Overexpressions of Lambda Phage Lysis Genes and Biosynthetic Genes of Poly-β-hydroxybutyrate in Recombinant E.coli

ＩｎｔｒｏｄｕｃｔｉｏｎＰｏｌｙβｈｙｄｒｏｘｙｂｕｔｙｒａｔｅ（ＰＨＢ）ｉｓａｋｉｎｄｏｆｂｉｏｄｅｇｒａｄａｂｌｅｐｌａｓｔｉｃｓａｃｃｕｍｕｌａｔｅｄａｓｇｒａｎｕｌｅｓｉｎｃｅｌｓ．Ｔｈｅｆｉｒｓｔｓｔｅｐｔｏｓｅｐａｒａｔｅｉｔｉｓｃｅ...     

Evolutionary Mode of Metallothioneins Inferred from Cd-MT Genes of Tetrahymena

From Tetrahymena thermophila （strain BF5）, the coding region of Cd-MT gene was cloned and sequenced. and identified as MTT1 isoform. A serial duplicate structure is discovered in its amino acid sequence, which separates the coding region into three parts （Part 1：7-61; Part 2：64-118; Part 3：122-162）. The alignments among them and comparison with the corresponding parts of MT1 isoform suggest that MT1 and MTT1 isoforms both come from the same ancient gene that is homologous to Part 1, and Cd-MTs of Tetrahymena are aroused by such ancient gene duplication. The prediction of secondary structures and the analysis of the disulfide-bonding state of cysteine show that there are a lot of differences between MT1 and MTT1 isoforms, which maybe relate to their function mechanism.     

Transformation of Streptococcus zooepidemicus with Genes Responsible for Polyhydroxybutyrate Synthesis

IntroductionGenetic manipulation of bacteria has become apowerful tool for elucidating fundamentalbiological mechanisms.While many techniques areavailable to introduce foreign DNA into severalbacterial species[1 ] ,some bacteria,such asStreptococcus spp.,have proven to be resistant totransformation with mostprotocols thathave beendescribed.In addition,there are large differencesin the transform ability among various strains ofStreptococcusspp.　Streptococci have been divided into six majorph…     

Exploring Codon Usage Patterns of Alternatively Spliced Genes in Human Chromosome 1

Introduction A large number of species (prokaryotes and eukaryotes) have been used to study the pattern of codon usage bias, and it has also been demonstrated that inter- and intra-genomic variation of the pattern of codon usage is a widespread phenomenon, which may result from various factors. Alternative synonymous codon usage does not modify the amino acid sequence encoded in DNA among species and often among genes from the same genome. It has been suggested that the pattern of codon usag…     

Microarray-Based Differential Expression Monitoring of 79 Novel Genes in Human Fetal Tissues

0　ＩｎｔｒｏｄｕｃｔｉｏｎＬａｒｇｅ ｓｃａｌｅｓｅｑｕｅｎｃｉｎｇａｎｄｔｈｅａｐｐｌｉｃａｔｉｏｎｏｆｅｘｐｒｅｓｓｅｄｓｅｑｕｅｎｃｅｔａｇ(ＥＳＴ)ｔｅｃｈｎｏｌｏｇｙｈａｓｌｅｄｔｏｔｈｅｉｄｅｎｔｉｆｉｃａｔｉｏｎｏｆｈｕｎｄｒｅｄｓａｎｄｔｈｏｕｓａｎｄｓｏｆｇｅｎｏｍｉｃｓｅｑｕｅｎｃｅｓｆｒｏｍｖａｒｉｏｕｓｍｉｃｒｏｂｅｓｔｏｍｕｌｔｉｃｅｌｌｕｌａｒｅｕｋａｒｙｏｔｅｓａｎｄｐｌａｎｔｓ[1,2 ] .ＥｘｐｒｅｓｓｉｏｎａｎａｌｙｓｉｓｏｆｅａｃｈｋｎｏｗｎｇｅｎｅｏｒＥＳＴｕｓｉ…     

Cytogenetic Mapping of Disease Resistance Genes and Analysis of Their Distribution Features on Chromosomes in Maize

