Palaeoenvironmental changes from pollen record in deep sea core PC-1 from northern Okinawa Trough, East China Sea during the past 24 ka

A pollen record of core PC-1 from the northern Okinawa Trough, East China Sea （ECS）, provides information on vegetation and climate changes since 24 cal. kaBP. A total of 103 samples were palynologically analyzed at 8 cm intervals with a time resolution of 230 a. Four pollen zones are recognized： zone Ⅰ （812-715 cm, 24.2-21.1 cah kaBP）, zone Ⅱ （715-451 cm, 21.1-15.2 cah kaBP）, zone Ⅲ （451-251 cm, 15.2-0.8 cah kaBP）, zone Ⅳ （251-0 cm, 10.8-0.3 cal. kaBP）, corresponding to Late MIS 3, Last Glacial Maximum （LGM）, deglaciation and Holocene, respectively. The LGM is characterized by the dominance of herbs, mainly Artemisia, and high pollen influx, implying an open vegetation on the ex- posed continental shelf and a cool and dry climate. The deglaciation is a climate warming stage with Pinus percentage increased and Artemisia percentage decreased and a rapid sea-level rise. The Holocene is characterized by predominance of tree pollen with rapid increase in Castanea-Castanopsis indicating the development of mixed evergreen and deciduous broad-leaved forest and a warm, humid climate. Low pollen influx during the Holocene probably implies submergence of the continental shelf and retreat of the pollen source area. The vegetation indicated by pollen assemblage found in this upper zone is consistent with the present vegetation found in Kyushu, Japan. Originating from the humid mountain area of North Luzon of the Philippines, Tasmania and New Zealand, Phyllocladus with sporadic occurrence throughout PC-1 core probably suggests the influence of Palaeo-Kuroshio Current or intense summer monsoon. The observed changes in Pinus and Herbs percentage indicate fluctuations of the sea level, and high Pinus percentage corresponds to high sea level. Spectrum analysis of the pollen percentage record reveals many millennial-scale periodicities, such as periodicities of 6.8, 3.8, 2.2, 1.6 ka.     

Millennial-scale paleoceanography in Okinawa Trough during Late Quaternary period

Based on the analyses of foraminifer and accelerator mass spectrometer radiocarbon dating in DGKS9603 core from mid-Okinawa Trough close to bottom, oscillation curve, which expressed the relation between the surface water temperature and the depth, has been obtained by using foraminifer analysis and calculation of FP-12E transfer function. The whole core indicated seven cold phases and eight warm phases. Obvious expression of low temperature event during Middle and Late Holocene, YD,H1,H2,H3 and H4 events, as well as the short cold phase during the middle last glacial period, implied that short shifts since 50 kaBP would have been global significance. Sedimentation rate during cold phases is usually faster than that in warm stages, with the lowest rate in Holocene, which may be connected with rising sea level and principal axial of Kuroshio Current moving to west. Volcanic activities highly developed in Okinawa Trough during the Quaternary period, thus abundant volcanic glass and pumice were well preserved.     

Paleoenvironmental information recorded by pollen in B-3GC gravity core in Okinawa Trough

Based on pollen records in B-3GC gravity core, environmental change since 9500 aBP of Okinawa Trough and its adjacent islands was derived. The result showed that the most time during this period was in a warm temperate climate except in middle Holocene (6800-4400 aBP) that was under subtropical climate control. During 9500–8300 aBP and 3100–2000 aBP periods, it appeared colder and drier than the rest time. The original area of pollen sources surrounding Okinawa Trough was covered by evergreen and deciduous broadleaf forest alternatively with mixed broad-leaf-conifer forest distributed in high mountainous areas. Usually, these three kinds of forests existed at the same time with difference in altitude. Pollen from subtropical and tropical plants increased obviously in about 5000–6000 aBP, reflecting a great lifting of vegetation zone and expansion of evergreen broad-leaf forest in the study area. However, there was a slight descending of plant zone and shrinking of evergreen broadleaf forest during 9500-8300 aBP and 3000-2000 aBP. During the remaining periods vegetation zone was higher than the present but in a limited range.     

