Action of leucine aminopeptidase 1 and 2 (Aspergillus oryzae 410) on various synthetic peptides (author's transl)]

By use of di- or tripeptides as substrates, LAP 1 and LAP 2, 2 fractions from Aspergillus oryzae hydrolyze oligopeptides that possess leucine as N-terminal amino acid. LAP 1 fraction also hydrolyzes the histidyl bond. Both fractions have no activity towards peptides as glutathion, gly-pro-ala; they have low or no activity towards tyrosyl and tryptophanyl bond.     

Molecular weight of lipovitellins during egg development in the crustacean amphipod Orchestia gammarella (Pallas)]

Egg lipovitellins of Orchestia gammarella tested by electrophoresis on gels of different acrylamide concentrations, following the procedure of Hedrick and Smith (1968), display a migration pattern identical to that of proteins with molecular weights of congruent to 3.2 x 10(5) (lipovitellin I) and congruent to 5.5 x 10(5) (lipovitellin II) respectively. Since their molecular weight remains constant during embryogenesis, the changes of their relative mobility in disc gels indicates alterations in their ionic charges.     

Oxygen consumption in rat skeletal muscle at various rates of oxygen delivery

Summary Resting oxygen consumption of the vascularly isolated gracilis anticus muscle of the rat perfused by natural circulation via the femoral artery with diluted or undiluted blood depends on oxygen delivery (the product of flow rate and arterial oxygen concentration-DO₂) only when DO₂ falls below 16 l/min/g.     

Biochemical changes of muscle proteins in goldfish (Carassius auratus) during thermal acclimatization

Zusammenfassung Kälteadaptierte Goldfische (Carassius auratus) bauen mehr Leuzin in Muskelproteine ein, wobei die Steigerung in verschiedenen Muskeleiweissfraktionen gleich ist. Aus kälteadaptierten Goldfischmuskeln ist mehr Myosin und sarkoplasmisches Eiweiss extrahierbar.     

Phosphocreatine and ATP concentrations increase during flow-stimulated metabolism in a non-contracting muscle

The gracilis muscle was excised from cold-acclimated rats, placed in vitro, and simultaneously perfused via its artery by high pO₂ medium and superfused by low pO₂ medium. With a doubling of the perfusion rate (from 50 to 100 μl/min) phosphocreatine and ATP increased by 39% and 44%, respectively.     

Energetics of locomotion in a monotreme, the echidna Tachyglossus aculeatus.

The steady state oxygen consumption of two echidnas was measured during locomotion on a treadmill. The change in power input with change in velocity is similar to that found in other mammals, but the total energy requirement for locomotion is less. The significance of these findings in an animal with low basal metabolism and distally heavy limbs is discussed.     

Phosphocreatine and ATP concentrations increase during flow-stimulated metabolism in a non-contracting muscle.

The gracilis muscle was excised from cold-acclimated rats, placed in vitro, and simultaneously perfused via its artery by high pO2 medium and superfused by low pO2 medium. With a doubling of the perfusion rate (from 50 to 100 microliters/min) phosphocreatine and ATP increased by 39% and 44%, respectively.     

Coexistence of neoteny and an internal cycle a polystomatid (Monogenea)]

Metapolystoma cachani a parasite of Ptychadena longirostris (Peters) has been discovered in the Ivory coast. Its life cycle is the first to involve both the possibility of an internal cycle in the bladder of the adult Amphibian and a neotenical reproduction on the gills of the Tadpole. This double process is the link between the Monogeneans which are only parasites of aquatic hosts and those which are adapted to terrestrial hosts. Its place is therefore essential in the phylogeny of Polystomatidae.     

Observation of bacteriophages in wine (author's transl)]

Electron microscopic examination of samples of Swiss wine, collected during the malolactic fermentation, revealed the presence of bacteriophages of three different morphological types. It is interesting to note that these phages have been found in a product whose pH is lower than 3.5.     

Changes in the blood cortisol levels during the first week of life in calves (Bos taurus) and lambs (Ovis aries)]

At birth, the plasmatic level of cortisol is very variable and very high in the new-born Calf or the newborn Lamb. This level diminishes within one day and then varies between 10 and 30 ng/ml during the six following days. In the Calf, the secretion of cortisol can be directly stimulated by ACTH or inhibited at the level of the hypothalamic and hypophyseal system with dexamethasone on the day following birth.     

Synthesis of nuclear proteins incorporated into chromatin during anterior traumatic regeneration of an annelid (Owenia fusiformis)]

3H Trp and 14C Lys incorporation in proteins from chromatin of normal and regenerating animals, purified on metrizamide, show that the synthesis of these proteins is different in the two cases.     

Study of the mitotic activity during the preneoplastic period in the liver of rats treated by diethylnitrosamine (author's transl)]

The chronic administration of diethylnitrosamine in drinking water induces liver tumors in the rat. The preneoplastic period may be divided in two phases differing mainly in the loss of circadian control of mitotic activity in hepatocytes.     

Studies on stable and during early imbibition phases synthesized poly(A)-RNA of Agrostemma githago embryos (author's transl)]

RNA isolated from dry embryos of Agrostemma githago seeds contains poly(A)-sequences, but in very small amounts. In the early phase of imbibition, an intensive synthesis of poly(A)-containing RNA is brought about. The importance of this synthesis of poly(A)-RNA is discussed.     

Isolation of a pathogenic rhabdovirus of brown trout (Salmo trutta L., 1766)]

A Rhabdovirus pathogenic both for Rainbow and Brown Trout has been isolated from Brown Trout suffering heavy mortalities associated with muscular haemorrages and necrotic lesions of kidney and spleen. According to seroneutralization tests and host range, this virus appears to be different from the other known salmonids rhabdoviruses. Upon the basis of its in vitro gross characteristics, it is identical to Viral Haemorragic Septicemia Virus (VHS), F1 serotype, but differs from Infectious Hematopo?etic Necrosis and VHS type II viruses.