羊栖菜多糖对荷瘤小鼠红细胞膜Na+,K+-ATPase活性的影响

报道了羊栖菜多糖 （SFPS）对S180A小鼠,EAC小鼠,L615 小鼠红细胞膜Na+,K+-ATPase的活性的影响。结果表明：SFPS能明显抑制S180A小鼠,EAC小鼠,L615小鼠红细胞膜Na+,K+-ATPase的活性。本文结果提示,SFPS 的这一作用可能是其促进荷瘤小鼠红细胞免疫功能的机理之一。     

间歇性低氧及运动对大鼠红细胞膜Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活性的影响

Wistar雄性大鼠分为常氧对照组、常氧运动组、低氧对照组、低氧运动组.分别对常氧运动、低氧运动组进行游泳训练,对低氧对照、低氧运动组进行低氧刺激.用导管法测血压和心率,用比色法测红细胞膜Na+-K+-ATP酶Ca2+-Mg2+-ATP酶活性,探讨低氧和训练对红细胞膜Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活性的影响.发现低氧刺激降低大鼠红细胞膜Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶的活性,而适宜负荷运动则可提高以上两种酶的活性.结果表明,间歇性低氧运动对大鼠红细胞膜的Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活性具有一定的保护作用.     

芦荟多糖对荷瘤小鼠肿瘤细胞钠泵的影响

报道了库拉索芦荟多糖、木立多糖对S1 80 小鼠肿瘤细胞膜Na+ ,K+ -ATPase的活性影响。结果表明 ,二者均能明显抑制荷瘤小鼠肿瘤细胞膜上Na+ ,K+ -ATPase的活性。芦荟多糖的这一作用改变了荷瘤小鼠细胞膜的物质、能量平衡 ,进而降低了肿瘤细胞的恶性程度。     

秦皮乙素对S180小鼠肿瘤细胞膜功能的影响

研究秦皮乙素对S_(180)小鼠肿瘤细胞膜功能的影响.采用抑瘤实验检测秦皮乙素对S_(180)小鼠体内抗肿瘤活性;采用荧光光度法检测秦皮乙素对S_(180)小鼠肿瘤细胞膜流动性的影响;采用比色法检测秦皮乙素对S_(180)小鼠肿瘤细胞膜Na~+,K~+-ATPase和Ca~(2+),Mg~(2+)-ATPase活性、含唾液酸量、封闭度的影响.秦皮乙素各组小鼠平均瘤重均低于阴性对照组,均可降低肿瘤细胞膜流动性、Na+,K+-ATPase和Ca~(2+),Mg~(2+)-ATPase活性和膜封闭度,均可抑制肿瘤细胞膜唾液酸的生成.秦皮乙素抑制肿瘤细胞的生长与改变肿瘤细胞膜功能有关.     

过度训练对红细胞膜结构和功能影响的实验研究

为进一步探讨过度训练的发生机制,采用建立一般训练和过度训练动物模型,应用荧光法和比色法分别测定了过度训练后红细胞膜MDA含量、红细胞变形性、Na~+-K~+-ATPase、Ca~(2+)-ATPase活性以及红细胞膜总磷脂、胆固醇含量及其比值.结果显示:与对照组相比,MDA含量在运动后即刻显著升高(P<0.01);红细胞变形性显著下降(<0.05;P<0.01),Na~+-K~+-ATPase和Ca~(2+)-ATPase活性显著下降(P<0.05),红细胞膜总磷脂显著下降(P<0.05),红细胞膜上胆固醇在运动后即刻显著低于对照组(P<0.01),红细胞膜上胆固醇/磷脂比值在运动后即刻显著低于对照组(P<0.05),相关分析表明,红细胞的变形性与红细胞膜上MDA含量呈显著负相关(P<0.05).结果揭示:过度训练运动引发的自由基产生的脂质过氧化对红细胞膜结构有损害,使Na~+-K~+-ATPase和Ca~(2+)-ATPase活性下降,红细胞膜上的磷脂和胆固醇含量以及其比值降低,致使红细胞变形性下降,可能是造成过度训练后红细胞功能下降的重要原因之一.     

