The mobilization of rat’s mesenchymal stem cells into peripheral blood by LiCL and its potency differentiation

Mesenchymal stem cells （MSCs） are multipotent cells, which can differentiate into different tissues. It is still controversial whether MSCs can be isolated from adult peripheral blood （PB） under normal conditions and whether they can be mobilized in a way similar to that of hematopoieUc stem cells （HSCs）. In this study, rat MSCs circulating in the PB under normal conditions can be isolated and cultured, and MSCs from PB and BM can be mobilized by LiCL. The mobilized MSCs can be induced to differentiate into neuron by such factors as β-mercaptoethanol and supernatants of nerve cell cultures. The present study provides a broader perspective on the repair of neural trauma.     

干细胞:让你“长生不老”?

您听说过息、壤的上古神话吗?传说尧时代,大禹的父亲鲧受命治理天下的洪水,但效果不甚理想。有一天他从天帝那里偷了一件宝贝以湮灭洪水,结果天帝派祝融去杀鲧。死之前,鲧把腹部剖开,生出了大禹。于是,大禹就拿着这件宝贝继续治水。这个宝贝就是息壤,一种可以自己生长、永不耗减的土壤。其实在我们每个人的体内,也有类似于息壤的东西——干细胞。它的存在和发现,有望让人类长生不老的终极梦想靠近现实。当然,万物有生必有死,绝对的长生不老是不可能实现的,但以现代医学     

β_2-Adrenoceptor affinity chromatography and its application in the screening of the active compounds from Semen Armeniacae Amarum

β2-Adrenoceptor (β2-AR) was purified from the rabbit lung tissue by sepharose-salbutamol affinity chromatographic column. To prepare the β2-AR stationary phase, β2-AR was evenly immobilized on the surface of macro-pore silica with a mild chemical coupling method through covalent bond. The reten- tion properties of β2-AR stationary phase were characterized by four ligands, salbutamol sulfate, noradrenaline bitartrate, adrenaline hydrochloride and propranolol hydrochloride, to establish the β2-AR affinity chromatography. Then, the method was used to screen the active compounds from the total extracts of Semen Armeniacae Amarum. The results showed that β2-AR on the surface of the sta- tionary phase could keep its original bioactivity and selectivity. Amygdalin retained in the chroma- tographic column was proved to be the active compound of the total extracts of Semen Armeniacae Amarum. Compared with the existing chromatographic screening approaches, this method showed a good stability and high selectivity. The active compounds which could interact with β2-AR in traditional Chinese medicine (TCM) could be screened efficiently by this method, providing a new way to screen the active compounds in complicated samples such as TCM.     

Optimization and application of blasting parameters based on the “pushing-wall” mechanism

The large structure parameter of a sublevel caving method was used in Beiminghe iron mine. The ores were generally lower than the medium hardness and easy to be drilled and blasted. However, the questions of boulder yield, \"pushing-wall\" accident rate, and brow damage rate were not effectively controlled in practical blasting. The model test of a similar material shows that the charge concentration of bottom blastholes in the sector is too high; the pushing wall is the fundamental reason for the poor blasting effect. One of the main methods to adjust the explosive distribution is to increase the length of charged blastholes. Therefore, the field tests with respect to increasing the length of uncharged blastholes were made in 12# stope of -95 subsection and 6# stope of Beiminghe iron mine. This paper took the test result of 12# stope as an example to analyze the impact of charge structure on blasting effect and design an appropriate blasting parameter that is to similar to No.12 stope.     

β2-Adrenoceptor affinity chromatography and its application in the screening of the active compounds from Semen Armeniacae Amarum

β2-Adrenoceptor （β2-AR） was purified from the rabbit lung tissue by sepherose-salbutamol affinity chromatographic column. To prepare the β2-AR stationery phase, β2-AR was evenly immobilized on the surface of macro-pore silica with e mild chemical coupling method through covalent bond. The retention properties of β2-AR stationery phase were characterized by four ligends, selbutamol sulfate, noredreneline bitartrete, adrenaline hydrochloride end proprenolol hydrochloride, to establish the β2-AR affinity chromatography. Then, the method was used to screen the active compounds from the total extracts of Semen Armeniacae Amarum. The results showed that β2-AR on the surface of the stationary phase could keep its original bioectivity end selectivity. Amygdalin retained in the chromatographic column was proved to be the active compound of the total extracts of Semen Armeniacae Amarum. Compared with the existing chromatographic screening approaches, this method showed e good stability end high selectivity. The active compounds which could interact with β2-AR in traditional Chinese medicine （TCM） could be screened efficiently by this method, providing e new way to screen the active compounds in complicated samples such as TCM.     

