内部审计--内部控制的控制

内部审计既是企业内部控制系统的一个部分，也是控制内部控制其他环节的主要力量。它在加强企业管理、协调企业控制工作中具有不可替代的地位。加强内部审计工作就是从更高的层次完善企业的内部控制制度，内部审计是企业内部控制的控制。     

Enhanced structural stability of adenovirus nanocapsule

Application of viral vector in gene therapy and vaccination is still limited by their structural stability, which significantly increased avoidable cost in storage and transportation. Herein a non-covalent conjugated low-pH degradable nanocapsule has been adopted to stabilize viral vectors. By utilizing a luciferase expressing adenovirus, AdCMVLuc, we succeeded in a raise of over 11 folds in AdCMVLuc’s structural stability after 12 days storage at 4 1℃.     

腺病毒同源重组子的鉴定

携带入ＩＦＮ－γｃＤＮＡ序列的Ｅ１区缺失的腺病毒载体ｐＡｄｌ２／ＲＳＶ－ＩＦＮ－ｂｐＡ与ｐＪＭ１７质粒，通过磷酸钙沉淀法共转染２９３细胞，经同源重组，共获得６个病毒噬斑。病毒噬斑感染２９３细胞，至出现完全细胞病变效应（ＣＰＥ）后，抽提细胞内的病毒ＤＮＡ，经ＸｈｏⅠ酶切后走凝胶电泳，出现重组腺病毒圾的３．５ｋｂ大小的ＤＮＡ条带，＾３２Ｐ标记的探针ｐＡｄｌ２／ＲＳＶ－ＩＦＮ－ｂｐＡ与ＸｈｏⅠ酶切过的病     

Covalently closed circles of adenovirus 5 DNA

The genome of adenoviruses is a double-stranded linear DNA molecule with inverted terminal repeats about 100 base pairs (bp) in length and a terminal protein covalently linked to the 5' nucleotide of each strand. Both of these features permit the formation of DNA circles, the inverted repeats allowing the circularization of single-stranded DNA and the terminal protein the joining of one or more molecules to yield double-stranded circles or concatemers. However, although the existence of covalently closed circles has been postulated, double-stranded viral DNA purified from virions or infected cells by conventional methods (that is, using proteases and phenol or chloroform) has always been obtained in a linear form. Here, we present evidence for the existence in adenovirus 5 (Ad5) infected cells of novel structures resulting from covalent head-to-tail joining of viral DNA molecules and show that these structures are due at least in part to the formation of covalently closed circles.     

基于内模控制的内部热耦合空分策略

内部热耦合空分塔（ITCASC）在空分流程中具有很大的节能前景,是内部热耦合技术应用的突破点。由于该过程具有很强的非线性和复杂的动态特性以及耦合性,导致控制方案的设计具有极大的困难,有效的控制方案是该技术广泛应用的基础。文中提出了一种降阶模型,并在此基础上建立了ITCASC内模控制方案。结果表明,该控制方案能很好地解决非线性和耦合性,是一种比PID更有效的ITCASC控制策略。     

主成分回归的主成分选择准则

结合回归自变量选择的Cp准则及平均残差平方和准则，讨论了利用主成分回归时如何选择主成分可在一定程度上避免人为性，提出了两个准则，以供实际应用时参考。     

企业内部环境与内部控制有效性研究

内部环境是企业内部控制有效执行的必要保证.健全的治理结构是内部控制有效执行的基本前提;企业文化通过意识观念的渗透和同化,影响着企业的内部控制;人力资源是内部控制科学有效与否的重要决定因素.一些企业因为缺乏良好的内控环境而造成巨大损失.所以必须梳理内部环境.提升企业内部控制执行效果.