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1.
对分离到的两株硅酸盐细菌的解钾能力进行了测定,其中BM-BD菌株具有较强的解钾能力,接种该菌的摇瓶培养液中,水溶性钾含量为5.6 μg/mL,比不接菌对照增加了47.4 %,大于解钾模式菌株ACCC10013的解钾能力.对BM-BD菌株进一步试验表明该菌株在发酵罐培养条件下也能将云母中的矿物钾释放出来,接种培养108 h后,培养液中的水溶性钾增加了34%.  相似文献   

2.
采集生活污水,从中分离出具有解有机磷能力的菌株,纯化得到14株解有机磷细菌。通过平板法测定溶磷圈后,筛选出4株解磷能力较强的菌株进一步做解磷能力的定量测定。结果发现,OPB8、OPB17、OPB15、OPB19菌株达到解磷峰值时,解磷率分别为107.9%、135.1%、31.8%和249.2%,随着培养时间的延长,培养液中的水溶性磷含量均有下降,其中OPB15、OPB19菌株的培养液中的水溶性磷含量分别降低了72.2%和95.7%。  相似文献   

3.
硅酸盐细菌JF88菌选育的研究   总被引:3,自引:0,他引:3  
笔者分离到硅酸盐细菌JF88菌株,经鉴定为邻单胞菌属,用四苯硼钠比浊法对该萝株的解钾能力进行测定,结果为实验组水溶性钾含量比对照组增加30.5%,用磷钼比色法对该菌的解磷能力进行测定,结果为JF88菌株的解磷能力是美国菌肥商品生产菌种MB1的15.79倍,用直接记数法测得JF88菌株的生长繁殖速度超过MB1。  相似文献   

4.
土钵培养法筛选硅酸盐细菌   总被引:1,自引:0,他引:1  
30种硅酸盐细菌菌株用土钵培养法进行筛选,分别在培养30、60、90、180d用火焰光度计测定土壤中钾离子含量;在所有研究的30种菌株中,菌株新6在土钵培养60d后检测出最高的土壤有效钾净增率22.1%;菌株F1具有最高的总土壤有效钾净增率20%;对土壤总有效钾含量进行统计学分析,筛选出14种菌株具有显著的解钾能力。  相似文献   

5.
太子参土壤固氮菌与解钾菌的分离、筛选及鉴定   总被引:1,自引:0,他引:1  
应用Ashby培养基和亚历山大硅酸盐细菌培养基从太子参根际土壤中分离固氮菌和解钾菌,并选取固氮菌和解钾菌进行固氮、解钾作用研究,以为太子参生物菌肥的研制提供菌源.结果表明:从太子参根际土壤中获得太子参自生固氮菌34株,解钾菌26株.选取固氮菌和解钾菌各5株,分别测定其固氮效能和解钾能力,其中以自生固氮菌ATS-2-5的固氮效能最高,达21.51mg/g;解钾能力最好的菌株为KTS-2-4,液体培养7d后水溶性钾含量为7.42mg/L.菌株培养特征、菌体形态观察、菌株生理生化反应测试并结合16SrDNA分析鉴定,将菌株ATS-2-5和KTS-2-4归属为同一个种,即栗树类芽孢杆菌(Paenibacillus castaneae).这是首次报道该菌株具有固氮、解钾作用的研究文献.  相似文献   

6.
多功能植病生防木霉的分离筛选与鉴定   总被引:1,自引:0,他引:1  
从采集的120份土壤样品中分离获得木霉244株。对峙培养发现其中对棉花立枯丝核菌(Rhizoctonia solani)具有拮抗能力的有114株;固体培养检测木霉的解磷和固氮能力,发现所有菌株都能够水解植酸钙,有74株能够水解磷酸钙,未发现具有固氮能力的菌株。对抑菌圈和解磷圈最大的19株木霉在液体培养条件下检测了解磷和解钾能力,其中菌株T13—8(1.792μg/mL)、123(1.652μg/mL)显示出较高的解磷能力,部分菌株在液体培养条件下不能解磷;部分菌株在液体培养下显示出解钾功能,其中菌株T47-4(20.585μg/mL)显示出较高的解钾能力。上述19个菌株的形态学和ITS序列分析鉴定表明,其中9个菌株为Trichoderma longibrachiatum,8个菌株为rharzianum,另外2个菌株分别为rvirens和zcitrinoviride。  相似文献   

7.
研究了碳源、氮源、接种量、培养时间、培养温度、培养基初始pH等条件对产中性纤维素酶真菌菌株瓶霉菌(Phialophora sp.z8)产酶的影响.结果表明:该菌株产中性纤维素酶的适宜碳源为1%稻草粉加1%可溶性淀粉,最适氮源为0.2%的(NH4)2SO4加0.1%的牛肉膏,接种量为5%,培养时间为72h,培养温度为28℃,培养基初始pH为6~7.在最适宜的条件下,培养液中内切葡聚糖酶活力可达到2.95IU/mL,而天然纤维素酶活力和滤纸酶活力却很低。  相似文献   

8.
用含有不同浓度(0、2.55、30.60mmol/L)钾离子的Hoagladn培养液培养的小麦接种叶锈病病原菌,接菌9d后统计接菌叶片上的孢子堆数目,结果表明钾能明显减轻小麦锈病的发生;小麦感染锈病后,叶中多酚氧化酶、过氧化物酶、苯丙氨酸解氨酶和超氧化物歧化酶的活性明显升高,用含较高浓度钾离子(30.60mmol/L)的培养液培养的小麦叶片酶活性的增加值显然高于用含较低浓度钾离子(2.55mmol  相似文献   

9.
6种乳酸菌降解胆固醇的体外试验   总被引:3,自引:0,他引:3  
分析了6种安全的乳酸菌菌种在不同条件下接种于体外高胆固醇的MRS-CHOL培养基中降解胆固醇的情况,以及培养液的pH值与胆固醇降解率的关系。结果表明,所用的6个菌种均可有效降解胆固醇,依次为Lp菌(28.088%)、3群菌(25.059%)、4群菌(24.922%)、Lf菌(24.554%)、Ld菌(22.248%)和5#菌(18.513%),降解率随接种量的增加和培养时间的延长而增加,除Lf菌外,其它菌株都随胆固醇浓度的变化而出现先升后降的趋势,培养液的pH值与胆固醇的降解率呈负相关性,且都不显著。  相似文献   

10.
硅酸盐菌剂的生产工艺及其性能研究   总被引:1,自引:0,他引:1  
本实验通过对不同培养条件下钾细菌生长数量的测定,确定了硅酸盐菌剂生产工艺。此外,以化学分析和生物测定的方法,对菌种性能测定的结果表明,硅酸盐菌剂确具利用和分解硅酸盐和磷矿粉的能力,水溶性磷含量比对照增加84×230%,水溶性钾含量比对照增加38×52%  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
王慧 《科技信息》2008,(10):240-240
Wuthering Heights, Emily Bronte's only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.  相似文献   

14.
The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.  相似文献   

15.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

16.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

17.
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-  相似文献   

18.
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-  相似文献   

19.
正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that  相似文献   

20.
<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with  相似文献   

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