罗丹明B荧光光谱机理的研究

考察了罗丹明B浓度、溶剂的极性、pH值大小、表面活性剂的增敏等因素对罗丹明B的荧光光谱影响，探讨了以上各因素影响罗丹明B的荧光光谱的机理．结果表明，随着罗丹明B浓度的增加，荧光光谱波长红移，强度先增后减；溶剂极性增加，波长红移，荧光强度则由于溶剂特殊效应的影响而减小；pH=1．0～3．0时，荧光强度随pH的增加而增大，pH＞3．0时，荧光强度几乎无变化；阳离子表面活性剂对体系的荧光强度具有增敏作用，阴离子表面活性剂则相反．     

基于苯并噻二唑衍生物的荧光传感器检测铜离子和氟离子（英文）

报道了一种基于苯并噻二唑衍生物的荧光传感器用于检测金属铜离子和氟离子。通过紫外可见光谱和荧光光谱研究此传感器检测离子的能力。研究表明,在所测试的阴离子中,只有氟离子可以引起此荧光探针的吸收峰蓝移(从390 nm到383 nm),并伴随着一定强度的减弱。同样氟离子可以引起此荧光探针的荧光强度降低并且蓝移(从496 nm到488 nm)。在所测试的阳离子中,铜离子可引起荧光探针的吸收峰红移,荧光强度淬灭90%。此外,当加入铜离子和氟离子的先后顺序不同时,引起的荧光光谱变化也不同。     

几种芴基衍生物荧光性质的初步研究

以芴为原料合成了芴酮,芴醇,2,7-二溴基芴,2,7-二溴基芴酮,2,7-二溴基芴醇,二茂铁甲酸芴酯等芴基衍生物.测定了所合成芴基衍生物的荧光光谱,并讨论了结构对荧光光谱的影响.结果表明,共轭结构对荧光光谱的峰形影响很大;溴代略微影响光谱峰形,同时使特征峰轻微红移,强度略有下降,而不同发射波长峰的强度则发生无规律性变化,表明其他基团(如羟基)与溴代基团对化合物荧光光谱产生协同效应.     

猪心肌高铁肌红蛋白荧光光谱测定条件的确定及功能域的指认

以纯猪心肌高铁肌红蛋白(p Met Mb)为对象,建立荧光光谱条件并经同步荧光指认其主要功能域.研究表明,以600 nm/min扫描速度和中等响应时间为基本条件,p Met Mb的适宜荧光光谱条件分别是20 mmol/L p Met Mb、10 nm狭缝宽度和270 nm或430 nm为激发光波长.经同步荧光光谱解析,酪氨酸(Tyr-)、色氨酸(Trp-)和铁卟啉环(heme-Fe3+)荧光特征峰分别在312、355和630 nm处.270 nm是Trp-和Tyr-适宜激发光波长,而430 nm是heme-Fe3+适宜激发光波长.与马肌红蛋白(h Mb)比较,其Trp-和heme-Fe3+荧光光谱特征峰出现红移.430 nm可激发630 nm处heme-Fe3+,发生电子迁移和能量跃迁.随着Trp-和heme-Fe3+特征峰的红移,推测p Met Mb中heme-Fe由五配位高自旋(Fe2+)转变为六配位低自旋(Fe3+).     

芘有机硼化合物的合成及对氟离子的识别

通过在硼原子上引入大体积的芳香取代基合成稳定的含芘二米基硼化合物(Ⅰ)。经紫外可见吸收光谱和荧光光谱分析,研究其光物理性质及对F-的识别性质。结果表明:化合物具有较强的蓝色荧光发射,在四氢呋喃(THF)中荧光峰位为428 nm;由弱极性溶剂正己烷(荧光峰位在413 nm处)到强极性溶剂乙腈(荧光峰位在435 nm处),化合物荧光光谱峰值红移22 nm,荧光量子产率由0.98降为0.68;将四丁基氟化铵逐渐滴加入化合物Ⅰ的THF溶液中,其吸收光谱和荧光光谱均伴有旧峰消失和新峰出现的现象:吸收光谱的旧峰在383和399 nm处,新峰出现在324、338和353 nm处;荧光光谱的旧峰在429 nm处,新峰出现在385和403 nm处;继续滴加四丁基氰化铵,其吸收光谱和荧光光谱均无明显变化。     

