人spindlin 1基因全长cDNA的克隆与定位

利用分子克隆技术和生物信息学手段,克隆并命名了人spindlin 1 基因. 此基因cDNA全长4375?bp,含有236～949 bp完整开放读框,预测编码27?ku 的膜内蛋白. 新基因在核酸序列上与鼠spindlin基因有96%同源,其编码氨基酸序列与鼠spindlin蛋白98%同源. 生物信息学分析表明该基因定位于9q22.1-22.3 区域, 基因组DNA全长为52.3?kb,由5个外显子和4个内含子组成,内含子和外显子之间的剪切完全符合GT-AG法则. 利用绿色荧光蛋白与spindlin 1基因的融合蛋白表达载体转染COS-7细胞表明spindlin 1表达的蛋白定位于胞核,且转染后的细胞中较多见胞核形态的改变,例如出现双核或巨型核.     

SH2D7基因及其编码蛋白的生物信息学分析

 为了对SH2D7 基因序列及其编码蛋白进行生物信息学分析,应用NCBI 数据库和ExPASy 等程序,对SH2D7 基因核苷酸序列进行了分析,并预测了其编码蛋白的理化性质及二级结构等。结果表明,SH2D7 基因定位于人类染色体15q25.1,包含了6 个外显子和5 个内含子。SH2D7 基因编码蛋白的功能主要涉及基因表达调控、信号传导等;分子量约为49.8 kD,等电点为5.99,是一种亲水蛋白,主要位于细胞核中,有35 个磷酸化位点及22 个抗原位点。     

苦荞CCT基因家族生物信息学及表达分析

CCT转录因子在调控植物花期、生长发育及抗非生物胁迫等方面发挥着重要的功能。本研究以拟南芥AtCCT基因家族为参考序列,利用本地BLAST并结合保守结构域等生物信息学工具,筛选出苦荞FtCCT基因家族成员,并对其理化性质、染色体分布、基因结构、系统进化及表达水平进行分析。结果显示:从苦荞中共鉴定出35个FtCCT基因,含1-8个内含子;编码蛋白有117-753个氨基酸残基,等电点为4.96-9.51,均为亲水性蛋白。染色体定位分析表明,这些基因在8条染色体上均有分布。苦荞FtCCT基因家族含有10个保守基序和5个保守结构域,且都含有CCT保守结构域。系统进化分析表明,苦荞的FtCCT基因家族与拟南芥一样可分为3个亚家族,其中CMF亚家族的成员最多。35个FtCCT基因在苦荞根、茎、叶和花中的表达水平具有差异性,在叶和花中具有高表达量的成员较多,只有少数的成员在根和茎中高表达。本研究为进一步解析CCT基因调控苦荞花期及生长发育奠定基础。     

4种竹子木质素合成酶PAL的基因克隆和序列分析

苯丙氨酸解氨酶（Phenylalanine Ammonia—Lyase,PAL;EC4．3。1．5）是木质素生物合成过程的关键酶和限速酶。应用RACE（Rapid—Amplification of cDNA Ends）方法,获得了白哺鸡竹（Phyllostachys dulcis）、高节竹（Phyllostachys prominens）、龙鳞竹（Phyllostachys pubescens heterocycla）、丽水苦竹（Fveioblastus maculosoides Wen）等4种竹子PAL基因的全长序列,并进行了生物信息学分析。结果表明,PAL基因的开放读码框长度为2136bp,共编码712个氨基酸,具有2个外显子和1个内含子。其中高节竹、龙鳞竹、丽水苦竹PAL基因内含子长度为121bp,白哺鸡竹朋L基因内含子长度为84bp。推测其氨基酸序列,并分析PAL蛋白单体的三维结构,结果显示PAL蛋白均含有大量的α螺旋和β折叠结构。基于邻接法的进化树对31个物种的PAL基因的氨基酸序列分析表明。竹类植物PAL基因的保守性较高,与禾本科植物玉米、甘蔗的亲缘关系较近,与双子叶植物辣椒等的亲缘关系较远。     

