一株产碱性蛋白酶菌株的分离与鉴定

从黄石市土壤中分离到一株产碱性蛋白酶的革兰氏阴性杆菌,不产芽抱,具美膜．能在ｐＨ９－１１条件下生长、产酶．产酶最适ｐＨ值为９,最适温度为３７℃,适发酵时间为３６ｈ．发酵产酶量为４５．０Ｕ·ｍＬ－１根据实验结果,该菌株鉴定为毛状假单抱菌Ｐｓｅｕｄｏｍｏｎａｓ　ｌａｓｉａ．     

产耐热蛋白酶菌株的分离和酶性质的研究

从南汇县黄路乡一草堆下的土壤中分离到一株产耐热蛋白酶的链霉菌。该酶的最适反应温度为７０℃。在６０℃时稳定，当８０℃时处理１０ｍｉｎ，尚余８４％活性。在Ｃａ＾２＋存在的条件下，酶的热稳定性有显著提高。酶反应的最适ｐＨ为８．５，在ｐＨ５～９之间稳定，因此属于微碱性蛋白酶。通过抑制剂试验，该酶基本上被ＰＭＳＦ（苯甲基磺酰化合物）抑制，但不被金属螯合剂（ＥＤＴＡ，双吡啶）抑制。金属离子Ｃｕ＾２＋，Ｚｎ＾２     

产黄纤维单胞菌纤维素酶的培养条件

对产黄纤维单胞菌所产纤维素酶的条件和天然纤维素能力的利用进行了研究,认为该菌产酶最适起始ＰＨ为６．０最适产酶温度为２５℃,８－１６ｈ为产酶高峰期;ＣＭＣＮａ,酪蛋白是该菌最好的碳氮源,高浓度的葡萄糖抑制纤维素酶的形成,而纤维二糖是该菌的较好碳源和诱导剂。     

分解纤维素菌HT3的筛选及酶活力测定

从不同榈中分离出能分解纤维素菌２９株，经复筛得到产纤维素酶活力较高菌有６株，其中ＨＴ３细菌酶活力最高。该菌产酶为胞外分泌，在最适条件下培养，酶活力可达５．１Ｕ。     

白腐菌多酚氧化酶研究

研究了彩绒革盖菌、多孔菌和毛栓菌多酚氧化酶的产酶曲线，及酶作用的最适pH值、最适酶解温度对酶活的影响．结果表明，来自不同白腐菌的多酚氧化酶其产酶曲线不同，酶的特性也有差别．     

磁性载体用于酶固定化方面的研究

合成了具有磁响应性的两亲性聚乙二醇胶体粒子,并以此为载体,用吸附交联法固定化了α－淀粉酶。最适交联条件研究表明：缓冲液ｐＨ值、戊二醛浓度及加酶量都对固定化酶活力、比活有一定影响。在最适固定化条件下,固定化酶的活力为３４０００Ｕ·ｇ＾－１干胶,蛋白载量为１００ｍｇ·ｇ＾－１干胶,比活为３４０Ｕ·ｍｇ＾－１蛋白,活性回收率为３７．７％。最适反应温度比天然酶（５０℃）提高３０℃。最适ｐＨ比天然酶（７．０     

紫外诱变原生质体选育碱性蛋白酶高产菌株的研究Ⅲ.诱变株的选育及其产酶条件的研究

以地衣芽孢杆菌（ＢａｃｉｌｕｓＬｉｃｈｅｎｉｆｏｒｍｉｓ）５３号菌为出发菌株，在原生质形成和再生的最佳条件下制备原生质体，并对原生质体进行了多次诱变处理，从大量突变株进行筛选最终获得了高产、稳定、耐热的碱性蛋白酶产生菌株５３－Ｇ３８－６，产酶活力由１１０４Ｕ／ｍｌ提高到２２０８０Ｕ／ｍｌ．该诱变株最适产酶条件为：培养基由质量分数（ｗ／％）为胰蛋白胨１，酵母膏０．５，玉米粉５，Ｎａ２ＨＰＯ４１２Ｈ２Ｏ０．４，ＫＨ２ＰＯ４０．０３，Ｎａ２ＣＯ３０．１组成，ｐＨ自然，培养温度４２℃，培养时间４４～４８ｈ，ｗ／％为０．２的ＣａＣＯ３可提高酶的产量．酶作用的最适条件：６２℃，ｐＨ１０．０，ｐＨ在９～１０之间稳定     

胃蛋白酶催化壳聚糖降解的特性研究

采用粘度测定法和还原糖测定法,研究了温度、ＰＨ值、反应时间、底物浓度、金属离子及壳聚及壳聚糖的脱乙酰化度对胃蛋白酶降解活性的影响,确定了以壳 底物的猪胃酶的一些催化特性：最适温度为７０℃,最适ＰＨ值为５．０,米氏常数Ｋｍ为８．９４＊１０＾－３ｇ．ｍＬ＾－１,一定浓度的Ｃａ＾２＋,Ｃｕ＾２＋,Ｂａ＾２＋,Ｍｎ＾２＋抑制该酶的活性。结果表明,随着壳聚糖脱乙酰化度的提高,胃蛋白酶活性降低。     

酶法制备毛蚶汁调味品的研究

本文研究了以毛蚶肉为原料，酶法制备毛蚶汁调味品的方法。酶解条件如下：最适酶解温度４０－４５℃，最适加中性蛋白酶ＡＳ１．３９８，加量为１．０％（湿基），酶解时间为２０－２４小时。酶解后，产品在自然温度（１５一２０℃）下酿造２个月。再对产品进行调色、调香、增稠。成品符合ＳＢ１０７－８３鱼露一级标准。     

嗜热蛋白酶生产菌的筛选

从温泉水、高温堆肥等样品中初筛到62株嗜热蛋白酶生产菌,进一步复筛出一株高产嗜热蛋白酶的生产菌株DPE7(菌株来自温泉水),该菌株被初步鉴定为芽孢杆菌(Bacillus sp．)．嗜热蛋白酶生产的最适碳源是葡萄糖, 最佳氮源为酵母粉加硫酸铵,Ca2 对菌株产酶具有促进作用．在65℃、175 r／min、pH 9的产酶培养基中振荡培养 24 h后,发酵液中的酶产量高达23．4 U／mL(60℃测定)．     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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