十二烷基硫酸钠对猕猴桃蛋白酶的变性作用

应用光谱法观测酶的结构变化,紫外差光谱显示在233nm有一较明显的差吸收负峰,在286nm、300nm左右有不大的负峰和在265nm的正峰;荧光谱显示327nm的相对荧光强度随SDS浓度的增高而递减;发射峰位置红移,酶受SDS>4.1mmol/1作用1h,可引起310nm新荧光发射肩。动力学资料表明,SDS对酶的变性过程为一级反应;酶的失活分快慢相,呈现二个一级反应,同时测定在SDS作用下actinidin的变性与失活速度,表明酶的失活速度大于变性速度,似可认为低浓度SDS能使结构域相错开,从而敏感地改变活性部位的微区结构,导致酶快速失活,提高SDS浓度则使酶的结构域发生构象变化,引起光谱法所显示的特征。     

肌红蛋白的活性中心铁卟啉与金属离子相互作用的研究（I）——与铜离子的相互作用

用荧光光谱和UV-Vis吸收光谱技术研究了肌红蛋白(Mb)和Cu^2 的相互作用，结果表明在水溶液中Cu^2 的加入使Mb中血红素在597nm处的荧光峰强度降低，也使Mb在407nm处的Soret带的吸收峰强度减弱，清楚地表明Cu^2 与Mb发生了相互作用。     

用蛋白内源荧光考察溶液pH对PSⅡ两种外周蛋白构象的影响

借助278nm和295nm激发的内源荧光光谱分析，考察不同pH值缓冲液中23kD、17kD蛋白构象变化．结果表明：295nm波长光激发时，23kD蛋白具有326nm荧光发射峰和360nm副峰；17kD蛋白具有328nm荧光发射峰和355nm副峰；278nm波长光激发时，23kD和17kD蛋白的最大荧光发射峰均在306nm处．与中性条件下相比，酸性(pH3．0～4．0)或碱性(pH8．0～9．0)缓冲液中，23kD蛋白和17kD蛋白的内源荧光光谱都发生显著变化：最大荧光峰位置和强度均不相同，340nm-360nm荧光发射副峰的强度增加．反映溶液中两种蛋白的构象易发生改变，离子键和氢键是维系23kD、17kD蛋白构象的重要因素之一。     

卟啉-脂质体化合物P-GlyL的紫外可见及荧光光谱性质研究

以溴代肽脂质BrC5Gly2C16与5,10,15-三苯基-20-对羟基苯基卟啉（HPTPP）为原料，合成一种可用作新型分子器件的卟啉-脂质体化合物P-GlyL。 采用元素分析、紫外可见光谱、红外光谱以及核磁共振等进行表征。 并对该化合物在不同浓度的金属离子Zn2+溶液中的紫外可见光谱和荧光光谱进行了研究。 结果表明，当加入Zn2+之后，形成的金属卟啉化合物荧光强度在650.0nm处下降，而在437.0nm和595.8nm处产生新的荧光发射峰，且荧光强度随着Zn2+浓度增加而增强，而同时349.0nm和698.5nm处的发射峰发生了0.5nm的红移且荧光强度增强。     

肌红蛋白的活性中心铁卟啉与金属离子相互作用的研究(Ⅰ)--与铜离子的相互作用

用荧光光谱和UV Vis吸收光谱技术研究了肌红蛋白(Mb)和Cu2 的相互作用,结果表明在水溶液中Cu2 的加入使Mb中血红素在597nm处的荧光峰强度降低,也使Mb在407nm处的Soret带的吸收峰强度减弱,清楚地表明Cu2 与Mb发生了相互作用。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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