0　IntroductionMaizeisamongthemostintensivelystudiedspeciesingeneticsandoneofagronomicallythemostimportantplants.Therearemanydis easemicrobesandpeststoattackmaize,whichre sultsinlowproductionandbadquality .Withthedevelopmentofverydensegeneticmapconstruc tion ,avarietyoftheimportantdiseaseresistancegenesofmaizeincludingHelminthosporiumtur ciumPassresistancegenesHt1,Htn1andHt2 ,HelminthosporiummaydisNisikresistancegenesRhm1andRhm2 ,maizedwarfmosaicvirusresis tancegeneMdm1,wheatstreakmosaicvi…     

A Distorted Image of Confucius ——on Lin Yutang′s Version of Lunyu(论语)

ＡｍｏｎｇｍｏｄｅｒｎＣｈｉｎｅｓｅｗｒｉｔｅｒｓ，ＧｕＨｏｎｇｍｉｎｇ（辜鸿铭）ａｎｄＬｉｎＹｕｔａｎｇ（林语堂）ａｒｅｔｈｅｔｗｏｍｏｓｔｐｒｏｌｉｆｉｃｔｒａｎｓｌａｔｏｒｓｏｆＣｈｉｎｅｓｅｌｉｔｅｒａｔｕｒｅ．ＧｕＨｏｎｇｍｉｎｇ，ｔｈｅｗｒｉｔｅｒｏｆＱｉｎｇＤｙｎａｓｔｙ，ｈａｓａｌｍｏｓｔｂｅｅｎｆｏｒｇｏｔｔｅｎｂｙｏｒｄｉｎａｒｙＣｈｉｎｅｓｅｒｅａｄｅｒｓａｔＰｒｅｓｅｎｔ；ｗｈｉｌｅＬｉｎＹｕｔａｎｇｉｓｅｖｅｒｆｒｅｓｈａｎｄｎｅｗｔｏｐｌｅｎｔｙｏｆｒｅａｄｅｒｓ—…     

Analysis of Type Ⅱ Toxin-Antitoxin Genes all 3211-asl3212 in Anabaena PCC 7120

The type Ⅱ toxin-antitoxin genes are responsible for the phenotypic switch to a quasi-dormant state that enables cell survival under stresses,a similar function to heterocyst of cyanobacteria. In this paper,we particularly study the role of gene pair all3211-asl3212 under Spectinomycin stress to reveal how the type Ⅱ toxin-antitoxin involved in environmental stress responses. Bioinformatics prediction shows that toxin protein gene All3211 is homologous to Maz F,a member of maz EF family that encoding nucleases. We clone gene all3211-asl3212 into expression vectors to identify its molecular characteristics. Deletion mutant strains of all3211-asl3212 are selected in a tri-parental mating screen. Phenotype comparisons of mutant and wild type reveals no difference of single-deletion-mutants in pigment integrity,the sensitivity to antibiotics,and heterocyst formation. The results show that deletion mutation of single TAS gene pair all3211-asl3212 results in limited effects on the cellular growth of PCC 7120. Thus,we suggest that dosage compensating might be provided from redundant genes or bypass pathways to offset obvious phenotypic differences.     

Application of EEG multipole model in the diagnosis of Alzheimer’s disease

In order to find the electroencephalogram (EEG) characteristic parameters typical of Alzheimer’s disease (AD) and explore an effective diagnostic method, a new mobile current multipole model was proposed to simulate the AD patient’s cortical dipole source activities. The indicators of goodness of fit (GOF) and DtononD (DD) were calculated from EEG samples to evaluate the performance of this model. Relevant results showed that this multipole model with higher GOF values and larger DD change well fitted the pathological electrical activities of cortical neurons aroused by AD’s extended sulcus and gyrus in the cerebral cortex. Meanwhile, the products of DD mean & standard variance were found in a clear linear correlation with the diagnostic data of mini-mental state examination (MMSE) used in AD clinics. Furthermore, by tracing this multipole model’s indicators in typical patients and contrasting with the functional magnetic resonance image (fMRI) as AD progressed, we suggested that the DD index may be suitable for monitoring the AD developments as a new diagnostic parameter.     

