Thein vitro effect of various enzymes upon the mouse ascites tumor of ehrlich

Zusammenfassung Die Virulenz von Ehrlich-Ascitestumorzellen wurde durch Inkubation mit fünf Enzymenin vitro stark herabgesetzt. Zwölf andere Enzyme hatten keine Wirkung. Die morphologischen Kriterien für Zellschädigung werden erörtert.

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This work was supported in part by a grant from the American Cancer Society (Mass. Division) and in part by grants (C-3538 and CY-4018) from the U.S. Public Health Service, National Cancer Institute.     

Action of sugars on methionine transfer in ehrlich ascites carcinoma cells

Zusammenfassung Bei mit Zucker aufgeladenen Ehrlich-Ascites-Tumorzellen steigert sich das Eindringen des Methionins in die Zelle. Wenn sich dieselben Zucker im extrazellulären Raum befinden, bleibt dieser Effekt aus. Die möglichen Ursachen dieses Effektes werden besprochen.     

Isolation from cobra venom of a factor inhibiting glycolysis in ehrlich ascites carcinoma cells

Résumé Un inhibiteur de la glycolyse anaérobie est purifié à partir du venin deNaja naja atra par un procédé couplant la chromatographie sur SE Sephadex et la filtration sur gel moléculaire. L'inhibition complète de la glycolyse anaérobie est obtenue sur une suspension cellulaire en 60 min pour une concentration inférieure à 20 µg/ml du produit purifié. L'inhibiteur est de nature protéinique et ne présente aucune activité nucléotidasique ni lécithinasique A.     

The influence of dimethyl sulphoxide on metabolic activity of ehrlich ascites carcinoma cells and microorganisms

Zusammenfassung Es wird gezeigt, dass 1–2%iges Dimethylsulfoxyd sich als unschädliches Lösungsmittel für antibakterielle Substanzen besonders gut eignet.     

Glycerin prevents the ‘heat-death’ of ehrlich ascites tumor cells

Summary When heated to 45°C for 20 min Ehrlich ascites tumor and NK-lymphoma cells are irreversibly damaged, so that they lose transplantability. The presence of 7–8% glycerol during heat treatment protects EAT cells completely and NK-lymphoma cells partly against this injury.     

In vitro anti-tumor activity of lipid extracts from a group aStreptococcus against ehrlich ascites carcinoma in mice

Résumé On a isolé chez la souris, d'une souche deStreptococcus hemolyticus une fraction lipidique qui inhibe complètement le développement de la tumeur ascitique d'Ehrlich quand la fraction lipidique est préincubée avec les cellules de la tumeur avant inoculation.     

Effect of magnesium pemoline on the survival of mice with ehrlich ascites tumor

Zusammenfassung Magnesium Pemolin, ein zentralnervöses Reiz- und gutes Schutzmittel gegen Radiumbestrahlung, verlängert die Lebensdauer von Ehrlich-Ascites-Tumor-Mäusen, und zwar mit oder ohne zusätzlichen Einfluss einer Dosis nichttödlicher Bestrahlung (500 R).

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This work was supported in part by grant No. 2-44-33-90-3-06 from the Norval H. Pierce Research Fund, University of Illinois and in part by a grant from Abbott Laboratories, North Chicago.     

Organ weights of TE mice bearing ehrlich ascites carcinoma growing in the solid form and of C3H mice bearing spontaneous mammary carcinoma

Zusammenfassung Das Milzgewicht karzinomtragender Mäuse ist dreimal so hoch wie das Milzgewicht gesunder Kontrollen.Die Leber karzinomtragender Mäuse ist ungefähr anderthalbmal so schwer wie die Leber gesunder Kontrollen.Das Nierengewicht von C3H-Mäusen mit Mammakarzinom beträgt nur etwas mehr als die Hälfte des Nierengewichtes gesunder Kontrollen.Intraperitoneale Injektion von 0,25 ml Vollblut oder 0,2 ml Plasma von TE-Mäusen mit Ehrlich-Ascites-Karzinom in der solid wachsenden Form erhöht das Milzgewicht gesunder Kontrollen auf das Doppelte.     

Metabolism of different histone fractions in ehrlich ascites cells after environmental change

Zusammenfassung In Ehrlich-Asziteszellen, die ungünstigen Umweltbedingungen ausgesetzt sind, hört die Zellteilung auf. Doch auch ohne DNS-Synthese geht der Histon-Stoffwechsel noch eine Zeitlang weiter, wobei die Synthese der argininreichen Histone dreimal so gross ist wie die der anderen Typen. Diese von der Zellteilung unabhängige Histon-Synthese ist ein Teil des Mechanismus, mit welchem die Zellen versuchen, sich an die neuen ungünstigen Nahrungsbedingungen anzupassen.