实现“生命再造”的梦想——解读2012诺贝尔生理学或医学奖

未来有一天,每个人都将成为自身健康的＂庇佑者＂,不论是衰老、创伤或者疾病,造成组织的缺损和伤害,都可以取自身的体细胞作为＂种子＂,让其重新＂逆转＂成多能干细胞,并进一步分化为心脏、神经、胰岛、肝脏、肾脏等多种类型细胞,甚至组织器官,最终实现＂生命再造＂的梦想。如今,在迈向这一梦想的阶梯上,一个被称为＂细胞核重编程＂的医学理论成为新的基石和里程碑。     

Concentrations of “elemental carbon” in samples from the Peking Man Site at Zhoukoudian and the possibility of their application in the development of evidence for the use of fire by humans

Elemental carbon?EC) is a C-rich, O-H-S-N-depleted substance that is necessarily produced in the process of combustion. Due to the logn-term use of fire by cave-inhabiting ape-men, considerable amounts of 揺lemen-tal carbon?would be necessarily left behind in the corre-sponding strata inside the cave. The sepaation of EC was effected by the chemical method and the contents of carbon were determined on an element analyser and a self-made measuring system. The concentration of Ec in the hearth,the exhibited ash samples collected from near the hearth un-earthed in the 1930s, and the three samples of accumulated material collected from the tenth bed are 43.74%, 1.77%,3.88%,1.87% and 1.11%,respectively; those of the equivalent samples from the tenth bed outside the cave and samples from the fourth and seventh beds are at least one order of magnitude lower than the former抯. The above re-sults show that the sampling location of the tenth bed may be close to the hearth. Sampling over a wider range may lead to the finding of a location where the concentrations of EC are corresponding to the maximum values of EC in the hearth. The determination and study of EC may provide new evi-dence for the use of fire by Peking Man.     

“Climate effect” of the northeast cold vortex and its influences on Meiyu

The Northeast Cold Vortex (NECV) is an important weather system in the middle and high latitudes in East Asia. Its time scale is synoptic, yet the frequent activities of NECV have significant \"climate effect\" which influences not only the monthly temperature in the lower troposphere in Northeast China but also the Meiyu rainfall in East Asia. On the basis of ERA-40 reanalysis data provided by ECMWF, the \"climate effect\" of NECV and its relationship with Meiyu in East Asia are studied. It is shown that there is significant correlation between NECV during the Meiyu period and rainfall amount: strong NECV corresponds to more Meiyu rainfall and weak NECV corresponds to less rainfall. In strong NECV years, the dry and cold air from the north is led to the south by NECV, converges with the lower-level warm and wet southwesterly on the north verge of Meiyu region, thus forms an unstable stratification of \"upper dryness and lower wetness\" . Triggered by ascending motion, the Meiyu rainfall amount is more than usual. It is on the contrary in weak NECV years. The anomalous SST in north Pacific in the previ-ous year may be a factor that results in the anomalous NECV at Meiyu period. The land-sea thermal contrast in summer facilitates NECV, while that in winter inhibits NECV. All of the above provide a meaningful result for the short-term climate prediction of NECV and Meiyu.     

The Similarities of Heart in English and “心” in Chinese in the use of Metaphor

Metaphor is a figure of speech which is often used both in written and spoken forms. There are both similarities and differences in the metaphorical uses of "heart" in English and the uses of its correspondent "心" in Chinese. The same metaphorical uses of "heart" and "心" are discussed in this paper.     

