形态变换序列与分析

本文在基本形态运算的基础上，提出交替顺序滤波ＡＳＦ，分水岭ｗａｔｅｒｓｈｅｄ变换形态分割等新方法，同时讨论了形态谱分解与重构，形态分形等新的领域，文中所述的各种形态变换序列在图象分析中有广阔的应用前景。     

关于最大长度序列的稳定性

讨论了最大长度二元序列Ｓ＾∞的稳定性 ，给出集合Ａ，使得ＷＣｋ（Ｓ＾∞ＫＬ（Ｓ＾∞）当且仅当Ｋ∈Ａ；由此证明了：当Ｌ（Ｓ＾∞）是素数时，Ｓ＾∞的线性复杂度稳定性比较理想。     

非线性代数方程组的正解数

利用关于一元多项式实根个数的显式判准、多项式的判别式序列、判别矩阵的顺序主子式序列和Sturm序列的变号数，给出了关于二元非线性代数方程组正解数的一个显式判准(假设方程组的解是有限的)．     

混合顺序作业堆场BAY优化模型

介绍了集装箱堆场优化的重要意义,提出了混合顺序堆场作业的概念,在图搜索技术和模式识别理论的基础上建立了随机条件下的混合顺序作业堆场ＢＡＹ优化模型,计算机模拟结果证明：混合顺序作业堆场ＢＡＹ优化模型可以在堆场作业不倒箱的情况下,产生符合配载要求的堆场。     

关于Beatty序列的加法

设［ｘ］表示ｘ的整数部分,Ａｘ（α）＝｛［αｎ］：ｎ∈Ｎ,αｎ≤ｘ｝,Ａｘ（β）＝｛［βｎ］：ｎ∈Ｎ,βｎ≤ｘ｝．作者给出了｜Ａｘ（α）＋Ａｙ（β）｜的估计式．     

细茎大豆（G.gracilis）rbcS基因结构与分子进化分析

从细茎大豆的嫩叶中提取总ＤＮＡ，用ＰＣＲ方法扩增得到包含完整编码区的ｒｂｃＳ基因并将其克隆到ｐＢＬＵＥＳＣＲＩＰＴ载体中。完成全基因１０８９个核苷酸测序后，运用ＰＣＧＥＮＥ进行顺序编辑和同源比较，并应用ＭＥＧＡ１．０２１软件中的Ｎｅｉｇｈｂｏｒ－ｊｏｉｎｉｎｇ方面画出Ｒｕｂｉｓｃｏ小亚基仓的系统进化树。     

黄淮海平原历史旱涝灾害的时间序列分析

选用全国自然灾害历史数据库中黄淮海平原地区的旱涝灾害等级数据，运用时间序列分析方法，建立了该地区旱涝灾害的自回归－－移动平均混合模型（ＡＲＭＡ模型），并根据所建模型对旱涝灾害进行了后验预报，结果表明，用ＡＲＭＡ模型做年际旱涝变化趋势预报是有潜力的。     

时间序列AR模型参数的积分求解法

基于对时间序列实质的分析，提出了旨在减少序列的随机误差影响以及提高拟合精度的ＡＲ模型参数的积分求解法，重点讨论了ＡＲ（１）模型及ＡＲ（ｐ）模型参数的积分求解法，并与最小二乘法在计算机上进行了仿真比较，结果表明，有用积分求解法所得ＡＲ模型参数的估计精度比最小二乘法的高。     

ARMA序列及其功率谱估计若干新的理论和方法

在介绍了ＡＲＭＡ序列与功率的基础上升，论述了ＡＲ模型定阶与参数估计的ＳＶＤ－ＴＬＣ算法，以及ＭＡ模型定价与参数计算和ＡＲＭＡ过程自相关序列的计算。     

GPS定位数据的在线识别与预测

通过大量的ＧＰＳ定位观测数据的统计处理导出了一种对非平稳时序列建模或识别及预测的简单方法。该方法是 基于小二乘法和线性系统识别方法。并提出了与传统ＡＲＭＡ模型有所不同的模型，我们称之为准ＡＲＭＡ模型，用具时变参数的模型对ＧＰＳ接收机输出数据进行线识别与预测，证实了此方法有效方法。     

