舟山带鱼线粒体COI基因SNP位点分析

为探讨舟山带鱼种质状况,本研究利用直接测序法对舟山带鱼线粒体COI基因进行SNP挖掘分析,获得长度一致的985 bp基因片段,检测到22个SNP位点,对SNP位点在样品中分布情况分析,发现部分SNP位点分布情况类似甚至完全相同,推测基于线粒体COI基因片段不同位点突变可能存在关联性。     

载脂蛋白E和SHCP66的基因单核苷酸多态性及其与长寿性状的相关研究

研究载脂蛋白E（Apolipoprotein E,APOE)和SHCP66（Src homology-domain cotaining protein,66ku)的基因在一个中国隔离人群中的单核苷酸多态性（single nucleotide polymorphism,SNP)，以及它们与长寿性状的相关性，应用多聚酶链式反应结合测序技术对2个基因的外显子区域进行小样本（40个随机个体）的单碱基多态位点（SNP）检测，并对其中apoe的2个侯选位点在大样本中进行基因分型，结果在apoe基因中发现3个SNP位点，其中一个在调控区内的位点是首次报道，shcp66发现3个SNP位点和一个缺失突变，均为首次报告.apoe的2个候选位点在隔离人群中的长寿个体及对照组中基因频率的卡方测验显示无显著差别（P＞5％），因此，在该长寿人群中，该基因的多态现象与长寿性状无显著的关联。     

郑州地区线粒体基因突变与2型糖尿病相关性分析

探讨线粒体t RNALeu(UUR)基因3243位点和NADH脱氢酶亚单位1(ND1)3394位点突变在河南省郑州地区2型糖尿病(type 2 diabetes mellitus,T2DM)人群中的发生率及其临床特征。随机抽取无血缘关系的T2DM患者295例及正常对照250名,采用聚合酶链反应—限制性片段长度多态性(PCR-RFLP)技术进行线粒体t RNALeu(UUR)基因3243位点及3394位点突变检测,比较线粒体基因突变在两组间分布的差异,并对样本的相关生化指标进行测定,从而得出河南省郑州地区2型糖尿病与线粒体基因点突变的相关性。2型糖尿病组中mt DNA 3243突变率为0.68%,mt DNA 3394突变率1.36%,在正常对照组未检出mt DNA 3243位点突变,mt DNA 3394突变率0.80%,组间基因型差异用χ2检验,各位点突变率差异均无统计学意义(p0.05)。胰岛素水平和线粒体呼吸链酶复合物Ⅰ活性指标在有无突变组之间有明显差别(p0.05)。线粒体t RNALeu(UUR)基因3243和ND1区3394位点突变不是河南省郑州地区2型糖尿病的主要病因,仅为人群中线粒体DNA的基因多态性。但此位点突变能引起空腹胰岛素水平和线粒体呼吸链酶复合物Ⅰ活性的下降,推测其对2型糖尿病的发生的其他因素有协同作用。     

藏族和夏尔巴人15个常染色体STRs的遗传多态性

为了探究生活在西藏自治区的藏族人和夏尔巴人的亲缘关系,选取西藏日喀则地区的藏族人225例和夏尔巴人181例,对他们的15个常染色体遗传标记短串联重复序列(short tandem repeat,STRs)进行基因分型,将二者与一些高原人群及世界其他人群进行比对.采用Cervus3.0、Arlequin3.5、Structure2.3.4、R3.0.3、Distruct1.1等统计分析软件,对高原人群的遗传多态性进行分析.在藏族人群中共检测出141个等位基因,频率分布在0.002 2～0.622 2之间;在夏尔巴人群中共检测出139个等位基因,频率分布在0.002 8～0.569 1之间. 15个位点中,藏族人群的TPOX、D3S1358、TH01位点和夏尔巴人群的D18S51、FGA位点的多态性不高,其余位点均属于高度多态性遗传标记.在STRs遗传结构上,藏族人和夏尔巴人与东亚人群最为接近,与非洲人和欧洲白人相似程度较低.由此认为,藏族人和夏尔巴人都属于东亚人群的一员,高原人群与东亚人群有着共同的遗传结构.     