Reduced nitrogen fixation in the glacial ocean inferred from changes in marine nitrogen and phosphorus inventories.

To explain the lower atmospheric CO2 concentrations during glacial periods, it has been suggested that the productivity of marine phytoplankton was stimulated by an increased flux of iron-bearing dust to the oceans. One component of this theory is that iron-an essential element/nutrient for nitrogen-fixing organisms-will increase the rate of marine nitrogen fixation, fuelling the growth of other marine phytoplankton and increasing CO2 uptake. Here we present data that questions this hypothesis. From a sediment core off the northwestern continental margin of Mexico, we show that denitrification and phosphorite formation-processes that occur in oxygen-deficient upwelling regions, removing respectively nitrogen and phosphorus from the ocean-declined in glacial periods, thus increasing marine inventories of nitrogen and phosphorus. But increases in phosphorus were smaller and less rapid, leading to increased N/P ratios in the oceans. Acknowledging that phytoplankton require nitrogen and phosphorus in constant proportions, the Redfield ratio, and that N/P ratios greater than the Redfield ratio are likely to suppress nitrogen fixation, we suggest therefore that marine productivity did not increase in glacial periods in response to either increased nutrient inventories or greater iron supply.     

Iron and phosphorus co-limit nitrogen fixation in the eastern tropical North Atlantic

The role of iron in enhancing phytoplankton productivity in high nutrient, low chlorophyll oceanic regions was demonstrated first through iron-addition bioassay experiments and subsequently confirmed by large-scale iron fertilization experiments. Iron supply has been hypothesized to limit nitrogen fixation and hence oceanic primary productivity on geological timescales, providing an alternative to phosphorus as the ultimate limiting nutrient. Oceanographic observations have been interpreted both to confirm and refute this hypothesis, but direct experimental evidence is lacking. We conducted experiments to test this hypothesis during the Meteor 55 cruise to the tropical North Atlantic. This region is rich in diazotrophs and strongly impacted by Saharan dust input. Here we show that community primary productivity was nitrogen-limited, and that nitrogen fixation was co-limited by iron and phosphorus. Saharan dust addition stimulated nitrogen fixation, presumably by supplying both iron and phosphorus. Our results support the hypothesis that aeolian mineral dust deposition promotes nitrogen fixation in the eastern tropical North Atlantic.     

Shifts of the Kuroshio axis over the last 20 000 years

Core 255 from the southern Okinawa Trough was selected to monitor the shift of the Kuroshio axis over the last 20 000 years. During the last glacial maximum the Kuroshio axis had ever shifted outside the Okinawa Trough. It entered the Okinawa Trough again at about 6 500 a BP, leading to abrupt increase of sedimentation rate, distinctly coarser sediment and remarkably increased abundance of foraminiferal indicators of the Kuroshio in the core. But, during about 4 000-3 000 a BP the abundance of the Kuroshio indicatorPulleniatina obliquiloculata sharply decreased again, indicating that the Kuroshio axis had ever shifted casterly for a short period or the Kuroshio was weakened and that moment.     

Reconstructing sea surface temperature,sea surface salinity and partial pressure of carbon dioxide in atmosphere in the Okinawa Trough during the Holocene and their paleoclimatic implications

The sediment core DGKS9603 collected from the Okinawa Trough was used as research target. By use of unsaturated indexU ^k₃₇ of long-chain alkenone, δ¹³C of POC and of planktonic foraminifera (G. sacculifer), the evolutions of sea surface temperature and partial pressure of carbon dioxide in the atmosphere during the Holocene were reconstructed in the Okinawa Trough. And in combination of δ¹⁸O of planktonic foraminifera, the relative difference of sea surface salinity during the Holocene was also reconstructed. Consequently, three cooling events (E₁-E₃) were identified, each of which occurred at 1.7-1.6, 5.1-4.8 and 8.1-7.4 kaBP (cal), respectively. Of the three events, E₂ and E₃ are globally comparable, their occurrence mechanism would be that the main stream of the Kuroshio Current shifted eastward due to the enhanced circulation of the northeastern Pacific Ocean, which was driven in turn by amplified intensity of sunshine and subsequent enhancement of subtropical high pressure; E₁ corresponds to the Small Ice-Age Event occurring between 1550 and 1850AD in China. In the Oki-nawa Trough, E₁ might be also related to the eastward shift of main stream of the Kuroshio current driven by powerful Asia winter monsoon.

