新型磁性亲和载体的制备及其对溶菌酶的吸附

对兼具亲和作用和磁分离优点的磁性亲和吸附过程进行了研究。采用氯化沉淀法制备强磁响应性纳米级磁流体，并通过聚乙烯醇包埋，获得磁性高分子载体。经NaBH4还原和活性色素辛巴蓝修饰，使载体具有特异性亲和吸附能力。通过透射电镜、红外光谱、磁化率测定等手段对载体物性进行表征，以溶菌酶为目标蛋白，考察了吸附时间及温度对载体吸附性能的影响。     

分子印迹磁性荧光复合微球的制备及其性能研究

复杂生物体系中蛋白质的高效分离分析在生物分离、蛋白质纯化与检测等生命科学研究领域中具有重要的意义.本文以磁性荧光复合微球(Fe₃O₄MNP-ZnSQDs)为载体,利用表面印迹技术在Fe₃O₄MNP-ZnSQDs表面构建"核-壳"结构的磁性荧光蛋白印迹微球(Fe₃O₄MNP-ZnSQD@MIPs),并用于溶菌酶蛋白的快速分离.结果表明,制备的Fe₃O₄MNP-ZnSQD@MIPs具有分散性好、粒径均一、荧光发射强、磁响应明显等特点.在最优条件下,该印迹微球在15 min达到吸附平衡,最大吸附容量可达645.76 mg·g^-1,饱和磁强度为40 emu·g^-1,且具有良好的选择性,印迹因子为2.15.该磁性荧光分子印迹微球成本低、耗时短、使用简单、吸附量高且选择性好,可用于大批量样品检测中溶菌酶的快速分离与纯化.     

染料亲和膜的制备及其吸附白蛋白的性能研究

以尼龙66膜为基膜,采用甲醛活化法偶联壳聚糖制备尼龙-壳聚糖复合膜,再固载染料Cibacron Blue F3GA作为配位基,制备亲和膜.膜上壳聚糖和Cibacron Blue F3GA的含量分别为112.4和123.3 mg/g尼龙膜.通过静态和动态实验测定了牛血清白蛋白在膜上的吸附性能,结果表明:牛血清白蛋白在膜上的吸附平衡符合Langmuir方程;在蛋白溶液的pH值为4.7和较低的离子强度下对牛血清白蛋白具有较高的吸附量;在动态操作条件下容易达到吸附平衡.     

结合适配体技术的磁性粒子表面蛋白分子印迹材料研制

本文将具有高度特异性的生物分子核酸适配体引入分子印迹技术,并结合磁性纳米粒子材料比表面积大、操作简便性和易于修饰性于一体,制备出了新型蛋白分子印迹材料.首先获得性能更好的硅烷化的磁性微球,之后加入功能单体、交联剂和衍生化适配体,以溶菌酶为模板分子,制备出磁性溶菌酶蛋白分子印迹适配体-纳米粒子.实验结果显示,不同材料的蛋白吸附能力顺序由高到低分别为结合适配体的蛋白印迹材料,结合适配体的非蛋白印记材料,蛋白印迹材料和无适配体的非蛋白分子印记材料.初步研究表明,适配体与磁性粒子的结合可以有效提高蛋白质分子印迹材料的印迹效果.     

磁性分子印迹材料去除环境水体中的2,4-二硝基苯胺

为去除环境水体中的2,4-二硝基苯胺(DNAN),本研究结合分子印迹技术与磁分离技术,制备对DNAN有特异性识别作用、并易于分离再生的磁性分子印迹聚合物(MMIPs)复合材料.通过扫描电子显微镜(SEM)、动态吸附试验和静态吸附试验等手段分别对磁性材料的表面形貌、选择性及吸附特性进行表征.结果表明,用二乙烯基苯(DVB)为交联剂制备的磁性分子印迹材料(DVB-MMIPs)具有较高的吸附容量.通过对DVB-MMIPs进行Scatchard分析发现,该MMIPs存在高亲和位点和低亲和位点2种结合位点,高亲和位点的最大吸附量Qmax为6.98×10~(-6)mol·g~(-1),平衡解离常数KD为4.68×10~(-6)mol·L~(-1);低亲和位点的最大吸附量Qmax为5.71×10~(-4)mol·g~(-1),平衡解离常数KD为1.40×10~(-3)mol·L~(-1).该磁性MMIPs对模板分子具有选择吸附性能,能够成功和方便地去除环境水体中的DNAN污染物,回收率较高,并克服传统吸附材料选择性低、难以再生重复利用的弊端.     

