A major metabolite of Δ1-tetrahydrocannabinol reduces its cataleptic effect in mice

Summary The results described here demonstrate that THC-induced catalepsy in mice can be substantially inhibited by the prior administration of ¹-THC-7-oic acid, the major metabolite of THC in most species including humans. This raises the possibility that the intensity and duration of action of THC may depend to a large degree on the levels of this metabolite at the sites of action.We thank the National Institute on Drug Abuse for supporting this project by grants DA-02043 and DA-02052 and for supplying all of the cannabinoids. One of us (S.B.) is also the recipient of a Research Scientist Award from NIDA. We are grateful to Kristen Carlson and Thomas Honeyman for helpful suggestions in preparing this report.     

Epstein-barr virus and inflammatory bowel disease

Zusammenfassung Die Häufigkeit und der Antikörpertiter gegen Epstein-Barr-Virus (EBV) wurde in Patienten mit Ulkus-Colitis und Crohn's Krankheit, eine granulomatische Abnormalität ähnlich zur Sarkoidose untersucht und normale Häufigkeit und Antikörpertiter gegen EBV bei ihnen gefunden.

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Supported by Research Contract No. NIH-69-2078 within the Special Virus Cancer Program of the National Cancer Institute, National Institutes of Health, USPHS, National Institutes of Health Grant No. AM 05126 and the National Foundation for Ileitis and Colitis, Inc., New York, New York.

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Dr.Grotsky is a recipient of a National Institutes of Health Graduate Training Grant in Gastroenterology, No. Am 05126.

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Dr.Hirshaut is a recipient of a National Cancer Institute Grant No. CA 08748 an a grant from the New York Cancer Research Institute.

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Dr.Glade is a recipient of a Research Career Development Award No. A1-46371 of the USPHS.     

Changes in brain acetylcholinesterase activity of young rats after chronic treatment with tremorine

Résumé La trémorine a été administrée chez les rats quotidiennement pendant 7 jours à des doses de 7.5, 22.5 ou 30 mg/kg. Les témoins ont montré des mouvements convulsifs proportionnels à la dose de trémorine. Quatre jours après l'arrêt du traitement, l'activité acétylcholinestérasique du cortex cérébral était augmentée. Il est proposé que ce change de l'activité acétylcholinestérasique reflète une utilisation accrue de l'acétylcholine corticale.

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Acknowledgement: This investigation was supported by a U.S. Public Health Service Grant No. NS 09199 and a Research Scientist Development Award No. K02 MH-42479 from the National Institute of Mental Health, National Institutes of Health.

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The author gratefully acknowledges the technical assistance ofSandra Roland.     

Chloroperoxidase-catalyzed oxidation of N-methyl-4-chloroaniline

Summary Chloroperoxidase catalyzed the H₂O₂ oxidative conversion of N-methyl-4-chloroaniline to 4-chloronitrosobenzene, 4-chloroaniline and a mixture of complex products.This study was supported by grant No. CA 21992 from the National Cancer Institute, and by Research Career Development Award ES 00038 to M.D.C. from the National Institute of Environmental Health Sciences, DHEW.     

Influence of common bile duct cannula size on maximal secretory rate of taurocholate in the rat

Summary The common bile duct of male Sprague-Dawley rats was cannulated with either PE 10 or PE 50 tubing. Maximal secretory rate of taurocholate averaged 389±67 (SD) and 657±115 nmoles·min^–1·g liver^–1 in the PE 10 and PE 50 group, respectively (p<0.005). Maximal bile secretory pressure was significantly higher in the PE 10 group (240±28 vs 174±8 mm H20; p<0.005). When the maximal secretory rate was exceeded, bile flow decreased in both groups but this was accompanied with a decrease in maximal bile secretory pressure in the PE 10 group only. Maximal secretory rate of bile salts is markedly influenced by experimental technique. Use of small caliber common bile duct cannulae leads to partial obstruction and decreases the apparent maximal secretory rate for taurocholate.Acknowledgments. J. Reichen was the recipient of a Faculty Development Award in Clinical Pharmacology from The Pharmaceutical Manufacturer's Association Foundation, and is the recipient of a Research Career Development Award (KO4 AM 1189) from the National Institutes of Health. Supported by National Institutes of Health grant RO1 AM 27597.     

The generality of the nitroso-glyoxylate reaction: Conversion ofp-nitronitrosobenzene to the hydroxamic acid

Summary The reaction of glyoxylic acid withp-nitronitrosobenzene (1b) in dilute aqueous solution gave the hydroxamic acid (2b) as the major detectable product. The significance of this observation with respect to the title reaction is discussed.This study was supported by grant No. CA 32395 from the National Cancer Institute, and by Research Career Development Award ES 00120 from the National Institute of Environmental Health Sciences, DHHS.     

