A simple enzymatic method for isolation of hepatocytes

Summary A new method is described for isolating hepatocytes by an enzymatic procedure: 0.05% bacterial collagenase in calcium-free Hanks' solution is injected into the liver tissue which is removed aseptically. The time required to prepare culture and risk of bacterial and fungal contamination is greatly reduced.     

Spectrophotometric method for estimating enzymatic synthesis of butyl oleate

Zusammenfassung Es wird eine einfache spektrophotometrische Methode für die Bestimmung der enzymatischen Synthese von Butyloleat beschrieben.     

Evidence for enzymatic reduction of 1-nitropyrene by rat liver fractions

Summary 1-Nitropyrene, a mutagenic compound found in diesel exhaust and photocopy toners, is reduced anaerobically by rat liver fractions to 1-aminopyrene. This reaction is stimulated in the microsomal fraction by NADPH and in the cytosol by FMN. Addition of both cofactors produced more reduction in S-9, microsomes, or cytosol than either cofactor alone.     

Disadvantages of acid precipitation of proteins during the assay of blood glucose by the enzymatic glucose oxidase-peroxidase system]

In the enzymatic procedure for blood sugar by means of glucose oxidase, acid protein precipitation of blood by perchloric acid or trichloracetic acid liberated oxidizing substances, which enhanced the coloration density in oxidizing the reduced chromogen of the reaction mixture, independently of the hydrogen peroxide generated from glucose, and would give false high values of glycemia, if additional precautions had not been taken. These substances, increasing considerably with times and temperature of blood conservation, would be of peroxide nature, and would accumulate in red blood cells during their exposure to air.     

Requirements for effective use of graphical methods in interactive forecasting

The construction of forecasts using interactive data analysis systems is greatly aided by the availability of graphical procedures. Data exploration, model identification and estimation, and interpretation of final forecasts are made considerably easier by the visual relay of information. This article discusses some recent developments in time series graphics designed to assist in the forecasting process. A discussion of requirerients for effective use of graphics in interactive forecasting is included as illustrated through an application of the Box-Jenkins methodology. Illustrations are included from the STATGRAPHICS system, a prototype implementation in APL.     

Necessity of adjuvants for inducing effective antibody response to zona pellucida antigens

Rhesus monkeys evoked a vigorous antibody response to a single heteroimmunization dose of zona pellucida antigens, when these were administered along with complete Freund's adjuvant. The antisera recognized all the three major porcine zona glycoprotein families and the animals were rendered amenorrheic after such immunization. Monkeys immunized with zona without adjuvant, however, failed to show any anti-zona antibody response and had normal menstrual cycles. Zona pellucida glycoproteins are thus not effective immunogens unless administered along with a powerful adjuvant.     

Necessity of adjuvants for inducing effective antibody response to zona pellucida antigens

Summary Rhesus monkeys evoked a vigorous antibody response to a single heteroimmunization dose of zona pellucida antigens, when these were administered along with complete Freud's adjuvant. The antisera recognized all the three major porcine zona glycoprotein families and the animals were rendered amenorrheic after such immunization. Monkeys immunized with zona without adjuvant, however, failed to show any anti-zona antibody response and had normal menstrual cycles. Zona pellucida glycoproteins are thus not effective immunogens unless administered along with a powerful adjuvant.