一种新的Bax异构体Baxθ促进细胞凋亡(英文)

Bcl-2蛋白家族由一类含有特征性的Bcl-2同源结构BH、且主要调控细胞死亡的蛋白组成.Bax是一个含有多个特征性BH结构域、促进凋亡的蛋白.克隆了一个全新的Bax异构体Baxθ,其cDNA由外显子1,4,5,6组成.MTC表达谱分析显示Baxθ在人体各组织中广泛且不同强度的表达.将BaxθORF插入pEGFP-N1载体,转染HEK293细胞,观察到一个15 ku的融合蛋白,提示Baxθ编码的蛋白是与Baxβ共用同一个C端,且使用一个全新的翻译起始位点.尽管Baxθ缺乏BH3结构域,但它在HEK293细胞中过表达时能促进细胞凋亡.截断体实验分析发现Baxθ第43~69位氨基酸残基可能与其促进细胞凋亡的活性有关.BH3结构域可能不是Bcl-2蛋白家族诱导细胞凋亡的唯一功能区域.     

麻疯树P5CS基因的克隆及原核表达

使用RACE技术,从麻疯树叶片中克隆了Δ1吡咯啉-5-羧酸合成酶(P5CS)基因,命名为JcP5CS. JcP5CS的cDNA全长2675 bp,包含一个2148 bp的开放阅读框,一个117 bp的5’-非翻译区以及一个410 bp的3’-非翻译区.开放阅读框编码716个氨基酸多肽链,分子量为77.54 kD,预测等电点为6.11.通过与其它物种的P5CS氨基酸序列比对,结果显示,与其他高等植物的P5CS氨基酸序列有很高的同源性.包含谷氨酸激酶和谷氨酸-γ-半醛脱氢酶两个结构域以及ATP及NAD (P     

中华绒螯蟹内源性白斑综合症病毒基因EjsWSSV的克隆与鉴定(英文)

基于已获得的表达序列标签序列,应用RACE技术从中华绒螯蟹基因组中获得了一个白斑综合症病毒基因(EjsWSSV)的全长cDNA序列,全长为3 864 bp并包含一个3 732 bp的开放阅读框,编码一个138.25 kDa的含1 243个氨基酸的多肽,编码的氨基酸序列包含一个VWA结构域.通过序列比对、序列结构比较和一些生物信息学预测分析,表明EjsWSSV蛋白序列与白斑综合症病毒基因组的ORF16具有高同源性,它不含有信号肽和跨膜区,为非分泌型和α-螺旋型蛋白,可能定位于细胞质.通过PCR检测验证,EjsWSSV基因可能为内源性病毒基因.     

枸杞抗坏血酸氧化酶基因的克隆与表达分析

以枸杞为材料,利用同源克隆技术,克隆了枸杞抗坏血酸氧化酶(AO)的全长cDNA序列,命名为LcAO基因(GenBank:KP712033),该基因开放阅读框(ORF)大小为1737bp,编码578个氨基酸,与西红柿AO蛋白的同源性达到90%.LcAO编码的氨基酸序列包含3个多铜氧化酶结构域和跨膜信号序列.采用实时荧光定量PCR分析显示,LcAO基因在枸杞的花和果实中表达量最高,成熟叶中表达量最少.     

大豆GmNF-YA8的生物信息学及盐胁迫表达分析

本实验对大豆GmNF-YA8基因进行了生物信息学分析和高盐胁迫下表达量的检测.GmNF-YA8位于大豆基因组9号染色体上,cDNA全长1121 bp,开放阅读框615bp,编码204个氨基酸.GmNF-YA8蛋白分子量22.7 kD,pI 6.16.GmNF-YA8蛋白氨基酸序列中含有1个CBF保守结构域.GmNF-Y...     

中华补血草金属硫蛋白基因的克隆及高盐处理下的表达模式分析

以中华补血草叶片为材料,提取其总RNA并反转录cDNA,并以cDNA为模板,克隆得到包含该基因完整开放阅读框的cDNA序列,序列分析表明,该基因开放阅读框长249 bp,编码82个氨基酸;该蛋白含有14个半胱氨酸,主要分布在蛋白质的N端和C端,呈CC,CX,CXXC形式排列.预测该蛋白分子质量为8 133.1 D;等电...     

白菜型油菜RAV基因的克隆及表达分析

基于拟南芥RAV类转录因子的序列,通过RACE方法获得白菜型油菜RAV基因的cDNA序列,全长1 306bp,其中包括5′-UTR109bp,3′-UTR171bp,开放阅读框1 026bp,编码341个氨基酸,预测蛋白分子量为38.03u,理论等电点为9.2,含有相对保守的AP2和B3结构域.多序列比对和系统进化分析表明,油菜RAV与拟南芥RAV1具有很高的一致性,为88.0%.实时荧光定量PCR结果表明,油菜RAV基因受低温和盐胁迫的诱导,推测该基因在响应非生物胁迫中发挥重要作用.     

利用酵母双杂交系统筛选与BnSmD1相互作用的蛋白

本研究以油菜BnSmD1为诱饵蛋白,利用酵母双杂交技术,从甘蓝型油菜cDNA文库中筛选出与之有相互作用的Arf-GTP酶激活蛋白1(Brassica napus ADP-ribosylation factor GTPase activating protein1,BnArf GAP1),采用RT-PCR扩增并克隆到3个油菜Arf GAP基因的开放阅读框全长序列,BnArf GAP1、BnArf GAP2和BnArf GAP3.BnArf GAP1和BnArf GAP2都拥有1个507bp的开放阅读框,编码168个氨基酸残基;与前两个基因相比,BnArf GAP3核苷酸序列中插入了一个93bp的带有终止密码子的外源片段,导致翻译的提前终止,它拥有1个249bp的开放阅读框,编码82个氨基酸残基.BnArf GAP1和BnArf GAP2核苷酸序列一致性达到98.22％;BnArfGAP1与BnArf GAP3核苷酸序列一致性达到82.83％,BnArf GAP2与BnArf GAP3核苷酸序列一致性达到84.17％.BnArf GAP1、BnArf GAP2和BnArf GAP3都含有一个C2结构域.     

蜘蛛抱蛋凝集素（Aspidistra elatior Bulme Lectin）的基因克隆及序列分析

运用同源克隆的方法设计简并引物,通过3’和5’RACE技术,从蜘蛛抱蛋（Aspidistra elatior Bulme）中克隆了编码甘露糖结合集素（Aspidistra elatior Bulme Lectin,AEL）的全长cDNA序列.其cDNA全长为1149 bp,包含930 bp的开放阅读框,编码309个氨基酸的前体蛋白.前体蛋白经翻译后剪切成分子量分别为12.31 kD 和12.26 kD两个成熟蛋白亚基（AELDOM1,AELDOM2）.AELDOM1和AELDOM2分别具有     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.