植物原生质体培养与遗传操作

一、植物原生质体培养和细胞融合的一些进展 1960年,E.C.Cocking用酶法从高等植物细胞中分离得原生质体,为高效地从高等植物中分离原生质体打开了局面。迄今已能从几乎所有的植物薄壁细胞中分离得生活的原生质体,并对种类日渐增多的植物原生质体进行培养。据不完全统计,迄今世上已能使近百种植物由原生质体培养成植株。在我国,除早年已由胡萝卜、菸草和矮牵牛等原生质体再生植株外,近年对园艺、药用、经济植物和禾谷类植物的十个科约24种植物,用原生质体培养物再生了植     

两种小麦的原生质体培养再生植株

报道两种基因型小麦昌乐5号和山农587胚性悬浮细胞系的建立和原生质体再生植株。小麦昌乐5号和山农587的胚性愈伤组织继代一年以后形成部分颗粒状和粉粒状结构,可用于悬浮培养。当分散好、生长快的胚性悬浮细胞系形成后,即可作为原生质体培养的材料。较长时间的悬浮培养容易使材料的胚性丧失,昌乐5号的胚性悬浮细胞系20天左右建成,其原生质体再生植株的频率为14/10~5(再生植株数/植板的原生质体数);而山农587的悬浮培养物需5个月以后才能用于原生质体培养,其原生质体再生植株的频率仅为4/10~6。     

木本植物原生质体培养与融合研究进展

为了全面了解模板植物原生质体培养和融合的现状，找出原生质体培养的规律，概述了原生质体的分离、培养及再生植株的条件和影响因素，介绍了国内外原生质体培养和融合及无性系变异筛选及遗传转化的研究动态。     

植物原生质体获得、培养条件及方法对其再生体系构建的影响

概述了植物原生质体再生体系中原生质体获得、培养条件及培养方法等关键技术环节,并且对完善植物原生质体再生体系及今后工作方向提出了建议.     

赤霉素产生菌~#218菌株的研究——Ⅰ.原生质体的分离和再生

赤霉素产生菌~#218菌株菌丝体用玻璃纸平板培养法培养,培养时间为36小时。采用2%蜗牛酶,不须硫醇类化合物预处理,可直接裂解赤霉素产生菌~#218菌株菌丝体,得到大量的原生质体。将原生质体悬浮液接种到高渗性再生培养基上,再生频率约为1～2%。原生质体再生菌株仍保留亲株的菌落形态及合成赤霉素的生产能力。     

水稻叶肉原生质体的分离与培养

研究了秧龄、纤维素酶浓度、纤维素酶 纤维素酶和果胶酶的比例等因素对分离水稻叶肉原生质体的影响,建立了培养水稻无菌苗、用叶鞘分离水稻叶肉原生质体的方法,该方法解决了愈伤组织诱导困难,分离水稻原生质体的分离期长、费时、费力的问题,大大缩短了分离原生质体的时间,对水稻叶肉原生质体的培养条件也进行了初步的研究。     

林木原生质体培养研究进展

本文概述了近年来林木原生质体培养的研究进展，郑重论述了不同树种和基因型、培养基、培养方法以及激素种类、浓度和配比对林木原生质体生长的影响，并针对林木原生质体培养中所存在的问题进行了较详细的讨论。     

植物细胞融合的研究进展(综述)

概述了原生质体分离和培养的影响因素，介绍了近年来国内外原生质体培养与融合及杂种细胞、筛选和鉴定的动态。     

大蕉胚性培养物及其原生质体再生植株的染色体分析

 采用看护培养法对大蕉胚性细胞悬浮系（Embryogenic cell suspensions, ECS）来源的原生质体进行培养,并研究了不同品种的看护细胞对原生质体培养的影响,以及大蕉ECS及其原生质体再生植株的染色体数目。结果发现,采用贡蕉ECS作为看护细胞时,大蕉原生质体培养15 d后细胞分裂频率达到8.5%,培养30 d后细胞团形成率为0.35%;而以大蕉ECS作为看护细胞时,全部原生质体褐化,不能正常发育。染色体检测结果表明,大蕉ECS的染色体数目不稳定,除含有正常的三倍体染色体数目的细胞（2n=3x=33）外,还出现大量染色体数目异常的细胞。其中绝大部分细胞含有多倍和非整倍的染色体数目,仅14.5%的细胞含正常三倍体染色体数目;但通过ECS原生质体培养获得的再生植株具有正常的三倍体染色体数目（2n=3x=33）。     

“赤豆叶肉细胞原生质体培养及绿苗分化”的研究居国际领先地位

我校遗传学研究所由葛扣麟教授、王蕴珠副教授领导的,以研究生为主体的国家攻关课题组,在籽粒用豆科植物原生质体培养方面,取得了重大突破性进展.他们首次从赤豆叶肉细胞原生质体游离培养成具有分化潜力的再生愈伤组织;并首次从再生愈伤组织培养分化成植株,从而表明了在国际上激烈的科技竞争中,在豆科粮食作物原生质体培养技     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with