白酒丢糟的多菌种混菌固态发酵制备有机肥

以白酒丢糟为主要原料,4种芽孢杆菌、2种霉菌与酿酒酵母为混菌发酵菌株,进行多菌种混菌固态发酵,以发酵产物的腐殖质、全氮、全磷、全钾含量为指标,通过单因素实验和响应面法分析探讨酒糟固态发酵制备有机肥在初始含水量、酒糟量和初始pH的最佳工艺条件.经验证性实验可知预测值与实际值相符,得到最佳工艺条件:初始含水量为60.54%、酒糟量为52.06%、初始pH为7.03,接种量为3%~5%,发酵时间为6 d,发酵产物中腐殖质含量达到17.50%,种子发芽指数GI值达到93.2%,E4/E6值为2.19.     

微生物发酵转化黄芩苷生成黄芩素的研究

葡萄糖醛酸酶的菌株HQ10,能够以黄芩为底物,通过发酵将黄芩中的黄芩苷转化为黄芩素. 通过对发酵条件进行优化,黄芩苷转化率近于92%. 发酵产物经TLC、HPLC、质谱等方法检测确定为黄芩素.     

红芪药渣固态发酵生产蛋白饲料的探讨

蛋白饲料的严重短缺是全球性问题,新型蛋白饲料资源开发势在必行.开发以中药渣为原料的天然、环保、绿色饲料资源迫在眉睫.本文以大量学术文献为依据,优选甘肃省地道药材红芪药渣进行固态发酵生产蛋白饲料,研究证实,中药渣蛋白饲料保健功效好,能增强动物机体免疫力、促进生长发育和新陈代谢、改善肉质、提高禽类产蛋率和减少饲料消耗等功效.红芪药渣排放量较大,营养和药用价值较高,将其资源化利用,固态发酵制备蛋白饲料等,既节约又环保,更有经济效益,有利于本地畜牧、养殖业的发展.     

青霉T24-2降解甘蔗渣产糖试验的条件优化研究

青霉（Penicillium sp.T24-2）能利用甘蔗渣固态发酵产纤维素降解酶。介绍了在影响青霉T24-2降解甘蔗渣产糖实验的条件下,用方差分析对实验的数据进行分析和处理,确定最佳实验条件,从而达到优化实验条件的目的。     

生物农药固态发酵条件的研究

探讨了以麸皮为主要原料进行固态发酵生产生物农药的方法,研究了不同培养基成分及培养条件对苏云金杆菌毒力效价的影响．试验结果表明,最适发酵培养基为：麸皮1000g,玉米粉20g．豆粕150g,KH2PO4 2g;最佳发酵条件为：原料含水量为60％,发酵温度30℃、pH为7．5,培养时间48h．结果分析表明,苏云金杆菌的毒力效价达5400IU／mg。     

赤霉素的固态分批补料培养技术及其分离提取工艺研究

经菌种筛选来串珠镰孢AS．3,2960菌株;对影响赤霉素产率的固态分批补料发酵的7个因素进行了正交试验,从而筛选出最佳发酵条件为：在32℃下,按培养基初始pH值4．5,接种量20％的比例,在第3天添加13．2％的玉米淀粉,从第3通气,共培养6d;对固 发批补料发酵与传统的液态发酵产生赤霉素能力进行了比较,结果表明：其固态分批补料培养技术为液态发酵的3－4．7倍;对发酵产物进行了分离提取效果的比较实验,结果表明：以10％的乙醇,在pH2．5下提取效果最佳;进行了多级逆流浸提试验,赤霉素含量达到0．70g/kgDMB（发酵物）。     

以全燕麦为基质的干酪乳杆菌Zhang固态发酵研究

以燕麦全粉为唯一基质进行干酪乳杆菌(Lactobacillus casei)Zhang固态发酵,以期获得一种具有益生元功效的谷物发酵食品.为了提高干酪乳杆菌的活菌数,对燕麦固态培养基进行了优化,选择含水量、接种量、发酵时间和培养基初始p H进行单因素实验,通过正交实验进一步优化得到最佳发酵条件.结果表明:含水量55%、接种量9%、培养基初始p H 6、37,℃静置培养36,h为最佳条件,此时干酪乳杆菌活菌数为6.32×109,,g-1,滴定酸度为9.93,m L,乳酸含量为0.45,g/100,g.对燕麦发酵前后的营养成分进行分析可知,发酵后β–葡聚糖含量为1.61,g/100,g,和发酵前相比几乎没有降低,α–氨基氮的含量增加了0.06,g/100,g,相对分子质量小于6,000的多肽增加了10.62%.     

赤霉素的固态分批补料发酵与传统的液态发酵比较试验

用串珠镰孢ＡＳ,３,２９６０菌株,在相同条件下,进行赤霉素的固态分批补料发酵和液态发酵的比较试验。结果表明：固态分批补料发酵赤霉素产量为液态发酵的３－４．７倍。     

黑曲霉和热带假丝酵母混菌固态发酵麸皮工艺优化

为探索微生态发酵饲料固态发酵的最佳工艺参数,以黑曲霉和热带假丝酵母作为发酵菌种,以麸皮作为主要发酵基质,进行混菌发酵,以提高麸皮的蛋白含量和减少麸皮的粗纤维含量为衡量指标,优化工艺参数.最佳工艺参数为:麸皮含水量70%,黑曲霉与热带假丝酵母菌种配比为2∶1,发酵温度32℃、发酵时间72h.在此工艺参数下固态发酵麸皮发酵效果最好,蛋白质含量最高,粗纤维含量最低.     

纤维素降解木霉菌株的筛选及其生物学特性探究

选取分离自河北安国中药材种植基地不同药用植物根区土壤的7株木霉菌,通过羧甲基纤维素钠培养基初筛及玉米秸秆、甘草药渣固态发酵培养基复筛,联合筛选纤维素降解木霉菌株,采用形态学和分子生物学相结合方法进行菌株鉴定,并对其生物学特性进行探究.结果表明,经羧甲基纤维素钠培养基初筛,有4株菌表现出纤维素降解能力,分别为HQ1、BB1、DS3和DS1.经2种木质纤维素底物固态发酵培养基复筛发现,BB1以玉米秸秆为发酵基质时滤纸纤维素酶(filter paper cellulase,FPase)活性最高,HQ1以甘草药渣为基质时FPase活性最高.结合菌落形态、显微结构和DNA分子鉴定,HQ1被鉴定为长枝木霉(Trichoderma longibrachiatum),BB1为非洲哈茨木霉(Trichoderma afroharzianum).2株菌生物学特性存在趋同性,适宜生长产孢pH为5~6,适宜培养温度为28~33 ℃,且都表现出抵御干旱胁迫的能力.变差分解表明,不同培养条件对木霉菌生长速率及产孢有重要影响.本实验可为后续进一步优化HQ1和BB1降解不同木质纤维素底物固态发酵培养条件提供依据.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

正~~