     

Factors affecting the abundance and community structureof the phytoplankton in northern South China Sea in the summerof 2008： a biomarker study

Phytoplankton biomarkers were analyzedusing suspended particles collected from the northernSouth China Sea （SCS） during the summer cruise of2008, with the goal of understanding the algal communitystructure and biomass distribution pattern in the summerseason. The results indicated that the distribution of algalbiomarkers in surface water of SCS was impacted andconstrained by the local hydrological settings： the highbiological community generally appeared in Pearl Riverestuaries, southwestern off Taiwan island and southeast-em off Hainan Island, while the relatively lower biomasswas found in the deep basin and strait areas. Diatomswere the dominant phytoplankton species, which werefollowed by dinoflagellates. Coccolithophorid biomassgradually increased toward the open ocean. The presentwork indicated that the algal biomarkers effectively doc-umented the variability of the phytoplankton biomass andcommunity structure as well as their linkage with theoceanic dynamics in SCS during summer 2008. Thisresearch provided not only the foundation for the appli-cation of algal lipids in the modem ocean ecosystem, butalso the basis for the reconstruction of the past oceanicalgal community structures.     

Seasonal variation of size-fractionated phytoplankton in the Pearl River estuary

To investigate the dynamics of phytoplankton size structure in the Pearl River estuary, concentrations of size-fractionated chlorophyll a (Chl a) were determined during four cruises carried out in 2008 and 2010. The distribution of Chl a in this geographical location showed a high degree of temporal variation. Chl a concentrations were highest in autumn, approximately three times higher than those in summer and winter. Microphytoplankton was the dominant contributor, accounting for 66.9% of the Chl a concentration in autumn 2008. In summer and spring 2008, nano-sized cells dominated the phytoplankton population throughout the study region. During the winter cruise, two different areas of water were found, characterized by (1) low salinity and high nutrient content and (2) high salinity and low nutrient content; nano- and picophytoplankton co-dominated the first area, while microphytoplankton dominated the second. It is arguable that grazing could have played a role in determining phytoplankton community size structure in winter. Nutrient concentrations were assumed not to limit phytoplankton growth during the investigation period. Size-differential capacity in competing for the resources available under different hydrodynamic conditions seemed to be the major factor in determining seasonal variation in the structure of the phytoplankton communities. High N:P ratios in the Pearl River estuary had major implications for nutrient pollution control. Our results indicated that studies of phytoplankton size structure provide greater insight into phytoplankton dynamics and are necessary to better manage water quality in the Pearl River estuary.     

Geological records of marine environmental changes in the southern Okinawa Trough

Indexes of sediment grain size,sedimentation rates,geochemical composition,heavy,minerals,benthic foraminiferal fauna,indicator species of the Kuroshio Current,paleo-SST and carbonate dissolution of core E017 conformably suggest a great marine environmental change occurring at about 10.1-9.2cal.kaBP in the southern Okinawa Trough,which may correspond to the strengthening of the Kuroshio Warm Current and re-entering the Okinawa Trough through the sea area off northeast Taiwan.The invasion of Kuroshio current has experienced a process of gradual strengthening and then weakening,and its intensity became more fluctuation during the last 5000 years.Compared to the transition of sediment grain size,geochemical composition and heavy minerals,the foraminiferal faunas show a 900-year lag,which may indicate that the invasion of Kuroshio Current and the consequent sea surface and deep-water environmental chanages is a gradual process,and fauna has an obvious lag compared to environment altering.The carbonate dissolution of the Okinawa Trough has had an apparent strengthening since 9.2cal.kaBP,and reached a maximum in the late 3000 years,which may be caued by the deep-water environmental changes due to the invasion of Kuroshio Current.     

