2型糖尿病患者骨保护素水平变化及与氧化应激的关系

目的:探究2型糖尿病患者骨保护素(OPG)水平变化与氧化应激之间的关系.方法:以我院30例2型糖尿病患者为研究对象,将其作为研究组,另外选择30例健康人作为对照组.采用双能X线骨密度法测定患者骨保护素水平、采用ELISA法检测患者中血清MOD与GSH-PX的活性,并测定MDA含量.结果:检测后发现2型糖尿病患者的血清中SOD、GSH-PX活性值为(0.85±0.04)ug/mL,(126.95±1.12)ug/mL低于对照组的(12.84±0.16)ug/mL、(295.6±0.24)ug/mL(P0.05);MDA含量(24.95±2.21)ug/mL高于对照组(1.64±0.05)ug/mL(P0.05);OPG含量为(0.26±0.05)ug/m,高于对照组患者的(0.11±0.06)ug/mL.结论:OPG的变化水与患者体内的应激状态密切相关,临床治疗中可以通过这一环节改善患者的治疗效果.     

纤维蛋白原和D-Ⅱ聚体含量变化在心肌梗塞中的应用

目的 :探讨纤维蛋白原 (Fg)和D—Ⅱ聚体(D -dimer)浓度变化在心肌梗塞中的应用价值。方法 :纤维蛋白原 (Fg)定量采用CA530型血凝仪 ,D—Ⅱ聚体定量采用AU560型全自动生化仪 ,其结果与患者和对照组水平做对比分析。结果:AMI患者溶栓前Fg浓度为4.42±0.36(g/L)、D -dimer浓度2.48±0.92(ug/ml)及阳性百分率为68.6 %。溶栓期 ,Fg浓度1.32±0.18(g/L)、D -dimer浓度4.16±0.89(ug/ml)及阳性百分率为86.8% ;恢复期 ,Fg浓度2.73±0.24(g/L)、D -dimer浓度1.18±0.62(ug/ml)及阳性百分率43.3 %。各值与对照组Fg浓度2.95±0.48(g/L)、D -dimer浓度0.29±0.23(ug/ml)及阳性百分率5.0% ,有显著性差异 (P<0.01)。结论 :血浆Fg浓度和D -dimer浓度及阳性百分率水平升高是AMI发病的重要危险因素 ,与AMI的发生和发展密切相关。在溶栓治疗监测中纤维蛋白原浓度和D -Ⅱ聚体浓度及阳性百分率变化成为重要的临床生物学指标。     

基于DNA-AuNCs的荧光传感新方法灵敏检测胰蛋白酶活性

以DNA模板金纳米簇(DNA-AuNCs)作为荧光探针,发展了一种灵敏检测胰蛋白酶活性的荧光分析新方法.在胰蛋白酶存在条件下,牛血清白蛋白水解成多肽片段,释放出游离的半胱氨酸残基.半胱氨酸残基与金纳米簇通过Au-S键形成非荧光的配合物,导致金纳米簇的荧光强度显著降低.该方法对胰蛋白酶的线性检测范围为0.1～2.0mg/L,检出限为20μg/L(R_(SN)=3).此外,该方法被成功应用于人血清样品中胰蛋白酶含量的测定.     

重型病毒性肝炎患者血清甲胎蛋白检测的意义

目的探讨重症肝炎患者血清甲胎蛋白(AFP)变化与预后的关系.方法采用化学发光免疫分析技术,检测30例重型肝炎患者血清 AFP.结果患者血清 AFP 呈高水平者(＞400μg/L)预后良好;血清AFP呈低水平者(＜100μg/L)常病情恶化.结论观察血清AFP的动态变化对判断重型肝炎的预后有参考价值.     