A simplified method for isocratic HPLC analysis of polyamines

Summary A simple technique is described for the separation and analysis of polyamines in tissues and body fluids, utilizing precolumn clean-up on disposable CM-cellulose columns, followed by an automatable HPLC procedure. Complete separation and analysis takes 12–15 min per sample with sensitivity in the pmole range.Acknowledgments. Supported by USPHS HD-09713, EPA R811621010 and by Research Scientist Development Award DA-00006 to TAS.     

sRNA methylase activity of embryonic liver

Résumé Utilisant SAM-¹⁴CH₃ il a été démontré que le foie foetal a plus d'activité de sRNA methylase que le foie adulte.

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This investigation was supported in part by an allocation from a Public Health Service General Research Support Grant No. SO 1 FR-05545-03, and an American Cancer Society Grant No. IN 19F to The Jackson Laboratory, Bar Harbor, Maine, and by Public Health Service Research Grants No. HD-01496 from the National Institute of Child Health and Human Development and No. FR-00251 from the Division of Research Facilities and Resources.     

An arachidonic acid-specific lipoxygenase from the gorgonian coralPseudoplexaura porosa

Summary A novel lipoxygenase was partially purified from the gorgonian coralPseudoplexaura porosa and was found to be specific for arachidonic acid. This soluble enzyme catalyzed the formation of 15-hydroperoxy-eicosatetraenoic acid (15-HPETE) from arachidonic acid.This investigation was supported by PHS grant No. CA32395 awarded by the National Cancer Institute and by PHS Research Carreer Development Award No. ES00120 to MDC awarded by the National Institute of Environmental Health Sciences, DHHS. The authors acknowledge Ms M. A. Russell and Dr D. G. Anderson of the University of Miami for assistance in the collection of gorgonian corals. Preliminary work was conducted at the University of Miami School of Marine and Atmospheric Science. This is journal series No. 3638 from the Florida Agricultural Experiment Station.     

Observations on the centromere area of human chromosomes

Zusammenfassung Das dem Centromer der menschlichen Chromosomen (ebenfalls bei andern Arten) anliegende Gebiet erscheint hell, klar und rund. Dies wurde in einigen Zellen bei fast jedem Centromer, bei andern Zellen nur selten oder überhaupt nicht beobachtet. Eine ähnliche Erscheinung trat auch in Form von Streifen auf, die sich manchmal vom Centromer aus zu anliegenden Chromosomen erstreckten oder sich ins Cytoplasma ausbreiteten. Die mögliche Deutung dieser Beobachtung wurde besprochen.

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This project was supported in part by Public Health Service Career Development Award No. K3-CA-19, 745 from the National Cancer Institute, Research Grant No. GM 11078 from the General Medical Science Branch, and the John A. Hartford Foundation.     

Cellular and subcellular localization of3H-diethylstilbestrol in the pituitary,oviduct and uterus of the female rat

Summary Female rats were injected with³H-diethylstilbestrol. The pituitaries, oviducts, and uteri were removed and processed for autoradiography. The nuclear uptake and retention in these tissues appeared similar to that seen after the injection of³H-estradiol except in the pars nervosa of the pituitary gland.This study was supported by US PHS Career Development Award No. NS00164 and PHS Grant No. NS21933.     

Involvement of circadian system in photoperiodic control of pubertal development in female deer mice,Peromyscus maniculatus

Summary Pubertal development in female deer mice,Peromyscus maniculatus, was stimulated by exposure to light cycles of LD 168, LD 630 or LD 654, but not by exposure to cycles of LD 618 or LD 642. These results support the hypothesis that female deer mice use a circadian rhythm of responsiveness to light to measure photoperiodic time.This investigation was supported in part by National Science Foundation Grant PCM 8116880 and U.S. Environmental Protection Agency Grant CR809428010. H.U. is the recipient of Research Ca-reer Development Award 5 KO4 HD00276-02 from the National Institute of Child Health and Human Development.     

A mechanical study of the cat retractor bulbi muscle

Zusammenfassung Die mechanischen Eigenschaften des M. retractor bulbi in der Katze (Anstiegs- und Abfallzeiten der Einzelzuckung und der tetanischen Kontraktion und Fusionsfrequenz) liegen zwischen den Eigenschaften der schnellen und langsamen Zuckungsfasern des M. obliquus inferior.

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This investigation was supported by Public Health Service Program Project Research Grant No. NB 06038, General Research Support Grant No. 5 S01 FR-05566, and Research Career Program Award No. K3-NB-14,094 to Dr.Bach-y-Rita from the National Institute of Neurological Diseases and Blindness.     