大负荷跑台运动对大鼠心肌细胞膜Na+-K+-ATPase、Ca2+-ATPase与肌浆网Ca2+-ATPase活性的影响

目的:探讨大负荷跑台运动对大鼠心肌细胞膜钠钾泵(Na+-K+-ATPase)、钙泵(Ca2+-ATPase)与肌浆网钙泵(Ca2+-ATPase)活性的影响。方法:选择30只成年雄性SD大鼠分为空白对照组和运动后24 h组以及运动后即刻组。结果:运动后即刻组的Na+-K+-ATPase、Ca2+-ATPase活性均显著低于其他两组,(P<0.05);运动后24 h组的Ca2+-ATPase显著低于其他两组,(P<0.05);电镜观察可见运动后24 h组的肌细胞及肌浆网出现肿胀,肌原纤维结构出现明显的损伤;运动后即刻组的线粒体结构出现明显的损伤。结论:大负荷跑台运动会导致大鼠心肌细胞膜钠钾泵(Na+-K+-ATPase)、钙泵(Ca2+-ATPase)与肌浆网钙泵(Ca2+-ATPase)活性的降低。     

2型糖尿病肾病患者红细胞膜上钠-钾ATP酶活性的变化

为了解2型糖尿病肾病(diabetic nephropathy,DN)不同时期红细胞膜上Na+-K+-ATP酶活性变化,为临床诊断、治疗提供理论依据,按尿白蛋白排泄率(urinary albumin excretion rate,UAER)将已确诊为2型糖尿病的51例患者分为3组糖尿病正常白蛋白尿组(DM1组),微量白蛋白尿组(DM2组),大量白蛋白尿组(DM3组);20例健康人作为对照组,男性11例,女性9例,平均年龄58.27±10.89岁.将对照组及实验组抽血做红细胞膜Na+-K+-ATP酶活性检测.结果表明DN各组红细胞膜Na+-K+-ATP酶活性较对照组均显著降低(均为P=0.000).说明DN患者红细胞膜Na+-K+-ATP酶活性下降,控制血糖、维持Na+-K+-ATP酶活性对改善DN预后有十分重要的意义.     

锌-金属硫蛋白对力竭运动大鼠肝脏和股四头肌线粒体ATPase损伤的保护作用

通过锌-金属硫蛋白(Zn-MT)的补充干预观察对大鼠肝脏和股四头肌线粒体ATPase的影响,探讨Zn-MT对线粒体ATPase保护作用的机制.用雄性SD大鼠30只,随机分为安静对照组、运动对照组、补充Zn-MT+运动组.按Bedford运动模型运动至力竭后即刻处死,取肝脏和股四头肌制备线粒体备测.测定肝脏和股四头肌线粒体Na+,K+-ATPase、Ca2+-ATPase,Mg2+-ATPase活性,并进行有关统计学检验.结果表明,Zn-MT可以抑制由于力竭运动造成的肝脏和股四头肌中Na+,K+-ATPase、Ca2+-ATPase,Mg2+-ATPase活性的下降.     

秦皮乙素对荷瘤小鼠红细胞免疫功能的影响

研究秦皮乙素对S_(180)荷瘤小鼠红细胞免疫功能的影响,探讨秦皮乙素红细胞免疫抗肿瘤机制.采用体内移植S_(180)肿瘤细胞株复制S_(180)荷瘤小鼠模型.以红细胞免疫花环实验检测红细胞免疫黏附肿瘤细胞的能力;采用分光光度法测定荷瘤小鼠红细胞膜唾液酸含量、Na~+,K~+-ATP酶和Ca~(2+),Mg~(2+)-ATP酶活性.采用Western blot测定带3蛋白含量.结果表明:秦皮乙素能够增强S_(180)荷瘤小鼠红细胞免疫黏附肿瘤细胞的能力;增加红细胞膜唾液酸含量及带3蛋白含量;提高红细胞膜Na~+,K~+-ATP酶和Ca~(2+),Mg~(2+)-ATP酶活性.秦皮乙素可能通过改善S_(180)荷瘤小鼠红细胞膜的功能状态,提高膜的稳定性,增强红细胞免疫黏附肿瘤细胞的能力,从而发挥其抗肿瘤作用.     

植物细胞膜H -ATPase对必需矿质养分的适应性

植物细胞膜H -ATPase是初级转运蛋白,在各种离子和代谢产物的跨膜运输中起着重要的作用;介绍了植物细胞H -ATPase的结构、生化特性、生理功能与调控机制;重点综述了细胞膜H -ATPase的活性及其蛋白数量与基因表达对植物必需矿质养分的适应性.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Jane Eyre——A Feminist In The 19th Century

In the 19th century the society was controlled by men, and women were just appendants of them, they had not any rights and freedom. But Jane was an exception, she showed some characteristics of early feminist. Jane showed her characteristics of feminism in three aspects: rebellion, equality, and independence. These characteristics were helpful to her success, and feminism is the only way out for women of that time.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of