人免疫缺陷病毒2型(HIV-2)穿膜蛋白基因片段克隆表达

以人免疫缺陷病毒２（ Ｈ Ｉ Ｖ２） 的原病毒基因组为模板,通过套式 Ｐ Ｃ Ｒ 扩增得到了 Ｈ Ｉ Ｖ２ 的一段特异性膜抗原蛋白基因片段;随后将此片段克隆入诱导表达载体ｐ Ｇ Ｅ Ｘ５ Ｘ１ ,获得了重组表达质粒ｐ Ｇ Ｅ Ｘ３６ Ｅ Ｎ Ｖ． Ｉ Ｐ Ｔ Ｇ 对重组表达菌株诱导后, Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 结果显示重组菌株有特异性表达蛋白带产生     

电子辐照NTD FZ Si等温退火特性

本文报导了关于高阻Ｎ型区熔ＮＴＤ－Ｓｉ－Ｐ＾＋Ｎ结二极管经电子辐照后的等温退火特性，获得５个缺陷能级：Ｅ１＝０．１６ｅＶ，Ｅ２＝０。．２７ｅＶ，Ｅ３＝０．３１ｅＶ，Ｅ４＝０．３７ｅＶ６和Ｅ５＝０．４２ｅＶ，结果表明Ｅ３和Ｒ４有比其它３个能极更好的热稳定性。     

X线头影测量分析及计算机处理在正颌外科的应用

根据正颌外科的临床需要，采用自然头颅位拍摄Ｘ线头影测量片，以通过鼻点的真正垂直线为基准平面，充分反映颌面部畸形部位和有助于正颌手术设计的１６个角度及１０个距离为测量项目，对２０名广东籍面容端正、咬正常成年女性的Ｘ线头影测量片统计分析，获得了一系列可运用于临床使用的正常标准值，其中：ＳＮ／ＶＰ（８４．５±３．６）°、ＮＡ／ＶＴ（５．５±２．１）°ＮＢ／ＶＰ（５．３±１．１）、ＮＰ／ＶＰ（４．０±２．１）°、ＥＬ／ＶＰ（１８．８±３．８）°。并建立了一套新的具有颅颌面畸形分析、设计模似手术及术后面型预测等功能的正颌外科计算机处理系统。     

健康学龄前儿童鼻通气功能及临床意义

目的：为明确健康学龄前儿童鼻阻力情况和与之相关的鼻腔解剖学参数。方法：应用声反射鼻测量遥计对２８１名正常广东籍健康学龄前儿童的鼻腔进行测量。结果：学龄前儿童的鼻阻力（ＮＡＲ）有随着年龄的增加而逐渐减小的趋势,２、３岁年龄组儿童的鼻阻力明显大于５、６岁年龄组（Ｐ〈０．０５）;鼻腔容积（ＮＣＶ）和鼻腔最小横截面面积（ＮＭＣＡ）则随着年龄的增大而逐渐增大。２、３岁年龄组儿童ＮＣＶ和ＮＭＣＡ明显小于５、６     

红细胞生成素(EPO)在中国仓鼠卵巢细胞(CHO)中稳定表达

将ＥＰＯｃＤＮＡ分别插入真核表达质粒载体ｐＳＶ２ｄｈｆｒ和ｐｃＤＮＡ３的适当位点,用电脉冲法导入ＣＨＯ细胞,用ＥＬＩＳＡ法检测ＥＰＯ的表达量,用ＴＦ１细胞检测ＥＰＯ表达产物的生物活性．结果表明,用ｐＳＶ２ｄｈｆｒ作为载体,在抗ＭＴＸ阳性细胞克隆中,获得了表达ＥＰＯ（２５８ｎｇ／ｍＬ培养液）的ＣＨＯ细胞株,并且表达产物具有生物活性．进一步的传代和冻存试验表明,所得细胞株表达ＥＰＯ的水平不受传代和冻存的影响     