火焰原子吸收法测定牙釉质中铜、锌、铁含量

本文用火焰原子吸收法测定牙釉质中铜、锌、铁含量。测定健康者26人,铜、锌、铁平均值分别为16.4±6.5ug/g,325.8±84.8ug/g,34.7±9.1ug/g;龋病患者54人,铜、锌、铁平均值分别为16.1±5.4ug/g,269.9±63.5ug/g,38.7±10.0ug/g。测定结果表明,龋病患者牙釉质中的铜略低于正常者,锌明显低于正常者,而铁略高于正常者。该方法的回收率铜为98.25～103.3％,锌为97.0～101.5％,铁为97.2～102.4％;相对标准偏差铜小于2.79％,锌小于2.60％,铁小于2.10％。     

双酚A对泥鳅卵黄蛋白原的诱导

研究了不同浓度[（0．1—500）μg／L）]、不同时间（7d,14d）BPA暴露对雄性泥鳅卵黄蛋白原的诱导效应。结果表明,雄性泥鳅血清Vtg含量随BPA暴露时间和暴露浓度的增加而显著增加。暴露14d后,诱导雄性泥鳅血清Vtg显著性增加的最低BPA浓度为1μg/L。     

化工区成年人体内β_2-m含量观察

本文对416例化工区居住5年以上者进行了血清β_2－m含量测定，其结果观察组为2．11±0．48μg/ml；对照组为1．96±0．51μg/ml（P＜0．01）。另外观察组与对照组之间，男性为2．14±0．51μg/ml；1．95±0．477μg/ml（P＜0．01），女性为2．0±0．46μg/ml，1．98±0．54μg/ml（＜0．05）。实验结果表明，化工区成年人机体内β_2－m含量有不同程度的改变。     

CCl_4接触者SCG水平探讨

COl4为侵肝性毒物，为了解CCl4作业人员SCG水平，本文采用RlA法对92例接触者进行了SCG测定，其结果表明接触者和正常对照组SCG水平分别为198±173．41μg/L和105±87．1μg/L，它们之间有极显著性差异（P＜0.001）。实验结果还表明，接触2－5年者异常率为24．4％；6－13年者异常率为54．9％。由此可见CCl4接触者sCG水平有明显改变，与接触时间长短有密切关系。     

血清AFP临界值判断对肝癌诊断的影响

为探讨血清AFP诊断肝癌的合理临界值,本研究收集肝癌患者35例、正常对照者35例。血清AFP检测采用电化学发光法和酶联免疫吸附法(ELISA)定量,结果用t检验和χ2检验进行统计学分析。2种方法检测血清AFP水平显著相关,相关系数为0.978,检测值无显著性差异,P>0.05。二者ROC曲线下面积分别为0.890、0.880,说明血清AFP水平对肝癌诊断价值较高。按照约登指数最大原则确定血清AFP电化学发光法诊断肝癌的临界值为3.25μg/L,其敏感度为74.3%,特异度为94.3%,准确度为84.3%;ELISA法诊断肝癌的临界值为1.118μg/L,其敏感度为62.9%,特异度为100%,准确度为81.4%。而以目前常规临界值20μg/L来分析,则电化学发光法检测AFP诊断肝癌的敏感度仅为45.7%,特异度100%,准确度72.9%;ELISA法敏感度48.6%,特异度100%,准确度74.3%。因此合理降低临界值可有效提高血清AFP水平对肝癌的诊断价值。     

过氧化物酶活性部位的模拟及其催化显色反应的研究

本文合成了锰(Ⅲ)与meso-四(4-磺基苯)卟啉的螯合物(Mn-TPPS_4),研究了它作为过氧化物模拟酶对过氧化氢、4-氨基安替比林与苯酚显色反应的催化活性。拟订的光度法可准确地测定0～6μg/ml H_2O_2含量,1.5μg、8.0μg和40μgH_2O_2的平均回收率依次为95％、96％和100％。将显色反应与葡萄糖氧化酶催化反应偶联,测定了人血清中葡萄糖的含量,取得了满意的结果。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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