A new method for determination of α-amylase

Zusammenfassung Für die Bestimmung der enzymatischen Aktivität der-Amylase wurde ein neues Substrat synthetisiert. Die Synthese kam in der Hauptsache durch die Farbstoffbindung an wasserlösliche Stärke und die anschliessende Vernetzung sowie durch den Zusatz von Diepoxid zustande.     

An isotope dilution method for the determination of dissolved carbon dioxide

Riassunto Si descrive un metodo per determinare il contenuto di anidride carbonica dell'acqua, che prevede il passaggio del CO₂ dall'acqua attraverso una membrana di lattice ad una soluzione alcalina dove la variazione di conduttività e radioattività viene misurata e usata per calcolare il livello di CO₂ del campione d'acqua.     

A rapid,sensitive method for the determination of follicle-stimulating activity

Riassunto Gli Autori riportano un nuovo metodo di saggio sul topo di piccole quantità di ormone follicolo stimolante (FSH), basato sulla riduzione intravaginale del cloruro di 2,3,5-trifeniltetrazolio cloruro (TTC). È cosi possibile determinare quantitativamente in sole 30 h dosi di FSH almeno 50 volte inferiori a quelle valutabili con i metodi fino ad oggi proposti.     

A spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate

Summary A new, sensitive, specific and simple spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate (PRPP) is presented. PRPP is reacted with excess hypoxanthine in the presence of hypoxanthine-guanine phosphoribosyltransferase. At the end of the reaction, PRPP concentration is measured from the extent of conversion of hypoxanthine to inositate. The concentration of the purine base is determined spectrophotometrically in the presence of xanthine oxidase.     

A rapid method for the purification of octopine dehydrogenase for the determination of cell metabolites

Summary Unlike other NAD⁺-dependent dehydrogenases, octopine dehydrogenase was not bound by blue Sepharose. A rapid 2-step purification procedure (gel filtration on Sephadex G-100 followed by affinity chromatography on blue Sepharose) resulted in a final preparation of octopine dehydrogenase which had a sp. act. of 65 units/mg protein and was free of contaminating NAD⁺-oxidoreductases. This preparation has been used successfully for the estimation of phospho-L-arginine, L-arginine and octopine in perchloric acid extracts.Supported by a grant from the Deutsche Forschungsgemeinschaft (Ga 241/1).     

A method for the determination of thrombocyte aggregation in circulating rat blood

Zusammenfassung Das Ausmass einer experimentell induzierten Thrombozytenaggregation im strömenden Blut wird kontinuierlich bestimmt durch Messung des Blutdrucks vor und hinter einem extrakorporal in den arteriellen Kreislauf der Ratte aufgenommenen Filter. Die Aggregation wird durch Infusion von Adenosindiphosphorsäure (ADP) vor dem Filter induziert. Die Aggregate schliessen das Filter teilweise ab, was zu Änderungen im Blutdruck vor und hinter dem Filter führt. Aus diesen Änderungen lässt sich das Mass der Aggregation ermitteln. Es wurde gefunden, dass dieses durch die ADP-Gabe bedingt wird.     

A spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate.

A new, sensitive, specific and simple spectrophotometric method for the determination of 5-phosphoribosyl-l-pyrophosphate (PRPP) is presented. PRPP is reacted with excess hypoxanthine in the presence of hypoxanthine-guanine phosphoribosyltransferase. At the end of the reaction, PRPP concentration is measured from the extent of conversion of hypoxanthine to inositate. The concentration of the purine base is determined spectrophotometrically in the presence of xanthine oxidase.     

A rapid HPLC method for determination of adenylate energy charge

A simple procedure is described for separation and analysis of adenine nucleotides in tissue extracts, utilizing anion exchange HPLC. Determination of AMP, ADP, and ATP takes 10 min per sample.     

A simple method for the estimation of yeast-growth in hydrocarbon-substrates by determination of turbidity

Zusammenfassung Es wird eine cinfache und schnelle Methode zur Bestimmung der Zellkonzentration in kohlenwasserstoffhaltigen Kulturflüssigkeiten beschrieben. Zur Abtrennung der Kohlenwasserstoffe wird die Probe nach Zusatz von Teepol zentrifugiert, danach die Zellkonzentration durch Trübungsmessung bestimmt.     

Depression of the isolated guinea-pig uterus by reserpine

Zusammenfassung Reserpin verhindert sofort die elektrische Reizbarkeit des isolierten Meerschweinchenuterus. Eine Depression wurde von einigen Substanzen nur partiell bewirkt, wobei sich auffallende Wirkungsunterschiede zeigten.