Certain biochemical effects of garlic oil on rats maintained on high fat-high cholesterol diet

Summary The feeding of a high fat-high cholesterol (HF-HC) diet to normal rats for 1 month increased the lipid components cholesterol and triglyceride in serum, liver and kidneys and decreased the serum albumin very significantly. Administration of garlic oil (100 mg/kg b. wt/day) for 1 month together with the HF-HC diet to another group almost nullified the lipid-increasing and albumin-decreasing effects of that diet. The reduction in total lipids, cholesterol and triglycerides and the restoration to normal level of serum albumin were highly significant in the garlic oil group. Adipose tissue triglyceride lipase activity was significantly increased in both the above groups with a much greater rise in the oil group.     

Hypolipidemic effects of garlic oil in rats fed ethanol and a high lipid diet

Summary Feeding of ethanol and a high fat-high cholesterol diet to rats markedly increased the total lipids in the liver, and cholesterol and triglyceride levels in the serum, liver and kidneys. However, when ethanol mixed with 0.5% garlic oil was fed to animals maintained on the high fat-high cholesterol diet, these lipid levels were significantly reduced to levels near to those seen in untreated control rats. Garlic oil did not reduce the serum albumin or the total proteins of liver, kidneys or serum when fed along with ethanol. Probably the garlic oil enhances the catabolism of dietary cholesterol and fatty acids.The authors acknowledge with thanks the financial assistance of the University of Maiduguri for carrying out this project.     

Renal effects of a high unsaturated fat diet in renal artery stenosis in rats

The renal effects of an unsaturated fat (UNSAT) diet in mild to moderate two-kidney, one-clip (2K1C) renovascular hypertension were evaluated. An UNSAT diet (37% by energy) prevented the development of hypertension compared to 2K1C rats fed a high saturated fat (SAT) (37% by energy) and a normal fat (CONTROL) (11% by energy) diet. Urinary sodium and fractional sodium excretion increased in 2K1C rats as compared to SHAM operated controls, regardless of the diet received. In the early weeks of the experiment (weeks 2–4 post-surgery to induce hypertension), an enhanced natriuresis occurred in the 2K1C UNSAT as compared to the 2K1C CONTROL and SAT diet groups. This resulted from an increase in the glomerular filtration rate (GFR in mls·min^–1) as measured using the single-injection [⁵¹Cr] EDTA method (2K1C UNSAT; 1.99±0.18 versus 2K1C SAT; 1.27±0.09, p<0.02; and versus SHAM CONTROL; 1.45×0.01; p<0.02). The increased GFR was not associated with alterations in effective renal plasma flow (ERPF) as measured using the single-injection [¹²⁵I] Na hippurate method. No differences in sodium excretion; GFR; ERPF or renal blood flow (microsphere technique) were noted between the 2K1C UNSAT and SAT diet groups at weeks 6–8 post-surgery, despite a continued antihypertensive effect of the UNSAT diet. Hence, the antihypertensive effect of an unsaturated fat diet in 2K1C renovascular hypertension in rats is associated with transient glomerular changes leading to an enhanced natriuresis.     

Physiological changes in hairless mice maintained on an antioxidant supplemented diet

Summary Several physiological parameters were measured in hairless mice maintained on a diet supplemented with antioxidants. Comparisons to animals on control diet revealed higher water-soluble antioxidant content of skin and increased liver weight. Only small differences in body weight occurred and no distinct histological changes were observed in skin or liver.This investigation was supported by National Research Service Award 1 F32 CAO5062, awarded by the National Cancer Institute, and USPHS Grant CA13464-04 from the NCI.     

Substance P and neurotensin: Opposite effects on plasma cholesterol levels in ovariectomized conscious rats

Summary Intravenous pulse injection of neurotensin produces a significant and dose-dependent increase in plasma cholesterol levels in ovariectomized conscious rats, whereas substance P has opposite effect. 4-Aminopyrazolo (3,4,-d) pyrimidine, (4-APP) also significantly lowers plasma cholesterol. The suppressive effects of both substance P and 4-APP were readily reversed by neurotensin, suggesting that the peptides act on hepatic lipoprotein secretion.Supported by grants from the Indian Council of Medical Research (ICMR), New Delhi. K.R. was recipient of a UGC fellowship.     