Quaternary high-resolution opal record and its paleoproductivity implication at ODP Site 1143,southern South China Sea

The correlation of opal content and MAR with oxygen isotopic records of benthonic foraminifera at Site 1143, southern South China Sea indicates that,since about 900 ka,the increasing opal content and MAR during the interglacial periods is inferred to reflect the bigher surface productivity ,for the intensified summer monsoon during the interglacial periods would result in the enhanced upwelling and nutrient supply,Time-sequence spectral analyses of oxygen isotopic record ,opal content and MAR at intervals of 0-900 ka reveal that the changes of surface productivity were dominantly forced by the variations of the earth orbital cycles.     

Evidence against dust-mediated control of glacial-interglacial changes in atmospheric CO2

The low concentration of atmospheric CO2 inferred to have been present during glacial periods is thought to have been partly caused by an increased supply of iron-bearing dust to the ocean surface. This is supported by a recent model that attributes half of the CO2 reduction during past glacial stages to iron-stimulated uptake of CO2 by phytoplankton in the Southern Ocean. But atmospheric dust fluxes to the Southern Ocean, even in glacial periods, are thought to be relatively low and therefore it has been proposed that Southern Ocean productivity might be influenced by iron deposited elsewhere-for example, in the Northern Hemisphere-which is then transported south via ocean circulation (similar to the distal supply of iron to the equatorial Pacific Ocean). Here we examine the timing of dust fluxes to the North Atlantic Ocean, in relation to climate records from the Vostok ice core in Antarctica around the time of the penultimate deglaciation (about 130 kyr ago). Two main dust peaks occurred 155 kyr and 130 kyr ago, but neither was associated with the CO2 rise recorded in the Vostok ice core. This mismatch, together with the low dust flux supplied to the Southern Ocean, suggests that dust-mediated iron fertilization of the Southern Ocean did not significantly influence atmospheric CO2 at the termination of the penultimate glaciation.     

Compositions of aliphatic des-A-triterpenes in the Hani peat deposit,Northeast China and its biological significance

Aliphatic des-A-triterpenes, the degradation products of pentacyclic triterpenes have been tentatively assigned in samples taken from a 963-cm-thick peat sequence from the Hani region of Northeast China using gas chromatography-mass spectrometry (GC-MS) in order to understand and identify these des-A-triterpenes response to climate and ecology. The study indicates that aliphatic des-A-triterpenes are abundant in the Hani peat bog. The high relative abundance of the total des-A-triterpenes mainly occurs at the Holocene periods and is corresponding to the low sedimentation rate. The mechanism of des-A-triterpenes accumulation is not consistent with the previous studies. It is proposed that the biological community is also one influence factor. The obvious stage variations of the total des-A-triterpenes in the whole sequence reveal the different biological (microbes and plants) processes and responses, possibly revealing the four large replacements of biological community and functional composition in the swamp environment over the 16000 cal a BP. The low total des-A-triterpenes values occur at about 10200, 8700, 7700, 6600, 4480–3940 and 3040 cal a BP, reflecting the multiple millennial micro-environment oscillations under the macro-environment of the swamp during the Holocene. The ratios of diene and monoene des-A-triterpenes (DT/MT), des-A-lupane and diene and monoene des-A-triterpenes (DL/(DT+MT)), ratios of individual compounds exhibit vertical opposite to the total des-A-triterpenes. Namely, these ratios are high during the last deglaciation and late Holocene whereas they are low at other periods, indicating different compounds have different responses to micro-environment and microbial activities. This study discusses the diversity of biological community and biological functional composition response to micro-environment, which not only provides the complement in Northeast China, but also bears important fundamental information for the implication to ecological environment in the future.     

Magnetic properties of different grain-sized particles of sediments from the Okinawa Trough and their relationships to sedimentary environment

Multiple magnetic parameters were measured for nine different grain-sized fractions separated from the sediment samples that are representatives of four different sedimentary environments of the Okinawa Trough. Based on the measured results, the contributions of different grain-sized particles to total magnetic susceptibility of bulk sediments, the magnetic mineral assemblage and magnetic domain state as well as their relationships to sedimentary environment were discussed. Our research shows that the magnetic mineral is dominated by magnetite with a small amount of hematite and is primarily in pseudo-single-domain state. That indicates that the different sedimentary environments in the Okinawa Trough have certain correlation in material provenance. The magnetic minerals enrich in different grain-sized particles in response to different sedimentary environments. The contribution of the grain sizes from coarse to fine to coarse and fine to the magnetic susceptibility from the west to the east is in accordance with terrigenous material transportation from continental shelf of the East China Sea to the Okinawa Trough. It also shows difference in magnetic properties as a result of some environmental factors.     