三种ARS片段对水稻部分酶解小细胞团转化频率的影响

ＡＲＳ２,ＡＲＳ７,ＲＳ８是从水稻珍汕９７Ａ不育系统ＤＮＡ中的克隆的一组在酵母细胞中能直始ＤＮＡ复制的片段。其中ＡＲＳ８是单考贝而ＡＲＳ２,ＡＲＳ７却是高度重复顺序。将带有ＡＲＳ２,ＡＲＳ７,ＡＲＳ８的质粒用低压脉冲电泳法导入水稻部分酶解小细胞团中,经浅层培养,铺板,获得愈伤组织。     

一种简便有效的PCR扩增片段直接回收克隆方法

作者提供一种简便快速的克隆方法，即直接对琼脂糖凝胶电泳分离的PCR产物进行克隆，可获得较高的阳性克隆率.结果显示，300～700 bp大小的片段阳性克隆率为70.72%，300 bp以下和大于700 bp的片段阳性克隆率分别达到60.38%和45.33%.该方法适用于含有大量不同大小DNA片段的PCR产物并需要同时对其进行回收和克隆的样品，不仅可以大大简化实验过程，也可以降低假阳性出现的机会.     

Nucleosome positioning can affect the function of a cis-acting DNA element in vivo

Positioning of nucleosomes has been proposed as one mechanism whereby the activity of DNA is regulated: cis-acting elements located in linker DNA might be more accessible for interaction with trans-acting protein factors than they would be if they were directly associated with histones in nucleosome core particles. The eleven base pairs constituting the autonomously replicating sequence (ARS) of the high-copy-number TRP1ARS1 plasmid of Saccharomyces cerevisiae are located in a linker region near the edge of a positioned nucleosome and form an origin of replication. Could nucleosome positioning render the ARS accessible for interaction with the proteins necessary for its function? I have tested this hypothesis by making deletions and an insertion to move the ARS into the nucleosome DNA and then examining the effects on ARS function. There is a marked decrease in copy number when the ARS is moved into the central DNA region of the nucleosome core particle, a region known to differ in structure and stability from the peripheral segments of nucleosome DNA.     

逆转录聚合酶链式反应快速检测水稻条叶枯病毒

逆转录聚合酶链式反应（Ｒｅｖｅｒｓｅ Ｔｒａｓｃｒｉｐｔｉｏｎ ａｎｄ Ｐｏｌｙｍｅｒａｓｅ Ｃｈａｉｎ Ｒｅａｃｔｉｏｎ,ＲＴ－ＰＣＲ）具有灵敏度高,准确性好的特点,因而被广泛应用于植物病毒检测。在ＲＴ反应中,ＲＮＡ样品的快速制备尤为重要用异丙醇二步沉淀法获得ＲＮＡ,快速检测水地片中ＲＳｔＶ伯ＲＴ－ＰＣＲ方法,可使测定时间缩短至６ｈ,并可从０．０７８ｍｇ感病叶片中检测出ＲＳｔＶ。     

水稻苯达松敏感致死基因的RAPD标记的克隆及测序

用3S Spin DNA Agarose Gel Purification Kit试剂盒将与水稻苯达松敏感致死基因相连锁的RAPD遗传标记S20—420和S316—600回收纯化，连接于pGEM—T载体并克隆测序，得到了S20—420和S316—600的全序列，其长度分别为423bp、606bp．将两端序列设计特异PCR扩增引物可用于检测水稻苯达松敏感致死基因和标记辅助育种．     

Microdissection of chromosome 7B of common wheat and cloning of low-copy specific DNA sequences

