共查询到20条相似文献,搜索用时 125 毫秒
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Drosophila Krüppel protein is a transcriptional repressor 总被引:45,自引:0,他引:45
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将编码人c-myc羧基端bHLH/LZ结构域的92个氨基酸的cDNA片段(c-myc-c92)对框插入pGEX-2T原核表达载体中,使之与谷胱甘肽转移酶(GST)编码基因融合,并将重质粒导入大肠杆菌BL21(DE3)中,由IPTG诱导融合基因高效表达,并用Glutathione Sepharose4B亲和柱纯化融合蛋白GST-c-Myc-c92,凝胶阻滞(EMSA)分析显示该纯化蛋白能与CACGTG序列特异结合,并且只有高浓度的GST-c-Myc-c92才能与探针结合,结果表明在体上情况下高浓度c-Myc羟基端能自身二聚体化,此发现丰富了Myc-Max-Mad的调控网络,并为进一步研究c-myc的功能和调控提供了一定的线索。 相似文献
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Involvement of the 'leucine zipper' region in the oligomerization and transforming activity of human c-myc protein 总被引:47,自引:0,他引:47
c-Myc plays a part in the regulation of important cellular processes such as growth, differentiation and neoplastic transformation. Although c-myc gene structure and expression are well characterized, the function and biochemical properties of the protein are less well understood. Human c-myc is a 439-amino acid phosphoprotein which binds DNA in vitro and belongs to a discrete subset of nuclear proteins. Using the human c-myc mutants generated by linker-insertion and deletion mutagenesis, we have defined regions of the protein that are important for its transforming activities and its nuclear localization. Here, we show that human c-myc exists as an oligomer in vitro and use mutant proteins to localize the oligomerization domain to a carboxyl-terminal peptide containing the 'leucine zipper' motif. The 'leucine zipper' describes a structure found in a number of DNA-binding proteins that contains leucines occurring at intervals of every seventh amino acid in a region predicted to be alpha-helical. The 'leucine zipper' might mediate dimerization by intermolecular interdigitation of the leucine side-chains. We show that a c-myc mutant, which is inactive but can oligomerize, dominantly inhibits the cotransforming activity with wild-type c-myc of rat embryo cells, whereas inactive mutants which cannot oligomerize properly because of deletions in the oligomerization domain are recessive. 相似文献
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New type of POU domain in germ line-specific protein Oct-4. 总被引:72,自引:0,他引:72
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IntroductionSevereenvironmentalchanges ,suchaslow temperature ,droughtandhigh salt ,affectthegrowthanddevelopmentof plantsandtheproductivityofcrops .Plantcellcannotobtainwaterwhensubjectedtodroughtorhigh saltcondition .Low temperaturealsoreduceswaterstateof … 相似文献
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Shi Y Sawada J Sui G Affar el B Whetstine JR Lan F Ogawa H Luke MP Nakatani Y Shi Y 《Nature》2003,422(6933):735-738
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