The metabolism of 3-hydroxytyramine-1-C14 in brain tissue homogenates

Zusammenfassung Homogenate aus Gehirn wurden mit 3-Hydroxytyramine-1-C¹⁴ inkubiert. Als Stoffwechselprodukt wurde aus der sauren Fraktion radioaktive 3,4-Dioxyphenylessigsäure isoliert. Aus der basischen Fraktion wurden drei radioaktive Zonen papierchromatographisch isoliert. Die erste Zone konnte noch nicht identifiziert werden. Die zweite wurde als 3-Hydroxytyramin und die dritte als 3-Methoxytyramin erkannt. Die mögliche Rolle der Stoffwechselprodukte von 3-Hydroxytyramin beim Parkinson-Syndrom wird diskutiert.

---

---

This study was supported in part by United States Public Health Service Grant M 2717.The authors are grateful toR. J. Floody, M. D. of Hoffmann-La Roche Inc., for providing us with iproniazid.     

Direct metabolism of 7α-3H-DHEA-3-14C-glucuronoside

Zusammenfassung Es wird gezeigt, dass beim Menschen Steroide in Form ihrer 3-Glucuronoside metabolisiert werden können, und zwar in ähnlicher Weise wie dies schon für entsprechende 17-Glycoside nachgewiesen worden ist.     

Methionine metabolism in apple tissue

Summary A metabolic intermediate isolated from apple tissue fed either methionine or 5-methylthioadenosine has been tentatively identified as a methionine-pyridoxal Schiff base. The formation of this compound is discussed in relation to ethylene biosynthesis.Acknowledgments. This investigation was supported by Research Grant AO-150 from the National Research Council of Canada.     

Studies on DNA methylase activity in mammalian tissue

Resumen Para aislar nucleo de mamíferos que contienen DNA metilasa actividad se utilizó como medio homogenizante 0.25M sucrosa-1×10^–2 M EDTA. Hígado de rata y el hepatoma de Reuber demostraron cantidad equivalente de actividad enzimática. Se observó aproximadamente 100% de inhibición con amoníaco 0.15M, los iones de Mg no mostraron efecto.     

Potentials in mammalian skeletal muscle from collagenase-treated tissue

Summary Transmembrane potentials were recorded from skeletal muscle fibres dissected with the aid of collagenase perfusion. Collagenase treatment had little or no effect on the action or resting potentials.     

Direct metabolism of 7α-3H-dehydroepiandrosteron-35S-sulphate in human ovarian tissue

Zusammenfassung Nach Inkubation von menschlichem Ovarialgewebe mit 7-³H-DHEA-³⁵S-sulfat konnten als Metaboliten 5 verschiedene C₁₉- und 2 phenolische C₁₈-Steroide isoliert und identifiziert werden, die als Sulfokonjugate einen gegenüber dem Substrat unveränderten³H/³⁵S-Quotienten aufwiesen.

---

---

These investigations were supported by the Deutsche Forschungsgemeinschaft, Bad Godesberg (Germany).     

The metabolism of methadone by cultured mammalian cells

Summary Rat hepatoma tissue culture cells and mouse leukemic cells were found to metabolize [1-³H] methadone to at least 2 unidentified radioactive compounds. These results suggest that cultured cells may be useful models for studying methadone metabolism by specific cell types.This study was supported by United States Public Health Service Research Grant No. MH 15622. The illustrations were prepared by Diane J. Kastelic.     

Lipid characterization and 14C-acetate metabolism in catfish taste epithelium

The catfish, Ictalurus punctatus is an important model system for the study of the biochemical mechanisms of taste reception. A detailed lipid analysis of epithelial tissue from the taste organ (barbel) of the catfish has been performed. Polar lipids account for 62 +/- 1% of the total, neutrals for 38 +/- 1%. Phosphatidyl-cholines, serines and ethanolamines are the major constituents of the polar fraction. Plasmalogen concentration is high relative to that of non-neural tissues. [14C]-Acetate is incorporated into cell lipid fractions after incubation of barbel tissue at 37 degrees C for 60 min. Percentage amounts of most lipids change with time during this in vitro incubation. The phospholipids are the most metabolically active fractions. This work yields information for continuing reconstitution experiments and indicates that the taste epithelium of this important model system is a metabolically active tissue capable of supporting lipid turnover/synthesis.     

Lipid characterization and14C-acetate metabolism in catfish taste epithelium

Summary The catfish,Ictalurus punctatus is an important model system for the study of the biochemical mechanisms of taste reception. A detailed lipid analysis of epithelial tissue from the taste organ (barbel) of the catfish has been performed. Polar lipids account for 62±1% of the total, neutrals for 38±1%. Phosphatidyl-cholines, serines and ethanolamines are the major constitutents of the polar fraction. Plasmalogen concentration is high relative to that of non-neural tissues. [¹⁴C]-Acetate is incorporated into cell lipid fractions after incubation of barbel tissue at 37°C for 60 min. Percentage amounts of most lipids change with time during this in vitro incubation. The phospholipids are the most metabolically active fractions. This work yields information for continuing reconstitution experiments and indicates that the taste epithelium of this important model system is a metabolically active tissue capable of supporting lipid turnover/synthesis.This work was supported in part by NIH Research Grant No. NS-23622, NS-22620, and by the Veterans Administration.     

Potentials in mammalian skeletal muscle from collagenase-treated tissue.

Transmembrane potentials were recorded from skeletal muscle fibres dissected with the aid of collagenase perfusion. Collagenase treatment had little or no effect on the action or resting potentials.     

A simple technique for the measurement of 3H- and 14C-radioactivity per microgram DNA in fixed tissue

A simple, rapid and versatile technique for scintillation counting of double labelled fixed tissue is described. Furthermore, DNA determination can be performed on the same tissue digest.