Agricultural runoff fuels large phytoplankton blooms in vulnerable areas of the ocean

Biological productivity in most of the world's oceans is controlled by the supply of nutrients to surface waters. The relative balance between supply and removal of nutrients--including nitrogen, iron and phosphorus--determines which nutrient limits phytoplankton growth. Although nitrogen limits productivity in much of the ocean, large portions of the tropics and subtropics are defined by extreme nitrogen depletion. In these regions, microbial denitrification removes biologically available forms of nitrogen from the water column, producing substantial deficits relative to other nutrients. Here we demonstrate that nitrogen-deficient areas of the tropical and subtropical oceans are acutely vulnerable to nitrogen pollution. Despite naturally high nutrient concentrations and productivity, nitrogen-rich agricultural runoff fuels large (54-577 km2) phytoplankton blooms in the Gulf of California. Runoff exerts a strong and consistent influence on biological processes, in 80% of cases stimulating blooms within days of fertilization and irrigation of agricultural fields. We project that by the year 2050, 27-59% of all nitrogen fertilizer will be applied in developing regions located upstream of nitrogen-deficient marine ecosystems. Our findings highlight the present and future vulnerability of these ecosystems to agricultural runoff.     

The paleoclimatic events and cause in the Okinawa Trough during 50 kaBP

Planktonic foraminiferal δ ¹⁸O record for core DGKS9603 from the Okinawa Trough shows a series of climatic fluctuations and sudden cooling events in short time scale during 50 kaBP, which appear to correlate closely to the Younger Dryas and Heinrich events H1-5 recorded in Chinese loess, the South China Sea, the North Atlantic cores and the Greenland ice cores. Three polarity reversal events, correlating to Gothenburg, Mungo and Laschamp events, approximately correspond to Heinrich events H1, H3 and H5 respectively, which could be a cause of global climate changes. The δ ¹⁸O curve of the Okinawa Trough is well associated with the grain size record of the Lijiayuan loess profile in northwestern China and is somewhat different from the climate fluctuations documented in the Greenland ice cores. These correlation results indicate that regional factors play an important role in controlling the climate changes in the East Asia, and the East Asian Monsoon could be the prominent regional controlling factor.     

Unsaturated fatty acid content in seston and tropho-dynamic coupling in lakes

Determining the factors that control food web interactions is a key issue in ecology. The empirical relationship between nutrient loading (total phosphorus) and phytoplankton standing stock (chlorophyll a) in lakes was described about 30 years ago and is central for managing surface water quality. The efficiency with which biomass and energy are transferred through the food web and sustain the production of higher trophic levels (such as fish) declines with nutrient loading and system productivity, but the underlying mechanisms are poorly understood. Here we show that in seston (fine particles in water) during summer, specific omega3-polyunsaturated fatty acids (omega3-PUFAs), which are important for zooplankton, are significantly correlated to the trophic status of the lake. The omega3-PUFAs octadecatetraenoic acid, eicosapentaenoic acid (EPA) and docosahexaenoic acid, but not alpha-linolenic acid, decrease on a double-logarithmic scale with increasing total phosphorus. By combining the empirical relationship between EPA-to-carbon content and total phosphorus with functional models relating EPA-to-carbon content to the growth and egg production of daphnids, we predict secondary production for this key consumer. Thus, the decreasing efficiency in energy transfer with increasing lake productivity can be explained by differences in omega3-PUFA-associated food quality at the plant-animal interface.     

