Effects of several preoperative medications on fat cell lipolysis, and activity of adipose tissue cyclic AMP phosphodiesterase.

Effects were examined of atropine, diazepam, pethidene, promethazine, scopolamine, omnopon and papaverine on basal and noradrenaline-stimulated lipolysis in rat isolated fat cells and on rat adipose tissue cyclic AMP phosphodiesterase activity. Papaverine at high concentration (1 mM) inhibited both basal and hormone-stimulated lipolysis, whereas diazepam enhanced basal lipolysis. At a 'clinical dose', omnopon increased both basal and noradrenaline-stimulated lipolysis. Adipose tissue cAMP phosphodiesterase activity was strongly inhibited by 1 mM diazepam, papaverine, promethazine and omnopon (280 microgram ml-1). Lack of enhancement of lipolysis by the established cAMP phosphodiesterase antagonist papaverine, is compatible with simultaneous inhibition also of adipose adenyl cyclase. Diazepam-stimulated lipolysis is compatible with its phosphodiesterase inhibitory activity. It is proposed that papaverine-containing omnopon may offer some survival advantages during surgical stress by facilitating a caloric supply.     

Isoforms of baker's yeast transketolase

. .     

Do the respiratory muscles and lung receptors influence the respiratory sensitivity to CO2?

, CO₂. CO₂ CO₂ , . , CO₂, CO₂ . , , CO₂, CO₂ .     

The effect of chloramphenicol analogs on protein biosynthesis in a cell-freeEscherichia coli B system

a aaa a aa aa a a E. coli aa, a aa aa a , a a aa - a .

---

---

Laboratory of Biological Investigations.

---

Laboratory for Chemistry of Antibiotics.     

Synthesis and pharmacological properties of [1-N4-dimethyl-asparagine]-angiotensin II

Summary [1-N⁴-Dimethyl-asparagine]-angiotensin II was synthesized by Merrifield's solid-phase procedure. The analogue gave rat blood pressure about 70% relative potency to Hypertensin (Ciba). Rabbit aorta strips gave intrinsic activity _E=1, a PD₂ of 6.92±0.09 and an affinity relative to [Asn¹]-angiotensin II of 6.5%.The editors do not hold themselves responsible for the opinions expressed in the authors' brief reports. — Les auteurs sont seuls responsables des opinions exprimées dans ces brèves communications. — Für die Kurzmitteilungen ist ausschliesslich der Autor verantwortlich. — Per le brevi comunicazioni è responsabile solo l'autore. — . — Solo los autores son responsables de las opiniones expresadas en estas comunicationes breves.Acknowledgment. The authors wish to express their appreciation to Dr Emanuel Escher, School of Medicine, Centre Hospitalier Universitaire, Sherbrooke, Quebec, for his help in providing the biological data and critical comments.     

X-ray research for polycrystals. The formation geometry of instrumental width of the diffractional line at the investigation of the polycrystals in the translucent X-rays

: , , , - , ().     

Methylation of canine cardiac myosin in culture

Summary N, N, N, trimethyllysine and an unidentified methylated amino acid which co-electrophoresed and cochromatographed with the hydrolysis product of S-adenosyl-L-methionine, occur in fetal canine cardiac myosin and are isotopically labeled in vitro with S-adenosyl-L-(methyl³H) methionine between the 10th and the 12th day of culture.     

A new group of rifamycin derivatives displaying activity against rifampicin-resistant mutants ofStaphylococcus aureus

Zusammenfassung Unter einer grossen Anzahl semi-synthetischer Rifamycinderivate wurde eine Gruppe strukturell verwandter Verbindungen gefunden, die neben ihrer hohen Wirksamkeit (MIC 0,002-0,01 g/ml) gegenüber rifampicinsensiblen, grampositiven Keimen in vitro auch eine gute Wirkung (MIC 1–10 (g/ml) gegenüber rifampicinresistenten, grampositiven Keimen aufweisen. Die Resistenzrate grampositiver rifampicinresistenter und -sensibler Keime ist bezüglich dieser Verbindungen mindestens 100mal kleiner als bei Rifampicin. Die Wirkung gegenüber resistenten Keimen scheint nicht auf einer Beeinflussung der DNA-abhängigen RNA-Polymerase zu beruhen. Eine Struktur-Wirkungs-Beziehung wird angegeben.     

