Mass spectrometric determination of the amino acid sequence in peptides: Desulfurization of sulfur-containing peptides

, N- 20 - .     

Mass spectrometric determination of the amino acid sequence in cystine- and cysteine-containing peptides

, - , , S-- S- .     

The linear amino acid sequence α1 acid glycoprotein

Zusammenfassung Es gelang, die lineare Aminosäuresequenz von saurem ₁-Glykoprotein (Orosomucoid), einem Globulin des menschlichen Plasmas, aufzuklären.     

Volumetric determination of amino acid and peptide hydrazides

Résumé Nous avons déterminé par titrage volumétrique une série d'hydrazides peptidiques et testé les titrages acides, basiques et oxidants. Un tableau condense les résultats obtenus avec les trois meilleurs réactifs de titrage (acide perchlorique dans l'acide acétique glacial, l'hypobromite de sodium et le permanganate de potassium) qui sont tous utilisables à cet effet.

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Acknowledgment. H. J.Goren is grateful for the Post-doctoral Fellowship awarded him by the National Research Council of Canada, which enabled him to carry out this research.     

The N-terminal amino acid sequence of the small subunit of ribulose-1,5-diphosphate carboxylase fromNicotiana tabacum

Summary The N-terminal sequence of the small subunit of Fraction I protein isolated from tobacco was investigated, using an automatic protein sequencer. The amino acid sequence of the first 21 residues is presented.     

Amino acid conjugates in blood and urine. I. A simple method for n-substituted amino acid determination

Zusammenfassung Eine neue, vereinfachte, elektrophoretische Trennungstechnik zur quantitativen Bestimmung von Aminosäuren in Körperflüssigkeiten in konjugierter Form oder als ninhydrin-negative Polypeptide wird beschrieben.     

The amino acid sequence of the agglutinin isolated from the red marine alga Bryothamnion triquetrum defines a novel lectin structure

The primary structure of a lectin isolated from the red alga Bryothamnion triquetrum was established by combination of Edman degradation of sets of overlapping peptides and mass spectrometry. It contains 91 amino acids and two disulphide bonds. The primary structure of the B. triquetrum lectin does not show amino acid sequence similarity with known plant and animal lectin structures. Hence, this protein may be the paradigm of a novel lectin family.     

Teratogenic effects of the amino acid leucine in the chicken

Zusammenfassung Leucin wurde in einer Dosis von 0,2 ml einer 1%igen Lösung im Amnion von 9 Tage bebrüteten Hühnereiern injiziert. Morphologische Anomalien mit abnormen Funktionen, meistens in den Beinen und der Nackenregion, wurden beobachtet, während Röntgen-Aufnahmen Stellungsanomalien in Gelenken der Extremitäten und der Halswirbel zeigten.

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This work was aided by grants from Sigrid Juselius Foundation.     

Expansion of amino acid homo-sequences in proteins: Insights into the role of amino acid homo-polymers and of the protein context in aggregation

Expansion of amino acid homo-sequences, such as polyglutamines or polyalanines, in proteins has been directly implicated in various degenerative diseases through a mechanism of protein misfolding and aggregation. However, it is still unclear how the nature of the expansion and the protein context influence the tendency of a protein to aggregate. Here, we have addressed these questions using spinocerebellar ataxia type-3 (ATX3) protein, the best characterised of the polyglutamine proteins, chosen as a model system. Using a transfected mammalian cell line, we demonstrate that ATX3 aggregation is noticeably reduced by deletion or replacement of regions other than the polyglutamine tract. The nature of the amino acid homo-sequences also has a strong influence on aggregation. From our studies, we draw general conclusions on the effect of the protein architecture and of the amino acid homo-sequence on pathology. Received 3 March 2006; received after revision 19 April 2006; accepted 22 May 2006