新疆锡伯族与哈萨克族间3个STR位点的多态性比较

分别获得CSF1PO,TPOX和TH013个短串联重复序列STR,对新疆锡伯族和哈萨克族人群中等位基因频率、基因型频率及相关法医学数据进行比较。应用PCR技术、4%变性聚丙烯酰胺凝胶电泳及银染技术对上述3个STR位点分型。锡伯族人群CSF1PO位点有9个等位片段,TPOX位点有8个等位片段,TH01位点有8个等位片段;哈萨克族人群CSF1PO位点有8个等位片段,TPOX位点有8个等位片段,TH01位点有7个等位片段,两民族各自的3个STR位点的基因型分布均符合Hardy-Weinberg平衡;锡伯族人群各位点杂合度分别为0.9426,0.8361,0.8853,多态信息量分别为0.8298,0.7213,0.7626;哈萨克族人群各位点杂合度分别为0.8753,0.8777,0.9321,多态信息量分别为0.7401,0.7568,0.7509。以上3个STR位点的基因频率分布在两个不同的人群中具有显著的差异。     

广东汉族人群LDL受体基因PvuⅡ多态性位点的研究

建立了ＬＤＬ受体基因内含子１５ｐｖｕⅡ多态性位点的ＰＣＲ－ＲＦＬＰ技术．利用该技术从人类外周血基因组ＤＮＡ中分离到一个长为０．８１ｋｂ的片段,核酸序列测定证明分离的片段是ＬＤＬ受体基因内含子１５．对广东汉族人群内含子１５的ＰｖｕⅡ位点多态性状况进行了研究,实验显示：广东汉族人群ＬＤＬ受体基因中存在着ＰｖｕⅡ多态性位点;２１２个ＬＤＬ受体等位基因ＰｖｕⅡ酶切位点出现的频率为０．１６,说明ＰｖｕⅡ多态性位点可以作为广东人群的遗传标记．     

内蒙古和林格尔东周时期古代人群的分子遗传学分析

对10个古代个体的线粒体DNA高可变Ⅰ区进行了扩增和测序. 基于和林格尔古代人群与现今相关欧亚人群的mtDNA序列， 进行了系统发育分析和多维尺度分析. 研究结果表明, 和林格尔古代人群在母系遗传上与现在北亚人群的亲缘关系最近. 结合考古学、 人类学以及分子生物学的研究， 推断这个古代人群是从蒙古高原以及外贝加尔地区南下迁移至今天的内蒙古和林格尔地区的游牧人.     

中国汉族人群线粒体DNA单倍群与尿酸水平的关联研究

尿酸(UA)是人体内嘌呤核苷酸代谢的最终产物，与多种慢性疾病相关。因线粒体处于人体代谢的核心位置，线粒体DNA变异可能影响血液中尿酸水平。本研究对来自广西南宁、江苏泰州和河南郑州的3个中国汉族人群共计2 837例样本(其中男性样本1 112例、女性样本1 725例)进行分析，对样本初始的UA值矫正了性别和年龄因素。本研究根据线粒体DNA的变异将受试者分为16种基础单倍群，通过比较每个单倍群样本和其他样本的UA水平差异，探索线粒体单倍群和UA的相关性。在总体3个汉族人群中，M8单倍群的UA矫正值((310.5±87.2)μmol/L)显著低于其他单倍群((326.1±80.6)μmol/L,P=0.02),B5单倍群的UA矫正值((338.8±72.5)μmol/L)显著高于其他单倍群((324.9±81.2)μmol/L,P=0.01)。本研究结合后续分析发现，在总人群、南宁人群和泰州人群女性数据中，M8单倍群均与低UA水平显著相关；在总人群和女性数据中，B5单倍群与高UA水平显著相关。综上所述，本研究在中国汉族人群中发现了与UA水平显著相关的线粒体单倍群，为后续线粒体DNA变异在尿酸...     

ATP基因突变与乳腺癌相关性分析

对41例女性乳腺癌患者带毛囊毛发线粒体DNA的编码区ATP基因(ATP6和ATP8)进行测序,对比45例正常人群线粒体DNA基因序列,采用统计学分析软件筛选具有统计学差异的突变位点,共检测到ATP基因突变位点25个,其中在ATP6亚基上共发现18个(72％)基因突变,其中8584G-A、8701A-G、8794C-T、...     

四川省结核分枝杆菌基因分型中MIRU-VNTR位点稳定性评价

摘要: 本文收集四川省2009-2013年184株结核分枝杆菌菌株，用标准12 MIRU-VNTR位点组对四川省结核分枝杆菌菌株进行基因分型研究，评估四川地区一线VNTR分型位点的稳定性，确定四川地区保守位点组合及主要MIT型。184株结核分枝杆菌基因分型后得到51个基因型，12个基因型成簇。本研究确定当前四川地区12高分辨力位点VNTR分型方法中的7个MIRU位点依然具有较高的分辩能力（h > 0.55）。MIRU02, MIRU20, MIRU23, MIRU24为多态性最低的4个位点（0 ≤ h < 0.4），保守位点组的主要MIT型为“2251”。四川省结核分枝杆菌VNTR位点的多态性保持稳定，目前采用的12高分辨力VNTR位点依然稳定可靠，本研究首次确定了四川地区保守位点组及主要MIT型，为下一步结核分枝杆菌进化研究奠定了基础。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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