小立碗藓CAS基因的克隆及与拟南芥CAS基因的比较

钙信号在动植物的生长、发育过程中具有不可替代的作用.胞外钙受体(Extracellular Calcium-sensing receptor,CAS)是动植物质膜上一种跨膜离子通道蛋白,可以感受胞外Ca2 水平,充当第一信使将胞外的信号传递到细胞内.但迄今为止,仅2003年从植物拟南芥中克隆了胞外钙受体CAS基因,其起源进化我们知之甚少.本实验成功克隆了小立碗藓CAS基因,其cDNA全序列共1 574 bp,5′-非翻译区80个核苷酸,3′-非翻译区234个核苷酸,开放阅读框(ORF)1 257 bp,编码419个氨基酸,共有6个内含子.虽然藓类植物与被子植物进化距离较远,但小立碗藓CAS基因前五个内含子的插入位置以及相位与拟南芥完全相同,这说明植物中CAS基因在漫长的进化过程中非常保守,暗示被子植物胞外钙离子信号感受、传递机制起源于藓类植物.     

拟南芥翻译延伸因子EF1A的亚细胞定位研究

探讨了拟南芥的翻译延伸因子EF1A的亚细胞定位.以Col野生型拟南芥的cDNA为模板,通过高保真KOD扩增酶获得2 363bp的拟南芥转录延伸因子EF1A基因片段,将其与GFP融合后共同连入p1300表达载体,利用农杆菌侵染法将p35S∶GFP和p35S∶EF1A∶∶GFP转入野生型拟南芥中,观察转基因植物细胞中GFP的表达情况.结果显示:p35S∶GFP转基因植物中,细胞核中GFP荧光强度显著高于细胞质中荧光强度,但是在p35S∶EF1A∶∶GFP转基因植物中,细胞核中荧光强度与细胞质中荧光强度无显著差异,说明在进行蛋白翻译时细胞质中的EF1A含量显著高于细胞核中,这也为翻译延伸因子EF1A的亚细胞定位提供了实验依据.     

何首乌中一种Ⅲ型PKS基因的克隆及生物信息学分析

Ⅲ型聚酮合酶即查耳酮合酶超家族,能催化生成一系列结构各异、具有不同生理活性、含有查耳酮合酶基本骨架的植物次生代谢产物。根据不同植物Ⅲ型PKSs基因的保守序列,设计简并引物,采用RT - PCR和RACE技术,首次从传统中药材何首乌(Polygonum multiflorum Thunb.)中克隆到一种Ⅲ型PKS基因,其cDNA全长1228bp, 编码379个氨基酸,命名为FmPKS (GenBank登录号: GQ411431)。该基因含有三个内含子, 与迄今报道的绝大部分Ⅲ型PKS含有一个内含子不同,而与最近报道的虎杖中克隆的PcPKS2基因相同。通过生物信息学方法对其编码蛋白的序列进行同源性分析,构建了系统进化树,并对其等电点、疏水性及二级结构、跨膜区域等理化性质进行了初步预测,为进一步研究其功能奠定了基础。     

重组拟南芥细胞色素P450 707A3在大肠杆菌中的原核表达

拟南芥细胞色素P450 707a基因是编辑脱落酸8-羟化酶的基因,克隆其中的cyp707a3基因并构建表达载体p CWori(+)-cyp707a3,在E.coli Rosseta菌株中原核表达并得到了具有活性的酶蛋白.结果显示:大部分酶蛋白定位于大肠杆菌细胞膜.     

诸葛菜(Orychophragmus violaceus)Toc33基因编码区的克隆及序列分析

以诸葛菜（Orychophragmus violaceus）新鲜嫩叶为材料提取总DNA，并以其为模板，根据已报道的拟南芥（Arabidopsis thaliana）Toc33基因的编码序列，设计一对PCR引物，扩增诸葛菜叶 本外膜蛋白转运器构件蛋白基因Toc33，得到两条扩增带，将这两条带分别割胶回收，与T载体连接转化E.coli，筛选重组子并测序，结果表明克隆到的这两个片段分别长1475bp，1573bp，通过同源性比较发现，它们之间的同源性达到88％，这两个片段与拟南芥Toc33基因有较高的同源性，分别命名为OvToc33-1,OvToc33-2。参考拟介Toc33外显子和氨基酸序列，分别确定了所克隆的两信诸葛菜Toc33基因片段的7个外显子和6个内含子，得到了诸葛菜Toc33基因的全编码区序列，并推导了其氨基酸序列，这两个DNA序列与拟南芥Toc33基因编码区的同源性分别高达91.8%和92.4%，说明这一蛋白是高度保守的。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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