A population of human lymphocytes staining for esterases.

A population of lymphocytes is found to stain positively for esterases. The positively staining lymphocytes are more predominant among T than B lymphocytes and are significantly increased on stimulation with PHA. Treatment with cholinestrase inhibitors reduces their number significantly.     

Fluorescein staining of human lymphocytes induced byEchis colorata venom

Résumé Le venin de l'Echis colorata produit la fluorescence des lymphocytes humains dans un mélange contenant du plasma défibrinogéné et de la fluorescéine. L'effet du venin est causé par l'action conjointe du thrombin produit par le procoagulant du venin et d'un autre facteur, qui peut être une protéase.

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This study was supported by a grant from the Israel Cancer Association.     

Fluorescein staining of guinea-pig lymphocytes induced byEchis colorata venom

Résumé Les lymphocytes de cobayes inoculés avec du venin de serpentEchis colorata, additionné de fluorescéinc, acquièrent une forte fluorescence. Le même effet du venin est observé avec des lymphocytes traités in vitro. Les fractions protéolytiques du venin sont responsables de ce phénomène, alors que la phospholipasc est sans effet.     

A staining pool for mammalian eggs

Zusammenfassung Beschreibung und Handhabung eines Färbetrichters mit basaler Abflussvorrichtung zur Durchführung histochemischer Reaktionen an Eizellen und frühen Furchungsstadien von Säugern. Der Färbetrichter ermöglicht ein mehrmaliges Wechseln der Lösungen ohne die Eier anzurühren, wodurch ein mehrmaliges Pipettieren der Eier vermieden werden kann.     

A differential staining for hypocotyl and radicle

Résumé Dans les études où la distinction entre l'hypocotyle et le radicule est nécessaire, leréactif genevois s'est montré le colorant le plus approprié.     

A modified vital staining technique for amphibian eggs

Zusammenfassung Es wird eine Methode beschrieben, die es ermöglicht, mit Hilfe eines gefärbten Agarfilms, der durch Löcher in Stanniolpapier gepresst wird, am Amphibien-Ei regelmässige Reihen von Vitalfarbmarken zu setzen.     

A method for selective staining of damaged yeast cells

Zusammenfassung Es wird eine neue Methode zur selektiven Färbung beschädigter (toter) Hefezellen mit Hilfe eines Gemischs von Uranylnitrat und Ponceau-Rot beschrieben, die grosse praktische Vorteile gegenüber der klassischen Methylenblau- und Nilblaufärbung besitzt.     

A mouse system for demonstrating the presence of inflammatory factors from human peripheral blood lymphocytes

Summary An assay system is described that allows the presence of inflammatory factors in supernatants from stimulated tuberculin-sensitive human peripheral blood lymphocytes to be demonstrated, by the induction of an inflammatory exudate in the peritoneal cavity of normal C57 BL mice.     

Chromosome breaking activity of A139 in human lymphocytes in vitro

Zusammenfassung 2,5-bis-(methoxyäthoxy)-3,6-bis-äthylenimino-p-benzochinon (Bayer A 139) induziert dosisabhängig Chromatidenaberrationen in menschlichen Lymphozyten in vitro. Die intrachromosomale Aberrationsverteilung ist nicht zufällig.

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I thank Mrs.R. Pieper for her careful technical assistance. I am also grateful to the firm Bayer, Leverkusen, for supplying the A 139.     

Receptors for cytomegaloviruses on the surface of human lymphocytes]

The relationships between cytomegalovirus (CMV) and lymphocytes have already been noted because of: (a) the immunological abnormalities induced by this virus, and (b) activation of latent CMV in the course of lymphocyte reactions associated with anti-histocompatibility antigen immune response. The present work shows that the lymphocyte surface may have specific receptors for CMV. Cultured fibroblasts infected with DMV were incubated with lymphocytes isolated from the blood of human immune subjects. Rosettes defined by the adherence of three or more lymphocytes around a fibroblast were formed in infected preparation while no rosettes were seen with normal control fibroblasts. Approximately 1.2 per cent of lymphocytes were involved in the formation of these rosettes. Rosette formation is inhibited when infected fibroblasts have been incubated with anti-CMV antibodies prior to the addition of lymphocytes.     

Peculiar ruthenium red staining pattern of the human early trophoblast

Summary This paper describes the ruthenium red staining (RRS) pattern observed on the 2 sides, maternal and fetal, of the human early trophoblast. The possibility of a different plasma membrane composition of the 2 sides of the human early trophoblast is discussed.