Moderate cooling depresses the accumulation and the release of newly synthesized catecholamines in isolated canine saphenous veins

Summary Moderate cooling (from 37° to 24°C) depressed the formation of³H-dopamine and³H-norepinephrine from³H-tyrosine by isolated canine saphenous veins. Cooling reduced the evoked release ofnewly synthesized catecholamine to the same extent as tha of stored norepinephrine. Hence the augmentation by cold of the contractile response to sympathetic nerve stimulation observed in earlier work is not accompanied by an augmentated release of newly synthesized norepinephrine.Acknowledgments. This work was supported by grant HL 05883 from the National Institutes of Health. The authors want to thank F. Jordaens for his valuable technical assistance, J. Beckman for secretarial assistance and H. Hedrickson for preparing the illustrations.Recipient of training grant 80304 from the I.W.O.N.L.     

Calcification of the parathyroids induced by Calciphylaxis

Résumé Une calcification des glandes parathyroïdes a été réalisée chez le rat par sensibilisation avec une dose orale unique de dihydrotachystérol suivie, le lendemain, d'une seule injection intraveineuse de CrCl₂ ou CrCl₃.

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This work was supported by the National Institute of Health, U.S. Public Health Service (Grants Nos. A-1641/C3, B-2037/C2, and H-6182) and by the Office of the Surgeon General, U.S. Army Medical and Research Command, Contract No. DA-193-MD-2039.     

The fiber spectrum of the cat VI nerve to the lateral rectus and retractor bulbi muscles

Zusammenfassung Das Faserspektrum des VI Hirnnerven der Katze wurde an 3 Stellen bestimmt: Austrittsstelle aus dem Pons, Verzweigungsstelle zum M. rectus lateralis und zum M. retractor bulbi. Wenigstens 3 verschiedene Typen motorischer Fasern wurden ermittelt: 1. grosse, dick myelinisierte, 2. kleine, dünn myelinisierte, 3. kleine, dick myelinisierte. Die Verzweigungsstelle zum M. retractor bulbi besteht hauptsächlich aus dem 1. Fasertyp.

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This investigation was supported by Public Health Service Program Project Research Grant No. NB 06038, General Research Support Grant No. 5 SO1 FR-05566, and Research Career Program Award No. K3-NB-14,094 to Dr.Bach-y-Rita from the National Institute of neurological Diseases and Blindness.     

Daily variations in pigeon cropsac responses to prolactin

Zusammenfassung Es wird festgestellt, dass die Ansprechbarkeit des Taubenkropfsackes auf Prolactin in Abhängigkeit vom hormonalen Zustand des Versuchstieres von Tag zu Tag verschieden ist.

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Our study was supported by a Public Health Service Research Grant No. 5 R01 AM13400 and a Public Health Service Research Career Development Award No. GM-17, 898, toAlbert H. Meier. The ovine prolactin was a gift of the Endocrinology Study Section of the National Institutes of Health. We thank Dr.P. E. Schilling of the Computer Center at Louisiana State University for making the statistical evaluations.     

Choline activation of lithium transport

Zusammenfassung Nachweis einer Erhöhung des Li-Austausches in Rinder-Erythrozyten bei Anwesenheit von Cholinchlorid im Extrazellularraum.

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This work was supported in part by a grant from the National Institutes of Health, a National Institutes of Mental Health Special Fellowship to W.R.C., and a Public Health Service Career Development Award to D.F.M.     

Transient ischemic alteration of synaptosomal neutral acid uptake

Summary The effect of cerebral ischemia and postischemia on the synaptosomal uptake of some neutral amino acids was determined in mongolian gerbils at various periods of time. A transiently increased uptake of³H isoleucine,¹⁴C cycloleucine and³H phenylalanine in the synaptosomes was found after 3 min of bilateral common carotid occlusion which returned to normal at 30 min of reestablished cerebral blood circulation.     

Effect of adrenalectomy on the response of rat liver polyribosomes to whole-body gamma radiation

Résumé L'adrénalectomie supprime le net déplacement, causé par l'irradiation, vers les espéces de polyribosomes plus lourdes observées chez les rats normaux irradiés. Ces résults font supposer que l'action de l'irradiation sur les polyribosomes du foie est réglée au moins en partie, par la glande surrénale.

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Recipient of U.S. Public Health Service Research Career Development Award No. CA 38655 from the National Cancer Institute and on special leave from August 1969–July 1970 at the Département de Radiobiologie, Centre d'Etude de l'Energie Nucléaire, Mol (Belgium).