Quantitative paper chromatography of lipids

Zusammenfassung Ein qualitatives Verfahren zur papierchromatographischen Trennung von Fettsäuren und Derivaten liess sich zur quantitativen Bestimmung ausbauen. Die Methode hat eine Fehlergrenze von etwa ± 5% und ist unabhängig von den funktionellen Gruppen der Moleküle. Sie ist für gesättigte Di- und Triglyceride nicht direkt anwendbar.

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This work has been supported by a research grant from the National Institutes of Health (P. H. S. 4226) and by the Hormel Foundation. Hormel Institute publication No. 143.     

Affinity chromatography of glucose dehydrogenase

Summary Porcine liver -d-glucose dehydrogenase, a multi-functional protein, has been purified to apparent homogeneity. The enzyme has been separated from the endoplasmic reticulum using Triton X-114 and further purified using NAD to release glucose dehydrogenase from a NADP-linked sepharose column. The purified enzyme is capable of producing both NADH and NADPH in vivo as indicated by kinetic studies.     

Affinity chromatography of monoamine oxidase

Summary A method is described for the purification of pig liver monoamine oxidase by affinity chromatography, using a colum with covalently bound pargyline.Acknowledgment. The authors wish to thank the University Research Council for financial support.     

Affinity chromatography of glucose dehydrogenase

Porcine liver beta-D-glucose dehydrogenase, a multi-functional protein, has been purified to apparent homogeneity. The enzyme has been separated from the endoplasmic reticulum using Triton X-114 and further purified using NAD to release glucose dehydrogenase from a NADP-linked sepharose column. The purified enzyme is capable of producing both NADH and NADPH in vivo as indicated by kinetic studies.     

Thin layer chromatography of catecholamines and their metabolites

Zusammenfassung Eine einfache, schnelle und empfindliche Methode zur dünnschichtchromatographischen Trennung von 0,2–1µg Menge von Noradrenalin, Adrenalin, Normetanephrin, Metanephrin, 3,4-Dihydroxy-mandelsäure und 3-Methoxy-4-hydroxymandelsäure wird beschrieben.     

Synthesis of specific cholinergic inhibitors for affinity chromatography

Summary Our first aim was to simplify the spacer-synthesis for affinity-chromatography of cholinergic proteins. Further-more we synthesized 2 new inhibitors which proved to be useful for purification of acetylcholine-receptor protein.Dedicated to Prof. Peter Gaudenz Waser on the occasion of his 60th anniversary.     

Separation of specific fractions of synaptosomes by affinity chromatography

Summary The authors describe a new technique for isolation of specific fractions of synaptosomes, on the basis of their surface glycoproteins, by affinity chromatography using lectin-Sepharose columns.     

Strip-transfer method applicable in two-dimensional paper chromatography

Zusammenfassung Methode zur Befestigung eindimensionaler Papierchromatogramme an anderen Chromatogrammblättern. Dies ermöglicht eine zweite Trennung senkrecht zur ersten Laufrichtung. Die Methode eignet sich auch für eine Kombination von Papierelektrophorese mit Papierchromatographie.