Symbiotic fungal endophytes control insect host-parasite interaction webs

Symbiotic microorganisms that live intimately associated with terrestrial plants affect both the quantity and quality of resources, and thus the energy supply to consumer populations at higher levels in the food chain. Empirical evidence on resource limitation of food webs points to primary productivity as a major determinant of consumer abundance and trophic structure. Prey quality plays a critical role in community regulation. Plants infected by endophytic fungi are known to be chemically protected against herbivore consumption. However, the influence of this microbe-plant association on multi-trophic interactions remains largely unexplored. Here we present the effects of fungal endophytes on insect food webs that reflect limited energy transfer to consumers as a result of low plant quality, rather than low productivity. Herbivore-parasite webs on endophyte-free grasses show enhanced insect abundance at alternate trophic levels, higher rates of parasitism, and increased dominance by a few trophic links. These results mirror predicted effects of increased productivity on food-web dynamics. Thus 'hidden' microbial symbionts can have community-wide impacts on the pattern and strength of resource-consumer interactions.     

Sedimentation rates and provenance analysis in the Southwestern Okinawa Trough since the mid-Holocene

As a high-sedimentation rate depocenter along the path of the Kuroshio Current, the southwesternmost part of the Okinawa Trough is a key area to understand the Kuroshio history and sediments transpor-tation. A 34.17-m-long sediment core was obtained by the advanced piston corer of Marco Polo/IMAGES XII MARION DUFRESNE during the May 2005 from the Southern Okinawa Trough at site MD05-2908. The recovered sediments were analyzed by AMS ¹⁴C dating, coarse size fraction (>63 μm) extraction and moisture content determination in order to study its sedimentation flux and provenance. The depth-age relationship of core MD05-2908 was well constrained by 17 ¹⁴C dating points. The sediments span across the mid-Holocene (6.8 ka B.P.) and have remarkablely high sedimentation rates between 1.8 and 21.2 m ka^-1, which is well consistent with the modern observations from sediment traps. We identified five 70―200 a periods of abnormally rapid sedimentation events at 6790―6600 a B.P., 5690―5600 a B.P., 4820―4720 a B.P., 1090―880 a B.P., and 260―190 a B.P., during which the highest sedimentation rate is up to 21.2 m/ka. In general, the lithology of the sediments were dominated by silt and clay, associated with less than 5% coarse size fraction (>63 μm). As the most significant sediment source, the Lanyang River in northeastern Taiwan annually deliver about 10Mt materials to the coastal and offshore region of northeast Taiwan, a portion of which could be carried northward by currents toward the study area. Therefore, we concluded that the 5 abnormally rapid sedimentation events may be related to intensified rainfall in Taiwan and thus increased materials to our study area at that time. However, a few ex-treme-rapid sedimentation events cannot be explained by normal river runoff alone. The large earth-quakes or typhoons induced hyperpycnal discharge of fluvial sediment to the ocean may also act as a potential source supply to the Okinawa Trough.     

Pulleniatina Minimum Event during the last deglaciation in the southern South China Sea

The planktonic foraminiferal faunal census of core MD 05-2894 （7°2.25′N, 111°33.11′E, water depth 1982 m）, retrieved from the southern South China Sea （SCS） during the ＂Marco Polo＂ cruise in 2005, was performed to investigate the abundance changes of a subsurface dweller, Pulleniatina obliquiloculata. The results display that the abundance of P. obliquiloculata nearly declines to zero during 16.0--14.9 ka, corresponding to the Heinrich 1 （H1） cold interval. The unexpected decrease of P. obliquiloculata occurs in the adjacent cores, roughly between 17 and 14.8 ka based on the previous studies. Accordingly, the Pulleniatina Minimum Event in the last deglaciation can serve as a good stratigraphical indicator, at least in the southern SCS. To further explore the changes of sea surface temperature （SST） and subsurface seawater temperature （SSST）, we made parallel Mg/Ca measurements on surface dweller Globigerinoides tuber and subsurface dweller P. obliquiloculata tests. Since the last deglaciation, the SSTs show a continuous increasing trend towards the late Holocene, while the warming of the subsurface water is punctuated by a 2℃-cooling interval across the deglacial Pulleniatina Minimum Event. Both increased 5180 differences between G. ruber and P. obliquiloculata, and increased temperature differences between surface and subsurface water suggest a shoaling of the mixed layer during the deglacial Pulleniatina Minimum Event. Therefore, we consider that the significant changes in the upper ocean structure are responsible for the Pulleniatina Minimum Event during the last deglaciation in the southern SCS.