Phenomenon and mechanism of “secondary damage” during repair of DNA damage in irradiated mammalian cells

By means of comet assay, a study of kinetics curve of DNA damage repair in irradiated SX-9 cells that came from mouse breast cancer proceeded. It was found that while the initial DNA damages had nearly been repaired, DNA damages arose for the second time, then they were repaired again. As a result, a phenomenon of “secondary damage” was found during the repair of DNA damages in irradiated SX-9 cells. Further research illuminated that 3-aminobenzamide (3AB), which is an inhibitor of poly (ADP-ribose) polymerase, could change the proceeding of “secondary damage”. For this reason it is poasible that there exists some inner relationship between the phenomenon of “secondary damage” and the function of poly(ADP-ribose) polymerase.     

Comment on “Low-frequency sea level variation and its correlation with climate events in the Pacific”

The tide gauge results exhibit strong multi-decadal oscillations with detected periodicities up to a quasi-60 years. This translates in the requirement of at least 60-70 years of continuously recorded data without any quality issue and the use of a procedure appropriate for time series with periodic components to infer the acceleration trend. This is not what is done in many recent papers where the oscillating behaviour is neglected and short or incomplete records arbitrarily extended or reconstructed are used to infer wrong accelerating trends. All the tide gauges of enough length and quality show the sea levels are oscillating with important multi-decadal periodicities but absolutely not positively accelerating. The satellite radar altimeter reconstruction of the global mean sea level only available since 1993 shows despite the many uncertainties of a still imperfect procedure a not positively accelerating behaviour. The presence of acceleration in the reconstructed or extended data sets and the lack of acceleration in all the individual tide gauge measurements of enough quality and length certainly deserve further discussion. This is a comment to a previously published original paper made on the basis of other previously published results and not a new original paper.     

Zircon SHRIMP U-Pb ages of the “Xinghuadukou Group” in Hanjiayuanzi and Xinlin areas and the “Zhalantun Group” in Inner Mongolia, Da Hinggan Mountains

A report is presented of SHRIMP zircon U-Pb dating data of meta-igneous and meta-sedimentary rocks of the Xinghuadukou Group(Xinlin-Hanjiayuanzi area,Heilongjiang Province)and meta-volcanic rocks of the Zhalantun Group(Zhalantun district,Inner Mongolia).The SHRIMP analyses show that the meta-igneous rocks from the Xinghuadukou Group formed at 506±10―547±46 Ma,belonging to Early-Middle Precambrian,whereas the meta-sedimentary rocks yielded detrital zircons,with ages of 1.0―1.2,1.6―1.8 and 2.5―2.6 Ga,indicative of deposition age at least<1.0 Ga. Meta-basic volcanic rocks from the Zhalantun Group have a formation age of 506±3 Ma.These data suggest that both the Xinghuadukou and Zhalantun Groups formed during Cambrian and/or Neoproterozoic time,rather than Paleoproterozoic time as previously thought.Early Precambrian inherited zircons in the meta-igneous rocks and numerous Precambrian detrital zircons in the meta-sedimentary rocks imply that these rocks were formed proximal to older crust.It is inferred that the Xinghuadukou and Zhalantun Groups represent Cambrian and/or Neoproterozoic vol- cano-sedimentary sequences formed in an active continental margin setting.     

The expression and function of VEGF at embryo implantation “window” in the mouse

Vascular endothelial growth factor (VEGF) is an endothelial cell-specific mitogen that plays a critical role in angiogenesis. Recent reports indicated that VEGF was closely involved in embryo implantation and embryonic vasculogenesis. However, very little information is available about the detailed expression and function of VEGF at implantation “window”. In this work, VEGFs were primarily present on uterine epithelial cell monolayer and blastocysts including the outgrew trophoblasts at implantation window. VEGF antibodies decreased the number of mice embryos implanted and the percentage of blastocysts with attachment and outgrowth in a co-culture model in a dose-dependant manner. These findings demonstrate that VEGF is one of the essential cytokines for embryo implanta-tion in mouse. VEGF may act as a local mediator to regulate the maternal-fetal interaction, and facilitate blastocyst implantation.     