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This investigation was supported by a grant from The Robert A. Welch Foundation No. H 393. The author wishes to acknowledge the competent assistance of Mrs.Katherine Servanse in connection with the measurements of the specific activities of histone fractions.     

Photoinactivation of ehrlich ascites tumor cells in vitro obtained with skin-photosensitizing furocoumarins

Riassunto Il liquido ascitico del tumore di Ehrlich, irradiato a 3655 Å in presenza di furocumarine «fotosensibilizzatrici cutanee» e cioè di psoralene o xantotoxina o bergaptene, perde la capacità di trasmettere il tumore nel topo.     

Effects of retinoic acid on ascites cells of the TA3 mouse mammary carcinoma

Summary Retinoic acid caused a decrease in adhesiveness but no growth change in the allotransplantable TA3-Ha cell and no change in adhesiveness or growth in the strain specific TA3-St cell. The retinoic acid binding protein was detected in the TA3-Ha, but not the TA3-St, cell.This study was supported in pat by Public Health Service Grants CA-08418 and CA-18600 from the National Cancer Institute, National Institutes of Health. This is publication 955 of the Robert W. Lovett Group for the Study of Diseases Causing Deformities. We gratefully acknowledge the contributions of Drs Luigi M. De Luca and Anton M. Jetten of the Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, National Institutes of Health, Bethesda, MD, without whose help this study would not have been possible.     

Effects of retinoic acid on ascites cells of the TA3 mouse mammary carcinoma

Retinoic acid caused a decrease in adhesiveness but no growth change in the allotransplantable TA3-Ha cell and no change in adhesiveness or growth in the strain specific TA3-St cell. The retinoic acid binding protein was detected in the TA3-Ha, but not the TA3-St, cell.     

Surface morphological study of ehrlich ascites tumor cells exposed to microware irradiation and heat

Summary Microwave irradiation of EAT cells caused an increase in length and number of surface microvilli. The tumor cells tend to form large aggregates by means of extensive interdigitation of surface microvilli. On the other hand, heat hyperthermia caused a decrease of surface microvilli but an increase of surface blebs. Hence the surface morphology of EAT cells after in vitro exposure to microwave irradiation differs markedly from that after heat hyperthermia.Part of the results was presented a the Fifth meeting of the European Co-operative Hyperthermia Group, Essen, July 1983.     

Studies on the kinetic of alkaline phosphatase reaction in ehrlich ascites tumour cells with the use of interference microscopy

Zusammenfassung Mittels Interferenzmikroskopie wurde die Präzipitationsrate des Calciumphosphats in Ehrlich Ascites-Tumorzellen gemessen und die Beeinflussung der alkalischen Phosphataseaktivität durch diverse Faktoren untersucht.     

Isolation of a pharmacologically active principle from ehrlich carcinoma tumor cells

Résumé Les propriétés biologiques d'une nouvelle substance pharmacologique active isolée des cellules de l'ascite d'Ehrlich sont décrites en détail. La substance isolée contracte in vitro l'iléum du cobaye ou l'utérus du rat et relaxe l'ileum de la souris. In vivo, cette substance possède un effet hypotensif chez le chat.     

In vivo effect of sodium valproate on mouse liver

The in vivo effect of sodium valproate (SV) on the activity of uridine diphosphate glucuronosyltransferase (UDP-GT) and hepatotoxicity in the mouse liver was studied. Mice were injected intraperitoneally (IP) with SV at doses varying from 50 to 800 mg/kg per day, for six consecutive days (dose-response group) or at a standard dose of 300 mg/g per day for 2-10 days (time-response group), whereas the controls were injected with normal saline. Valproic acid levels had a positive correlation to the dose (P < 0.001) and duration of drug administration (P = 0.006). A gradual increase in UDP-GT activity was observed in doses of up to approximately 400 mg/kg per day, whereas in higher doses the enzyme activity gradually decreased. The time course of UDP-GT activity at the standard dose of 300 mg/kg per day increased progressively, with a maximum up to the sixth day and then had a gradual reduction. Hepatic necrosis (which was unrelated to the dose or the duration of drug administration) was found in 13% of the SV-treated animals and in none of the controls. We conclude that at an optimal dose (300-400 mg/kg per day) and at a time course of 6 days, SV causes liver UDP-GT induction, whereas in higher doses and longer duration of administration, UDP-GT activity is gradually reduced. SV also causes hepatotoxicity unrelated to dose and time course.