Chromosomes and giemsa-bands of the Idaho spotted ground squirrel,Spermophilus brunneus (Howell)

-Spermophilus brunneus . , 13 18 ,S. brunneus S. townsendii mollis (2n=38) , , ; ;S. brunneus . S. brunneus , Spermophilus.

---

---

Supported by National Science Foundation Grants No. GB32114X and No. GB 29131X, and the Sprague Foundation. We thankD. Pozin for technical assistance.     

The role of the pentose-phosphate pathway in adjustment of the heart to a high load and the development of myocardial hypertrophy

, , - - - . .     

Metabolism in Porifera XII. Further informations on the biosynthesis of 3β-hydroxymethyl-A-nor-steranes in the spongeAxinella verrucosa

Summary Direct incorporation and cold trap experiments suggest that cholest-4-en-3-one is an intermediate in the conversion of cholesterol into 3-hydroxymethyl-A-nor-cholestane in the marine spongeAxinella verrucosa. Cholest-4-en-3-one is further transformed by the sponge into cholest-4-en-3-ol, 5-cholestan-3-one and 5-cholestan-3-ol; these compounds arise from side reactions, which are not part of the major metabolic route leading to 3-hydroxymethyl-A-norsteranes.     

The synthesis of stereoselectively labelled porphobilinogen and its incorporation into protoporphyrin-IX

Summary 11-(R)-²H porphobilinogen, stereospecifically labelled with deuterium in the aminomethylene group has been incorporated into protoporphyrin-IX by haemolysates of chicken erythrocytes. High field NMR spectroscopy confirms that the overall biochemical process is stereospecific, deuterium being retained at the -, -and -meso positions and lost from the -meso position.27 August 1986     

Reaction of pyridoxal-5′-phosphate with γ-carboxyglutamic acid

Summary Pyridoxal-5-phosphate (PLP) reacts with -carboxyglutamic acid (Gla) to form a stable complex absorbing at 325 nm. It is suggested that a condensation occurs in which the formyl group of PLP reacts with the -amino group and the carbon atom of Gla to give a pyrrolidine derivative.     

Study of neurosecretory cells ofHelix pomatia by intracellular dye injection

#x043E; , . , .     

Neurogenic responses of resistance and capacitance vessels

- . , , , . , .     

Contribution to the method for approximation of X-ray diffraction line profile

. , . .     

A historical review of the problem of mitogenetic radiation

Summary The miracle of caryokinesis was the starting point that stimulated Alexander G. Gurwitsch to carry out his famous mitogenetic experiments in 1923. The results obtained confirmed his hypothesis of a weak radiation from cells, which is able to trigger the growth of other cells. Extensive experimental work within the first two decades after this discovery indicated that the problem of mitogenetic radiations is generally related to the biological significance of UV-radiation. Both energetic and informational aspects have to be considered, namely radiation effective in activating molecules, and that involved in arranging them into larger units.The molecular organization of biological structures is evidently governed by nonequilibrium conditions needing the uptake or emission of radiation. These concepts of A. G. Gurwitsch can be linked with modern approaches based on hypotheses of coherence in biology, synergetics and dissipative structures. However, the question of causal interrelationships between this part of non-equilibrium radiation and biological matter on different levels of evolution has to be solved now.     

Changes in the lumen of coronary vessels under oligemic hypotension

Conclusions The decrease of circulating blood volume, resulting in oligemic hypotension, may evoke in anaesthetized cat the active constrictory coronary vessel responses of 2 types, one occuring immediately after start of the hypotension and the other having a significantly prolonged latency.

---

, , , , ( 4 ), ( 45 ).

     

Interaction of polyene antibiotics and serum lipoproteins

-.     

Cooperation between the two myosin heads interacting with actin in presence of ADP in myofibrils

Zusammenfassung Alkylierung von 2 Thiolgruppen pro Myosin mit NEM bei 0°C in Gegenwart einer Diophosphatkette inaktiviert die K-ATPase vollständig.In Myofibrillen werden diese Thiolgruppen durch Rigor-interaction beider Myosinköpfchen mit Actin vor Alkylierung geschützt. In Gegenwart von Mg-ADP tritt eine spezifische vom Rigor verschiedene interaction zwischen Myosin und Actin auf. Man muss annehemen, dass dabei nur 1 Myosinköpfchen ans Actin bindet und dass das andere Köpfchen ein ADP gebunden hat.