Combined action of nickel sulfate and x-irradiation on human lymphoblastoid leukemic cells]

In a first experiment, cells were cultured in media supplemented by nickel sulphate, irradiated in same media and cultured in same media after irradiation. In a second experiment, cells were cultured during 18 hrs. in media supplemented by nickel sulphate, and then cells were washed and cultured in normal media where they were irradiated. The nickel sulphate toxicity appears as a creasing function of the nickel sulphate concentration and the nickel sulphate action endurance. The nickel sulphate toxic effect is amplified by X-Rays. This amplification is a time function that depends on the X-Ray dose, nickel sulphate concentration and period of time from the outset of culture to the irradiation. The nickel sulphate toxic effect appears faster when nickel works after X-Rays.     

微生物技术在固体矿产资源开发中的应用

本文总结了国内外生物湿法冶金技术在低品位硫化铜矿、难处理金矿以及其他固体矿产资源中的研究现状,指出了存在的问题,并提出了未来的发展方向。     

Nickel-binding proteins

Nickel enzymes are a relatively new class of metalloenzymes. The seven known nickel enzymes are urease, hydrogenase, CO-dehydrogenase, methyl-coenzyme M reductase, Ni-superoxide dismutase, glyoxalase I and cis-trans isomerase. The requirement for nickel implies the presence of a nickel-processing system, since free transition metals are harmful to the cell. A nickel-processing system involves the recognition and transport of nickel into the cell and the handling of the nickel once it enters the cell until it is inserted into the nickel enzyme. Several mechanisms for nickel transport have been identified and will be reviewed here. Accessory proteins required for the biosynthesis of the nickel active site have been identified. Accessory proteins bind the nickel when it enters the cell and are proposed to assist with the insertion of nickel into the enzyme. The function of the characterized nickel-processing proteins is described, and models for nickel insertion into the nickel enzymes are presented.     

Nickel content of human lymphoblastoid cells cultured in a nickel sulfate-enriched medium]

Nickel is titrated by atomic absorption in nuclear and non-nuclear fractions of cells cultured in media supplemented by nickel sulphate. The living cell percentages are estimated in the same culture conditions. We try to connect the nickel presence in cellular fractions and the living cell percentages. These used cells seem to have mortality probability when nuclear fraction has 55 x 10(-6) of nickel and non-nuclear fraction has 33 x 10(-6) of nickel. We discuss the nickel stimulating effect for the start of DNA synthesis and the nickel penetration into cells.     

X-ray sensitivity of human cultivated lymphoblastic cells in media enriched in nickel sulfate]

Human leukaemic cells of the established line REH, cultured in media supplemented by nickel suplhate have been irradiated by X-Rays. The nickel sulphage and the X-rays have toxic effects that appear as independent or interdependent effects according to the X-ray doses, the nickel sulphate concentration and the nickel sulphate action endurances.     

Octopus chromatophores accumulate nickel

Summary Microprobe analysis of the pigment granules in the superimposed chromatophore layers ofOctopus vulgaris reveal rising amounts of calcium, nickel and sulfur as the size and electron-density of the granules increases. The colours, associated with these increases, shift from yellow/orange to red and finally black.This work was prepared while A.P. was on study leave in Germany supported by the Deutscher Akademischer Austauschdienst.     

Microorganisms seen by scanning and transmission electron microscopy in Legionnaires' disease from human lung.

In addition to several anomalous structures, other general forms of definitely rod-shaped microorganisms have been found by scanning and transmission electron microscopy in the lung tissue taken at autopsy from a patient who succumbed to confirmed Legionnaires' disease with extensive necrotizing lobar pneumonia. The microorganisms were greatly varied in size and shape. They were micrographed in the act of fission. These forms have been found to some extent throughout the tissue. No nickel was demonstrated, either in the lung tissue or in the microorganisms.     

Microorganisms seen by scanning and transmission electron microscopy in Legionnaires' disease from human lung

Summary In addition to several anomalous structures, other general forms of definitely rod-shaped microorganisms have been found by scanning and transmission electron microscopy in the lung tissue taken at autopsy from a patient who succumbed to confirmed Legionnaires' disease with extensive necrotizing lobar pneumonia. The microorganisms were greatly varied in size and shape. They were micrographed in the act of fission. These forms have been found to some extent throughout the tissue. No nickel was demonstrated, either in the lung tissue or in the microorganisms.This work has been supported in part by a Henry Ford Hospital institutional grant from the Ford Foundation and in part by the Veterans Administration Medical Center Research Funds.     

High levels of transition metals in dinoflagellate chromosomes

X-ray microanalysis of fixed, sectioned chromosomes of the dinoflagellates Glenodinium foliaceum, Prorocentrum micans and Amphidinium carterae has revealed high levels of iron, nickel, copper and zinc. We report high levels of these transition metals in association with chromosomes in intact eukaryote cells.     

Histamine et métaux cancérigènes

Summary In the case of mineral carcinogenic substances, the relation already established for organic carcinogenic substances with histamine is apparent. Silver, nickel, cobalt, chronium, and berryllium form well-characterized complexes with histamine.