Histochemical study of dissociated nerve cells from embryonic chick cerebral hemispheres in flask cultures

Zusammenfassung Die Differenzierung von dissoziierten Grosshirnneuronen in Plastikflaschenkulturen wurde mit histochemischen Methoden, Nisslfärbung, Silberimprägnation der Nervenfasern und Acetylcholinesteraseaktivität untersucht.

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with the technical assistance ofM. F. Knoetgen

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Chargée de Recherche au C.N.R.S.     

Differentiation of dissociated neurons from chick embryo cerebral hemispheres cultivated inRose chambers with and without cellophane membrane

Zusammenfassung Dissoziierte Nervenzellen der Gehirnhemisphären von Hühnerembryonen wurden inRose-Kammern mit und ohne Cellophanmembran kultiviert. Die Differenzierung der Neurone wurde mit Phasenkontrast und histochemischen Methoden untersucht.

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Chargée de Recherche au CNRS.     

Effects of dibutyryl cyclic AMP on cultured brain cells from chick embryos of different ages.

The effect of dibutyryl cyclic AMP on cultivated astroblasts from 8-, 12- and 15-day-old chick embryo brain has been studied. The results show that these cells must have reached some degree of maturation in ovo to become morphologically differentiated by the cyclic nucleotide.     

Effects of dibutyryl cyclic AMP on cultured brain cells from chick embryos of different ages

Summary The effect of dibutyryl cyclic AMP on cultivated astroblasts from 8-, 12- and 15-day-old chick embryo brain has been studied. The results show that these cells must have reached some degree of maturation in ovo to become morphologically differentiated by the cyclic nucleotide.     

The effect of pulsating electrical current on isolated nerve cells from the chick cerebral hemispheres cultivated in the rose chamber

Summary It was shown that 73% of the fusiform cells obtained in short-term cultures of dissociated cerebral hemispheres of the chick embryo become oriented under the effect of a pulsating current, applied during the first 24 h of culture.Maître de Recherche au CNRS     

Development of nucleoside phosphotransferase activity in the cerebral hemispheres of embryonal and adult chick

Summary In the cerebral hemispheres of the chick embryo, the level of nucleoside phosphotransferase activity is much higher than that of thymidine kinase and it increases progressively during development up to the adult stage. Therefore nucleoside phosphotransferase is not coupled with DNA synthesis.     

The effect of concanavalin-A on the reaggregation of cells dissociated from Xenopus laevis early embryos

Summary The effects of concanavalin-A on the reaggregation and sorting of cells from Xenopus laevis early embroys have been studied. The results suggest that at high concentrations, concanavalin-A can prevent reaggregation.     

Ultrastructural response of the neural plate cells of chick embryos to dithiodiglycol

Résumé La neurulation a été inhibée chez des embryons de poulet par des traitements au dithiodiglycol (10^–3 M). L'analyse ultrastructurale révèle que seuls les microtubules subissent des modifications.     

Antigenic variation of the Avian Myeloblastosis Virus obtained from chick embryo fibroblasts

Zusammenfassung Immunelektrophoretisch wird ein verschiedenes Verhalten des Vogel-Myeblastosis-Virus nachgewiesen, je nachdem, ob dieses in Myeloblasten oder in Fibroblasten gezüchtet wurde.

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This work was done while the author was at Duke University Medical Centre, Durham (N.C, USA).     

Scanning electron microscopy study of spinal cord nerve cells from chick embryos cultured in vitro with poly-L-lysine]

Chick embryo nerve cells from the lumbo-sacral spinal cord have been isolated by trypsinisation and cultivated in Rose chambers on polylysine-L as a substrate. The cells are analysed by scanning electron microscopy. The mode of adherence of these cells to the substrate and the modifications of the surface of the neuroblasts during their transformation into neurocytes are studied.     

Mitotic activity of adrenal medullary cells in the mouse at different ages and following unilateral adrenalectomy.

The number of mitoses as well as the number of the cells of the adrenal medulla was determined in the mouse at various ages and after unilateral adrenalectomy. It was found that a decrease in the mitoses and an increase in the cell number occurs up to 4 months. In 12-month-old rats, mitoses, although rare, are still present. No changes in the mitosis and cell number was observed after unilateral adrenalectomy.     

Development of digits from the proximal pre-axial material of the wing and hind-limb bud in chick embryos

Résumé De nouvelles données expérimentales semblent confirmer l'hypothèse que chez l'embryon de poulet le développement des extrémités peut s'effectuer indépendamment de l'influence inductrice de l'épaississement apical de l'ectoderme.