Basic proteins of mouse ova and blastocysts

Zusammenfassung Mit einer speziellen Färbetechnik auf basische Proteine wird gezeigt, dass die Intensität und damit auch die Quantität der basischen Proteine beim übergang vom 4-Zell-Stadium zum 8-Zell-Stadium im Cytoplasma stark zunimmt. Daraus wird der Schluss gezogen, dass erst zu diesem Zeitpunkt die genetische Information für die Proteinsynthese wirksam wird.     

In vitro fertilization of mouse and hamster eggs after freezing and thawing

Summary Mouse eggs (12–14%) and hamster eggs (87–94%) appear normal upon thawing after having been stored for 30 min to 25 h at –70°C and –196°C. While 89–98% of the normal appearing hamster eggs are capable of fertilization in vitro, only 4–13% of the mouse eggs can be fertilized. The ability of fertilized eggs to develop in culture and their viability upon transfer to pseudopregnant recipients is under investigation.This work was supported by grants from the U.S. Public Health Service and the Ford Foundation. Sincere thanks are due to Prof.Y. Nishikawa of Kyoto University, Japan, for advice and to Mrs.Rose Bartke and Ms.D. M. Hunt for assistance.     

Effect of diethylstilbestrol on glucose absorption by rat small intestine in vitro

Resumen El dietilestilbestrol inhibe la absorción activa de glucosa en el intestino de rata in vitro, aunque no impide la entrada del azucar en las células de la mucosa. La difusión pasiva de arabinosa y xilosa es facilitada por la droga.     

Effect of mecloqualone on the oxygen and glucose uptake by brain tissue in vitro

Resumen La mecloqualona inhibe in vitro, la respiración de cortes y homogenizados de cerebro, incrementando el consumo de glucosa; este último efecto no se produce cuando al medio de incubación se añaden 100 mM de potasio.     

Effect of Ketamine hydrochloride on oxygen and glucose uptake by brain tissue in vitro

Resumen El clorhidrato de Ketamina (1×10^–3 M y 1×10^–4 M) incrementa el consumo de oxigeno de cortes de cerebro de rata incubados en Krebs-Ringer fosfato conteniendo 5 mM ó 100 mM de potasio. La droga no modifica el consumo de oxigeno de homogeneizado de cerebro total.     

Iproniazid (marsilid) reverses dopamine-effect and stimulates the growth of mouse neuroblastoma in vitro

Zusammenfassung Die hemmende Wirkung von Dopamin auf die Zellteilung von Neuroblastoma beruht wahrscheinlich nicht auf dem Effekt seines desaminierten Metaboliten, obwohl sie durch den MAO-Hemmer Marsilid aufgehoben wird.     

Effect of azimexon (BM 12.531) on mouse granulocyte-macrophage and monocyte-macrophage progenitor cells

Summary Treatment of mice with 25 mg/kg azimexon (BM 12.531) resulted in an increase in granulocyte-macrophage colony-forming cells (GM-CFC) in spleen and bone marrow after a transient depression in the cell populations. Bone marrow monocyte-macrophage colony-forming cells (MM-CFC) increased at 7 days after treatment, and splenic MM-CFC were least affected by azimexon treatment. The increase in granulocytic and monocytic colony-forming cells may play a role in the previously reported protection by azimexon against radiation and drug-induced toxicity.Supported by the Armed Forces Radiobiology Research Institute, Defense Nuclear Agency, under Research Work Unit MJ 00026.