,g/100,g,大豆异黄酮保持率为2.52,mg/g.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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