4,5-二溴邻硝基苯基荧光酮非离子表面活性剂分光光度法测定微量钼

研究了钼４，５二溴邻硝基荧光酮（ Ｂｒ Ｏ Ｎ Ｐ Ｅ）吐温４０ 显色体系的吸收光谱特性，在吐温４０ 存在下，钼（Ⅵ）与 Ｂｒ Ｏ Ｎ Ｐ Ｆ形成红色络合物．在 １．１～１．３ ｍ ｏｌ／ Ｌ 的盐酸介质中，络合物的吸收波长为 ５４０ ｍ ｍ ，摩尔吸光系数为 ９．５×１０４ Ｌ·ｍ ｏｌ·ｃｍ － １ ．钼（Ⅵ）的含量在 ０～１４ μｇ／２５ ｍ ｌ的范围内，符合比耳定律．多数常见阳离子不干扰显色反应．本法用于合金钢标样中的微量钼，获得了满意的结果．     

鼻咽癌细胞株,瘤株中EBV—LMP1基因的检测及变异性分析

采用ＰＣＲ法检测了不同代次的鼻咽癌细胞株、瘤株（ＳＵＮＥ／ＳＵＮＴ）中的ＥＢＶ－ＬＭＰ１基因片断，并分析其变异性。结果发现直到１３１代ＳＵＮＥ、３０代ＳＵＮＴ中仍然能检测到ＬＭＰ１基因片断，且在传代过程中ＬＭＰ１基因未发生变异。但与Ｂ９５－８中的ＥＢＶ－ＬＭＰ１基因相比，ＬＭＰ１基因发生了变异，表现为Ｘｈｏｌ、Ｍｓｐ酶切位点的消失和ＳＳＣＰ单链迁移率的不同。该结果提示变异的ＥＢＶ－ＬＭＰ１基因可能与ＳＵＮＥ／ＳＵＮＴ肿瘤的特性有一定的相关性。     

基于AxPe1280V芯片的多标准视频编码方案

介绍了一种基于ＡｘＰｅ１２８０Ｖ的Ｈ．２６１／Ｈ．２６３／ＭＰＥＧⅠ编码方案。ＡｘＰｅ１２８０Ｖ是一种可编程可配置的基于混合编码的视频处理芯片,可应用在可视电话、会议电视、ＪＰＥＧ、ＭＰＥＧⅠ、ＭＰＥＧⅡ、数字电视等众多领域。采用ＡｘＰｅ１２８０Ｖ对于多标准视频编解码只需通过软件控制来实现,无须改变硬件工作平台,给用户提供了极大的方便。     

鸽禽Ⅰ型副粘病毒油佐剂灭活苗免疫效果观察

用鸽Ａ／ＰＭＶⅠ油佐剂灭活苗和ＮＤＶＬａｓｏｔａ 油佐剂灭活苗分别免疫肉种鸽,２０ ｄ 抗体水平达到峰值,在３ ．００ｌｏｇ２ 以上维持３０ ｄ 以上。４０ ｄ 后用鸽Ａ／ＰＭＶⅠ和ＮＤＶＦ４８Ｅ９ 分别进行攻击,对于ＮＤＶＦ４８Ｅ９ 的攻击,保护率均为１００ ％ ,对于鸽Ａ／ＰＭＶⅠ的攻击,鸽Ａ／ＰＭＶⅠ油佐剂灭活苗保护率为１００ ％ ,ＮＤＶＬａｓｏｔａ 油佐剂灭活苗保护率为６６．７ ％ 。田间试验,抽检４８ 份血样,抗体平均为４ ．３０ｌｏｇ２     

N-芳酰基氨硫羰基甘氨酰甘氨酸衍生物的合成及其生物活性研究

在固液相转移催化条件下,以聚乙二醇６００（ＰＥＧ６００）为催化剂,通过芳酰氯、硫氰酸铵以及甘氨酰甘氨酸反应,以良好的产率合成了Ｎ芳酰基氨硫羰基甘氨酰甘氨酸．初步生物活性试验表明,Ｎ芳酰基氨硫羰基甘氨酰甘氨酸衍生物对小麦幼苗的生长具有显著的促进作用．     

An ERP study for the degrees of the difficulty in visual search

The present study mainly probed the relationships between ERP components and the degrees of difficulty in visual search. Three experimental and one controlled conditions were randomly selected. Results showed that the P1, N1, and P2 amplitudes and latencies had no significant differences among the three experimental and one controlled conditions, and the ERP waveforms of low perceptual load of visual search were different from that of high perceptual load. These results might indicate that the latencies and amplitudes of initial P1, N1 and P2 in high and low perceptual load conditions were almost the same. The ERP waveforms of parallel search were very different from that of serial search, and the ERP waveforms of serial search were very similar. The P3 amplitudes increased as the degrees of the difficulty in visual search decreased.     