The 7B chromosome of common wheat was microdissected from pollen mother cells of the 7B monosomic line of common wheat cv. Chinese Spring (CS). After proteinase K and DNA topoisomerase Ⅰ treatments, the isolated chromosomes were subjected to 1-3 rounds of DOP-PCR amplification, which produced continuous DNA fragments ranging from 150 to 700 bp. Ge-nomic Southern hybridization confirmed that the PCR products were originated from the wheat genome. Cloning of portion ( > 200 bp) of the 3rd round DOP-PCR products (50 μL) could generate about 20 000 recombinant clones. Characterization of 50 randomly chosen clones indicated that 21 clones produced discrete PCR products with the size of 240-600 bp. Dot-blot hybridization showed that among the 21 clones, 11 ( ～ 55%) were of low-copy nature while 10 ( ～ 45%) were repetitive. Southern hybridization with the complete set of the CS "nullisomic-tetrasomic (NT)" lines demonstrated that all the 6 low-copy clones were specific to either chromosome 7B or the 7th homoeologous group, whereas the 3 arbitrarily chosen repetitive clones were non-specific, disperse sequences.     

油茶基因组微卫星特征分析

对油茶基因组约10%覆盖度的DNA序列进行微卫星查找,共获得11 344个重复单元长度为1～6碱基的微卫星。在此基础上,通过对这些微卫星序列分析发现:在油茶基因组中长度为二核苷酸的微卫星重复单元最为丰富,占27.1%;在单碱基重复和二碱基重复这两种类型中,最主要的优势重复单元分别是A/T以及AT/TA、AG/TC。三碱基、四碱基、五碱基重复类型中,(AAN)n、(AAAN)n和(AAAAN)n为对应的优势重复单元,这些优势重复单元中富含碱基A和T。油茶基因组中变异程度高的微卫星(长度≥20 bp)约占11.7%。分析还发现,除单核苷酸重复微卫星外,油茶基因组微卫星长度的变异速率与重复单元长度呈负相关,即油茶基因组中长度较短的微卫星变异速率较快,而较长的重复单元变异速度较慢,相对较为稳定。     

Antisense expression of a rice cellular apoptosis susceptibility gene (OsCAS} alters the height of transgenic rice

Cellular apoptosis susceptibility (CAS) gene plays important roles in mitosis, development and export of importin a from the nucleus, but its function in plant is unknown. In this study, a rice CAS ortholog (OsCAS), which encodes a predicted protein of 983 amino acids with 62% similarity to human CAS, was identified. DNA gel blot analysis revealed a single copy of OsCAS in the rice genome. A 973 bp fragment at the 3' end of OsCAS cDNA was cloned from rice cDNA library and transferred into rice in the antisense direction under the control of CaMV 35S promoter via Agrobacterium-mediated transformation method, 105 transgenic lines were obtained. Expression of OsCAS was suppressed in the antisense transgenic lines as revealed by semi-quantitative RT-PCR. The antisense transgenic lines showed dwarf phenotypes. The results indicated that OsCAS was involved in culm development of rice.     

Antisense expression of a rice cellular apoptosis susceptibility gene (OsCAS) alters the height of transgenic rice

Cellular apoptosis susceptibility (CAS) gene plays important roles in mitosis, development and export of importin α from the nucleus, but its function in plant is unknown. In this study, a rice CAS ortholog (OsCAS), which encodes a predicted protein of 983 amino acids with 62% similarity to human CAS, was identified. DNA gel blot analysis revealed a single copy of OsCAS in the rice genome. A 973 bp fragment at the 3′ end of OsCAS cDNA was cloned from rice cDNA library and transferred into rice in the antisense direction under the control of CaMV 35S promoter via Agrobacterium-mediated transformation method, 105 transgenic lines were obtained. Expression of OsCAS was suppressed in the antisense transgenic lines as revealed by semi-quantitative RT-PCR. The antisense transgenic lines showed dwarf phenotypes. The results indicated that OsCAS was involved in culm development of rice.