Collagen proteins in rats on long-term tyrosine diet

Zusammenfassung Bei Ratten wurde nach 12monatlicher Diät mit 5% 1-Tyrosin eine Alkaptonurie hervorgerufen. Die histologische Prüfung ergab eine Übersättigung mit der Homogentisinsäure, eine verringerte Aktivität der Oxidase der Homogentisinsäure in der Leber und deren massive Ausscheidung im Harn.     

Surface morphology of the subfornical organ: effects of low and high sodium chloride diet

Ependymal cells found in the subfornical organ of the rat were counted. Cells covered by small microvilli, small protrusions and smooth cells were frequently found. Also present were cells with long or short cilia, cels with large protrusions and supraependymal cells. High and low sodium diets reduced the number of cells with large protrusions. Microvilli-covered cells increased after a low sodium diet.     

Morphological and biochemical effects of ethylene on Tulips

Summary Tulip bulbs treated with ethylene at various concentrations, at different stages of development and during various lengths of time, exhibited various morphological abnormalities: open bud, stamen blasting, abnormal number of floral parts, increased number of second-year bulbs. These morphological changes were paralleled by rapid variations in the contents of proteins and RNAs, and a slow, yet noticeable, increase of the DNA content of flower buds.     

Plasma lipid concentration and lipoprotein distribution in exercising and nonexercising rats fed a high sucrose diet

Summary Voluntary physical activity in rats fed a high sucrose diet reduces the plasma triglyceride level but has no major influence on the lipoprotein distribution.Acknowledgment. The excellent technical assistance of Ida Goffeng Bay is gratefully acknowledged.     

Effect of high lipid diet on circadian rhythms of tissue glycogen

Summary The well defined circadian rhythms of glycogen content in heart, diaphragm and liver of the rat are drastically altered by a high lipid diet as shown by changes in amplitude, phase and tissue glycogen levels. If sampling times had been restricted to certain hours of the day the profound effect of the high fat diet on tissue glycogen would not have been apparent.Supported by USPHS grants HL 16041-03 and HL 07094-03.     

The effect of skim milk on plasma cholesterol in rats.

From the 15th day of birth, newborn rats were offered rat chow mixed with water or skim milk. The former also received tap water, the latter skim milk. In the latter group, plasma cholesterol was lowered in 43- and 64-day-old males and in 64-day-old females.     

Studies on the biochemical effects of glibenclamide on alloxan diabetic rabbits

Treatment of alloxan diabetic rabbits with glibenclamide, the most potent of the sulfonylureas, for a period of 2 months, significantly ameliorated the diabetic condition. It produced a decrease in serum and liver lipids, amino acids, serum urea, blood sugar and urine sugar; increase in body weight, serum and liver proteins, liver glycogen, glucose tolerance and serum and liver acid labile phosphates. The possible mechanism of action of this hypoglycemic agent is discussed.     

Studies on the biochemical effects of glibenclamide on alloxan diabetic rabbits

Summary Treatment of alloxan diabetic rabbits with glibenclamide, the most potent of the sulfonylureas, for a period of 2 months, significantly ameliorated the diabetic condition. It produced a decrease in serum and liver lipids, amino acids, serum urea, blood sugar and urine sugar; increase in body weight, serum and liver proteins, liver glycogen, glucose tolerance and serum and liver acid labile phosphates. The possible mechanism of action of this hypoglycemic agent is discussed.     

Certain biochemical changes in the ovary of a migratory bird,Calandrella acutirostris tibetana

Summary The changes in the ovarian cholesterol, glycogen and ascorbic acid of Bagheri,Calandrella acutirostris tibetana, during its non-breeding and breeding periods were studied. There was a significant increase in all the 3 biochemical constituents during the breeding period.     

Certain biochemical changes in the ovary of a migratory bird, Calandrella acutirostris tibetana.

The changes in the ovarian cholesterol, glycogen and ascorbic acid of Bagheri, Calandrella acutirostris tibetana, during its non-breeding and breeding periods were studied. There was a significant increase in all the 3 biochemical constituents during the breeding period.