Application of EEG multipole model in the diagnosis of Alzheimer’s disease

In order to find the electroencephalogram (EEG) characteristic parameters typical of Alzheimer’s disease (AD) and explore an effective diagnostic method, a new mobile current multipole model was proposed to simulate the AD patient’s cortical dipole source activities. The indicators of goodness of fit (GOF) and DtononD (DD) were calculated from EEG samples to evaluate the performance of this model. Relevant results showed that this multipole model with higher GOF values and larger DD change well fitted the pathological electrical activities of cortical neurons aroused by AD’s extended sulcus and gyrus in the cerebral cortex. Meanwhile, the products of DD mean & standard variance were found in a clear linear correlation with the diagnostic data of mini-mental state examination (MMSE) used in AD clinics. Furthermore, by tracing this multipole model’s indicators in typical patients and contrasting with the functional magnetic resonance image (fMRI) as AD progressed, we suggested that the DD index may be suitable for monitoring the AD developments as a new diagnostic parameter.     

Expressions of Two Rice Mip Genes under Water Stres,Salt Stress and Exogenous ABA

ＥｘｐｒｅｓｉｏｎｓｏｆＴｗｏＲｉｃｅＭｉｐＧｅｎｅｓｕｎｄｅｒＷａｔｅｒＳｔｒｅｓ，ＳａｌｔＳｔｒｅｓａｎｄＥｘｏｇｅｎｏｕｓＡＢＡ＊ＺｈｏｕＺｈｉｑｉ（周志琦），ＬｉｕＱｉａｎｇ（刘强），ＫａｚｕｋｏＹａｍａｇｕｃｈｉＳｈｉｎｏｚａｋｉ＋，Ｈｉｒ...     

inite Element Analysis of Capaci

of Civil Engineering,Zhengzhou University,Zhengzhou 450002,China)The bearing capacity is one of the most important perfo     

Performance Comparison of Neural Networks for HRTFs Approximation

In order to approach to head-related transfer functions (HRTFs), this paper employs and compares three kinds of one-input neural network models, namely, multi-layer perceptron (MLP) networks, radial basis function (RBF) networks and wavelet neural networks (WNN) so as to select the best network model for further HRTFs approximation. Experimental results demonstrate that wavelet neural networks are more efficient and useful.     

Calculation of the Neutron Direct Inelastic Scattering cross Section of ~ (238) U

ＣａｌｃｕｌａｔｉｏｎｏｆｔｈｅＮｅｕｔｒｏｎＤｉｒｅｃｔＩｎｅｌａｓｔｉｃＳｃａｔｅｒｉｎｇｃｒｏｓＳｅｃｔｉｏｎｏｆ２３８Ｕ＊ＣｈｅｎＺｈｅｎｐｅｎｇ（陈振鹏），ＲｅｎＪｉｅ（任杰），ＺｈｕＱｉｎｇｙｕａｎ（祝清源）ＤｅｐａｒｔｍｅｎｔｏｆＭｏｄ...     

Production of Biodegradable Copolyesters and Terpolyesters of Polyhydroxyalkanoates by Alcaligenes latus DSM 1124

ＰｒｏｄｕｃｔｉｏｎｏｆＢｉｏｄｅｇｒａｄａｂｌｅＣｏｐｏｌｙｅｓｔｅｒｓａｎｄＴｅｒｐｏｌｙｅｓｔｅｒｓｏｆＰｏｌｙｈｙｄｒｏｘｙａｌｋａｎｏａｔｅｓｂｙＡｌｃａｌｉｇｅｎｅｓｌａｔｕｓＤＳＭ１１２４ＣｈｅｎＧｕｏｑｉａｎｇ（陈国强），ＣｈｅｎＪｉ...     