An ERP study for the degrees of the difficulty in visual search

The present study mainly probed the relationships between ERP components and the degrees of difficulty in visual search. Three experimental and one controlled conditions were randomly selected. Results showed that the P1, N1, and P2 amplitudes and latencies had no significant differences among the three experimental and one controlled conditions, and the ERP waveforms of low perceptual load of visual search were different from that of high perceptual load. These results might indicate that the latencies and amplitudes of initial P1, N1 and P2 in high and low perceptual load conditions were almost the same. The ERP waveforms of parallel search were very different from that of serial search, and the ERP waveforms of serial search were very similar. The P3 amplitudes increased as the degrees of the difficulty in visual search decreased.     

A ballistocardiogram measurement system for home monitoring: design,performance, and evaluation

We present a ballistocardiogram （BCG） measurement system for obtaining the BCG signal. The performance of the BCG measurement system was tested, including the dynamic response frequency of the platform （32 Hz with a 75 kg load）, and the signal to noise ratio of the system （minimum 23 dB）. Factors that could influence BCG recording during measurement were investigated by collecting data from 48 healthy subjects （28 males and 20 females）. The basic features of the BCG signal, such as the amplitude and time interval, were extracted, and differences by gender were analyzed. A correlation between the IJ amplitude （amplitude difference between I-and J-peak） and the respiration phase was found （R2 = 0.49）. The BCG signals for both sitting （SiBCG） and standing （StBCG） postures were obtained using this system. These showed that the StBCG amplitudes were larger than those of SiB- CG but there was no significant difference in the time intervals. The robustness of our system was verified, and its potential for noninvasive cardiac activity monitoring was demonstrated.     

野木瓜注射液对牛蛙坐骨神经传导的影响

10％野木瓜注射液（InjectionStauntoniae，IS）涂布于离体牛蛙坐骨神经8min后，神经干复合动作电位诸成分的潜伏期都发生了显著性的延长，但只对C2成分的幅度有显著性影响；而50％IS不仅使复合动作电位各类成分的潜伏期都发生了显著性延长，而且使除Aαβ和C1以外的各类成分的幅度有显著性的减小．上述IS的影响具有明显的量效关系．这种阻滞作用是可逆的．     

Testes and chromosomes in interspecific hybrids betweenHelicoverpa armigera (Hübner) andHelicoverpa assulta (Guenée)

The interspecific hybridization betweenHelicoverpa armigera females andHelicoverpa assulta males yieldedF ₁ hybrids (RS), fertile males and sterile individuals with abnormal genitals. The reverse hybridization betweenH. assulta females andH. armigera males yielded F₁ hybrids (SR)-fertile males and fertile females. The morphology of testes and the karyotype of chromosomes of larvae in the hybrids were investigated. Among the 2d old fifth-instar SR larvae, individuals without testes were fertile females and those with testes were fertile males. The length and breadth of testes between SR and parental species were not significantly different (p>0.05). Among the 2d old fifth-instar RS larvae, the testes were observed in all the individuals, but it could be classified into two types. The length and the breadth of testes in Type 1 larvae were not significantly different from those of their parental species (p>0.05), while those in Type 2 were significantly less than those of their parental species (p<0.01). Mitotic metaphase I of brain cells showed the diploid chromosomes number of both reciprocal hybrids was 2n=62, as many as their parents. The haploid number of 31 was confirmed by counts from spermatocytes at meiotic metaphase from SR male larvae and Type 1 larvae of RS. Meiosis was not observed in spermatocytes of Type 2 larvae of RS. Considering the characteristics of adult hybrids of RS, it was concluded that Type 1 individuals in RS were fertile and those of Type 2 were sterile. The sterility of Type 2 individuals in RS is attributed to the abnormity in development of testes and the failing meiosis of spermatocytes. As a result, the normal spermatozoon could not been produced.     