表面改性对羟基磷灰石蛋白吸附的影响

采用原子转移自由基聚合（ATRP）在羟基磷灰石（HAP）纳米粒子表面接枝了聚甲基丙烯酸甲酯（PMMA）。随着接枝的PMMA含量增加,改性粒子在水溶液中的分散性增强。以牛血清白蛋白（BSA）和溶菌酶（LSZ）为吸附目标,研究了HAP和改性后的粒子（g-HAP）的蛋白质吸附性能。结果发现,g-HAP比HAP对蛋白质的单位吸...     

镍化磁性琼脂糖凝胶微球的制备及其在蛋白纯化中的应用研究

以自制磁性琼脂糖微球为基质,环氧氯丙烷为活化剂,亚氨基乙二酸作为螯合剂,制备了表面螯合Ni2＋的磁性凝胶微球（Mag—Agarose—Ni）．IR结果表明Ni2＋成功螯合到了磁性凝琼脂糖胶微球上;SEM结果显示在螯合Ni2＋后,Mag—Agarose-Ni形貌没有发生明显变化,且平均粒径为23btm;原子吸收光谱结果表明Mag—Agarose-Ni表面螫合的Ni2＋的量为2．12×10mol／mg;磁性能测试表明Mag—Agarose-Ni具有超顺磁性,其磁饱和强度为20．8emu／g,具有良好的磁响应性．将Mag—AgaroseNi用于六聚组氨酸融合蛋白K8的纯化研究,SDS-PAGE结果表明Mag—Agarose—Ni较市售Ni—NTA对K8具有更优的亲和分离效果,经15min的孵育后,Mag—Agarose—Ni对K8的吸附容量可达到8．8μg／mg．     

聚苯乙烯为致孔剂的分子印迹膜对溶菌酶吸附性能的研究

探索聚苯乙烯微球的添加对于分子印迹膜对溶菌酶(Lysozyme, Lyz)吸附性能的影响.以溶菌酶为模板分子,丙烯酰胺、N,N'-亚甲基双丙烯酰胺为功能单体、聚苯乙烯(Polystyrene,PS)微球为致孔剂,制备聚苯乙烯为致孔剂的分子印迹聚合物膜(PS-MIP).PS-MIP的吸附平衡时间为30 min,短于未添加聚苯乙烯微球的分子印迹聚合物膜(MIP);PS-MIP与MIP对于Lyz的吸附能力均明显好于聚苯乙烯为致孔剂的非分子印迹聚合物膜(PS-NIP)与未添加聚苯乙烯微球的非分子印迹聚合物膜(NIP);PS-MIP对Lyz的选择性要好于MIP,而卵清蛋白和牛血清白蛋白在PS-MIP和MIP上的荧光信号没有差别.实验结果表明,在分子印迹膜聚合过程中添加聚苯乙烯微球,有利于缩短吸附时间,增大溶菌酶的吸附量并提升选择性.     

径向亲和整体色谱柱的制备及性能

采用外部换热中心移热式反应器合成了环形整体固定相基质,对基质进行化学改性制备了弱阴离子(DEAE)整体色谱柱,分别采用健合及吸附法将牛血清白蛋白(BSA)固载到基质上,制备适用于快速分离当归有效成分的径向亲和整体柱,并考察了整体色谱柱的分离性能。结果表明吸附牛血清白蛋白(BSA)色谱柱的分离性能优于健合蛋白色谱柱。     

高盐条件下溶菌酶在疏水表面的折叠吸附

采用摇床法测定不同硫酸铵浓度时天然溶菌酶（Lys）在疏水作用色谱填料PEG-600上的吸附等温线,运用计量置换理论（SDT）计算出不同盐浓度条件下蛋白在吸附过程中的吸附自由能.得出在高盐浓度时（大于1.5mol/L）,总的置换吸附自由能为负值,推断出吸附是自发进行的,且可以定量计算出蛋白与PEG-600之间的吸附亲和能与水分子的解吸能.结合与吸附等温线相同条件下焓变测量数据表明,随盐浓度增大熵值增加,是因为去水合作用导致的熵增大为吸附提供了驱动力的缘故.其结果可以很好地解释液/固界面上折叠吸附的规律.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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Brief Introduction to “Journal of Jilin University( Science Edition) ”

<正>"Journal of Jilin University(Science Edition)"is a comprehensive academic journal in the fields of science sponsored by Jilin University and administrated by the Ministry of Education of the Peoples Republic of China.The journal started publication in 1955.The original name at starting publication was"Journal of Natural Science of Northeast People University",which was changed into"Acta Scientiarum Naturalium Universitatis Jilinensis"in 1958 owing to the name change of the university.The present journal name has