One-step disposal of Cr (Ⅵ)-bearing wastewater by natural pyrrhotite

Cr(Ⅵ)-bearing wastewater can be treated by natural pyrrhotite which is used for reductant to reduce Cr(Ⅵ) and precipitant to precipitate Cr(Ⅲ) simultaneously. The disposal products can be divided into three parts in the beakers,namely supernatant in the upper part,the yellowish colloidal precipitates in the middle part and the pyrrhotite in the lower part. The content of total Cr=Cr(Ⅵ)+Cr(Ⅲ) in the supernatant liquid is 0.06 mg/L,which is lower than 1.5 mg/L of the discharge standard of China and near to 0.05 mg/L of the standard of potable water. This one-step disposal composing of both reduction and precipitation which is traditionally divided into two independent steps called reducing technology and precipitating technology respectively. The new method is of obvious economic advantage and favourable to decreasing surplus mud derived from adding Ca(OH)2 to precipitate Cr(Ⅲ) traditionally so as to avoid recontamination. In fact,sodium sulfite (Na2SO3) used in disposal of Cr(Ⅵ) was traditionally produced from natural mineral of pyrrhotite (FeS). One molecule of FeS is 4 times more than that of Na2SO3 from a view point of rational use of mineral resources. Therefore the prospective of application of the one-step disposal of Cr(Ⅵ) method is full of promise.     

Flame retardancy of polyamide 6 hybrid fibers:Combined effects ofα-zirconium phosphate and ammonium sulfamate

Synergistic effect between α-zirconium phosphate(α-ZrP) and ammonium sulfamate(AS) for enhanced flame retardant properties of Polyamide 6(PA6) was investigated by using oxygen index instrument,cone calorimeter,thermogravimetric analyzer(TGA),Instron universal test machine and scanning electron microscopy(SEM).PA6/AS/α-ZrP ternary hybrid materials with various contents of α-ZrP and AS were fabricated by melt-mixing method.The result from flammabihty indicated that the Limiting oxygen index(LOI) and Underwriters Laboratories-94(UL-94) rating of PA6/AS/α-ZrP were significantly accelerated under the coordinating function of α-ZrP and AS.Moreover,the thermal stability for PA6/AS/α-ZrP studied by TGA also demonstrated this synergistic effect between α-ZrP and AS on the heat resistance.The effects of the usage amount of α-ZrP and AS on mechanical properties were analyzed by using uniaxial tensile test.It was found that the addition of AS provided negative effects on the tensile strength of PA6/AS/α-ZrP,however,the adverse trends that mentioned above could be overcome by using the well dispersed α-ZrP.     

Two-dimensional investigation of forced bubble oscillation under microgravity

Recent referential studies of fluid interfaces subjected to small vibration under microgravity conditions are reviewed. An experimental investigation was carried out aboard the American Space Shuttle Discovery. Two-dimensional (2-D) modeling and simulation were conducted to further understand the experimental results. The oscillation of a bubble in fluid under surface tension is governed by the incompressible Navier-Stokes equations. The SIMPLEC algorithm was used to solve the partial differential equations on an Eulerian mesh in a 2-D coordinate. Free surfaces were represented with the volume of fluid (VOF) obtained by solving a kinematic equation. Surface tension was modeled via a continuous surface force (CSF) algorithm that ensures robustness and accuracy. A new surface reconstruction scheme, alternative phase integration (API) scheme, was adopted to solve the kinematic equation, and was compared with referential schemes. Numerical computations were conducted to simulate the transient behavior of an oscillating gas bubble in mineral oil under different conditions. The bubble positions and shapes under different external vibrations were obtained numerically. The computed bubble oscillation amplitudes were compared with experimental data.     

Calculation of Three-dimensional Electrostatic Field in Potential Transformers

Ｉｔｉｓｏｆｇｒｅａｔｓｉｇｎｉｆｉｃａｎｃｅｔｏｓｉｍｕｌａｔｅｔｈｅｅｌｅｃｔｒｏｓｔａｔｉｃｆｉｅｌｄｉｎｈｉｇｈｖｏｌｔａｇｅｐｏｗｅｒａｐｐａｒａｔｕｓｅｓｗｈｉｃｈｃａｎｉｍｐｒｏｖｅｔｈｅｉｒｓｔｒｕｃｔｕｒｅｓ．Ａｌｔｈｏｕｇｈｔｈｅｒｅｉｓｎｏｓｐｅｃｉａｌｐｒｏｂｌｅｍｉｎｔｈｅｓｉｍｕｌａｔｉｏｎｏｆｅｌｅｃｔｒｏｓｔａｔｉｃｆｉｅｌｄｓ，ｄｉｆｆｉｃｕｌｔｉｅｓｓｔｉｌｌｒｅｍａｉｎｉｎｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆｔｈｒｅｅｄｉｍｅｎｓｉｏｎａｌ（３Ｄ）ｓｏｆｔｗ…