A comparison Between Mary and Warren in “The Death of the Hired Man”

《雇工之死》是一首无韵体叙事诗。强调了同情心和人际关系中相互理解的重要。该诗讲的是一对农家夫妇沃伦和玛丽对待雇工塞拉斯的态度,反映了夫妻二人的性格差异;沃伦是个讲求实效的农夫,起初他反对妻子再一次将塞拉斯收留,因为塞拉斯在农忙季节在别人的高价诱惑下离他们而去;玛丽心地善良,尤其善解人意。富有同情心,体谅到塞拉斯的难处。塞拉斯在故事中始终没有露面,他的性格和境遇完全由夫妻二人的对话表现出来。     

The Descriptive Study of “Sun Zi：The Art of War- Making Assessments”——Domestication and Foreignization in the English translation of the Article

The ideas of “Domestication” and “Foreignization” in Translation was put forward by the American scholar L. Venuti in 1995, indicating that there are two aspects involved in the process of translation. Of one is that a translation should get the readers closer to the author, while the other one is to get the author closer to the readers. The English translation of “Sun Zi： The Art of War” done by Lin Maosun is an outstanding example representing these two basic concepts in translation principles. It abounds with plenty of excellent examples demonstrating how domestication and foreignization can be achieved in the translation of Chinese classics.     

Reply to: Parker A. Comment on “Low-frequency sea level variation and its correlation with climate events in the Pacific”

First, we would like to thank Parker for his discussion and comments on our work. Parker’s main concern [1] is that the sea level is oscillating with important multi-decadal periodicities but absolutely not positively accelerating. He also argued that the global mean sea level (GMSL) acceleration computed by Jin et al. [2] seemed too large. His similar     

从“English and the pace of endangerment in Nigeria”引申并浅析英语全球化的影响

随着英语在全世界的传播,英语已逐渐成为了一种国际通用语(lingua franca);特别是其在周边国家及延伸国家的渗透,更会带来一系列的影响。本文从English and the pace of endangerment in Nigeria一文所体现出的英语全球化的不利影响入手,着重讨论了英语全球化的消极和积极的影响。     

Fast acceleration of “killer” electrons and energetic ions by interplanetary shock stimulated ULF waves in the inner magnetosphere

Energetic electrons and ions in the Van Allen radiation belt are the number one space weather threat. Understanding how these energetic particles are accelerated within the Van Allen radiation belt is one of the major challenges in space physics. This paper reviews the recent progress on the fast acceleration of "killer" electrons and energetic ions by ultralow frequency (ULF) waves stimulated by the interplanetary shock in the inner magnetosphere. Very low frequency (VLF) wave-particle interaction is considered to be one of the primary electron acceleration mechanisms because electron cyclotron resonances can easily occur in the VLF frequency range. Recently, using four Cluster spacecraft observations, we have found that, after interplanetary shocks impact the Earth’s magnetosphere, energetic electrons in the radiation belt are accelerated almost immediately and continue to accelerate for a few hours. The time scale (a few days) for traditional acceleration mechanisms, based on VLF wave-particle interactions to accelerate electrons to relativistic energies, is too long to explain our observations. Furthermore, we have found that interplanetary shocks or solar wind pressure pulses, with even small dynamic pressure changes, can play a non-negligible role in radiation belt dynamics. Interplanetary shocks interaction with the Earth’s magnetosphere manifests many fundamental space physics phenomena including energetic particle acceleration. The mechanism of fast acceleration of energetic electrons in the radiation belt responding to interplanetary shock impacts consists of three contributing parts: (1) the initial adiabatic acceleration due to strong shock-related magnetic field compression; (2) followed by the drift-resonant acceleration with poloidal ULF waves excited at different L-shells; and (3) particle acceleration due to the quickly damping electric fields associated with ULF waves. Particles end up with a net acceleration because they gain more energy in the first half of this cycle than they lose in the second. The results reported in this paper cast a new light on understanding the acceleration of energetic particles in the Earth’s Van Allen radiation belt. The results of this study can likewise be applied to interplanetary shock interaction with other planets such as Mercury, Jupiter, Saturn, Uranus and Neptune, and other astrophysical objects with magnetic fields.