Neural correlates of short-term perceptual learning in orientation discrimination indexed by event-related potentials

The current work investigated the neural correlates of visual perceptual learning in grating orientation discrimination by recording event-related potentials (ERPs) from human adults. Subjects were trained with a discrimination task of grating orientation in three consecutive training sessions within 2 h. While reaction times (RTs) were shortened gradually across training sessions, the N1 was decreased and the P2 was increased over the parietal and occipital areas. A broadly distributed P3 was increased along with more practices. In addition, the time course of learning reflected in the P2 and P3 amplitudes was in line with the changes of reaction times and exhibited a stable level during later training. The impli- cations of these results to the neural mechanisms subserving perceptual learning were discussed.     

临夏回族青少年体质发育特征研究

本文对甘肃省临夏回族13—17岁青少年500人(男246,女254)的34项体质指标进行了活体测量,计算出其平均值、标准差和变化范围,进行了男女性别的比较。结果表明年平均增长率以体重为最高;头长、头宽、头冠状弧、额最小宽、两眼外角宽、容貌耳宽、肩宽、骨盆宽和足宽的年平均增长率女性大于男性;唇高、形态面长、手长、口裂宽男女相近;而其余21项指标的年平均增长率男性大于女性,其中身高、坐高、髂前棘点高、胫骨点高在男女之间有显著的差异(p<0.05)。     

Early ERP effects on the scaling of spatial attention in visual search

This article describes the examination of the spatial‘scaling＇ effect of visual attention with the technique of event-related potential （ERP）. Eighteen participants were involved in a visual search task in which the cue-target paradigm was used. The search array was three concentric circles consisting of randomly selected English letters that were equally distributed in each circle. The behavioral and ERP data were recorded, respectively. The behavioral results showed that the response time increased and the response accuracy decreased with the increase of precue size. The ERPs amplitude of PI and NI components evoked by search array increased with the reduction of precue size. However, the latencies of these ERP components did not show significant differences between conditions. The hierarchical data of both behavioral assessment and ERPs provided evidence for the spatial ＇scaling＇ effect of visual attention. The amplitudes of PI and N 1 components may be used as indices to examine the effect of spatial ＇scaling＇. In different tasks, the display-set size of stimuli and the task complexity may be important factors that affect the attention allocation.     

甲型H1N1流感患儿免疫球蛋白水平变化

目的探讨甲型H1N1流感患儿免疫球蛋白水平变化及临床意义。方法采用放射免疫法检测51例甲型H1N1流感患儿（其中非危重组31例,危重组20例）急性期和恢复期以及对照组25例健康儿童外周静脉血的Ig,A、IgG、IgM、C3水平,并对结果进行分析。结果非危重组急性期和恢复期及对照组血清IgA、IgG、C3水平两两比较,差异均无统计学意义（P〉0．05）;危重组急性期血清IgA、IgG、C3水平低于非危重组急性期和对照组（P〈0．05）,恢复期血清IgA、I如、C3水平上升,与急性期比较差异有统计学意义（P〈0．05）;危重组和非危重组恢复期血清IsA、Is,G、C3水平比较,差异无统计学意义（P〉0．05）。非危重组、危重组及对照组血清IgM两两比较,差异无统计学意义（P〉0．05）。结论甲型H1N1流感患儿免疫球蛋白水平变化与病情严重性有关,危重患儿表现为急性期血清IgA、IgG、C3水平降低,恢复期恢复正常,提示危重甲型H1N1患儿体液免疫抑制是暂时的、可逆的。     

怒族成人Heath-Carter法体型研究

运用Heath-Carter人体测量体型方法对云南省怒江州的贡山县丙中洛乡和福贡县匹河乡怒族成年人317倒（男为183例，女为134倒）进行了体型评定．结果表明：（1）怒族成人平均体型男性为均衡的中胚层体型（2．1—4．9—2．6），女性为内胚层-中胚层均衡体型（4．3—4．5-1.7）．（2）随着年龄增长，怒族男女在20-岁组到40-岁组体型均呈内因子值增大、外因子值减小的趋势；50岁以后内因子值减小，外因子值增大；中因子值，男性在50岁前增大，50岁后减小，女性则一直增大．（3）男女问体型存在极显著性差异，女性中因子值、外因子值低于男性，而内因子值高于男性．（4）与其他群体相比，怒族内因子值偏低，中因子值偏高，外因子值偏高或居中，与侗族体型最接近（男性SAD=0．46；女性SAD=0．36），与布依族和仡佬族较为接近．