Determination of the water content of nematode worms by interference microscopy

Zusammenfassung Das Interferenz-Mikroskop ist hier zum ersten Male bei Metazoen, nämlich lebenden Nematoden, zur Messung des Wassergehalts benutzt worden. Die Resultate werden beschrieben und die Möglichkeiten weiterer Anwendung diskutiert.     

Changes in dry mass of rat liver nuclei in chronic thioacetamide poisoning

Riassunto Dopo intossicazione con TAA, l'aumento di volume dei nuclei di fegato isolati è sostenuto sia da un accresciuto contenuto di acqua che di sostanze solide. Anche il rapporto fra sostanze solubili e insolubili è modificato.     

Postnatal variations of the dry mass of neuronal nuclei of the spinal cord in rat and guinea-pig

Riassunto Sono state studiate per mezzo del microscapio ad interferenza Baker-Smith le variazioni di massa secca e volume del nuclei dei neuroni del rigonfiamento lombare del midollo spinale nel ratto e nella cavia in rapporto all'età.

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This investigation was supported by a grant to this Department from the Consiglio Nazionale delle Ricerche (No. 04/76/4/3482).     

Neuronal and glial markers of the central nervous system

Summary This brief review evaluates the expression of cell-specific markers on differentiated neural cells and, where necessary, on their developing precursors. Within these limitations only the commonly used markers are discussed and those deemed unequivocal are only briefly appraised.     

Neuronal and glial markers of the central nervous system

This brief review evaluates the expression of cell-specific markers on differentiated neural cells and, where necessary, on their developing precursors. Within these limitations only the commonly used markers are discussed and those deemed unequivocal are only briefly appraised.     

Interrelations of glial cells and axons in the postembryonic development of the nervous system ofTubifex tubifex

Summary Transmission electron microscopy was used to study the role of gliosomes in the postembryonic development of the central nervous system in Tubifex (Oligochaetes).     

Fractlonation and characterization of cell nuclei from rat neocortex by protein-DNA ratios

Zusammenfassung Die Kerne des Rattenneocortex wurden mit einer nachBurdman modifizierten Methode auf einem diskontinuierlichen Saccharosegradienten in zwei Populationen aufgetrennt. Die Kerne werden durch das Protein/DNA-Verhältnis charakterisiert: kleine Kerne 1.5; grosse Kerne 3.9. Die morphologische Differenzierung im Phasenkontrastmikroskop erwies sich als ungenügend.

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This investigation was supported by grant No. 89 of the Fritz Hoffmann-La Roche Foundation.     

Serendipitous precise and unique staining of cell nuclei

Zusammenfassung Frischgefrorene Gewebesektionen verschiedener Gewebe wurden in Kalium-Eisen-Cyanid bei pH 5,2 inkubiert; dabei wurde eine präzise und einzigartige Färbung nur der Zellnuklei beobachtet. Die Ursache dieser selektiven Kernfärbung ist nicht verständlich, doch stellt sie höchstwahrscheinlich eine Färbung von Ferro-Verbindungen in den Nuklei dar.

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This investigation was supported in part by Public Health Service Research Grants Nos. CA 02624 and CA 02903 from the National Cancer Institute and in part by an institutional grant to the Detroit Institute of Cancer Research Division of the Michigan Cancer Foundation from the United Foundation of Greater Detroit, and Detroit General Hospital Research Corporation. Newaygo County Cancer Association. We wish to express our gratitude for stenographic assistance to Mrs.Georgette M. Bellmay.     

Carbohydrate interference of complement-dependent cell lysis

The antibody-mediated cytotoxicity of three autoreactive sera, an allogeneic hyperimmune serum and a xenogeneic hyperimmune serum was abrogated by the presence of either glucosamine, galactosamine, lactulose or lactose. This inhibition could be overcome in a dose-dependent fashion by increasing the amount of complement in the cytotoxicity assay, but not by increasing the amount of antibody. Furthermore, the inhibition was specific for these sugars in that isomers and N-acetylated derivatives were not inhibitory. The results suggest that these sugars directly blocked events of the complement cascade.     

Carbohydrate interference of complement-dependent cell lysis

Summary The antibody-mediated cytotoxicity of three autoreactive sera, an allogeneic hyperimmune serum and a xenogeneic hyperimmune serum was abrogated by the presence of either glucosamine, galactosamine, lactulose or lactose. This inhibition could be overcome in a dose-dependent fashion by increasing the amount of complement in the cytotoxicity assay, but not by increasing the amount of antibody. Furthermore, the inhibition was specific for these sugars in that isomers and N-acetylated derivatives were not inhibitory. The results suggest that these sugars directly blocked events of the complement cascade.