Enzymatic activity (three dehydrogenase systems) of the pharyngeal tumour in the earthwormLumbricus terrestris

Zusammenfassung Beschreibung von Riesenkernen und einer Geschwulst des Pharynx beiLumbricus terrestris.     

Enzymatic activity (three dehydrogenase systems) of the pharyngeal tumour in the earthwormLumbricus terrestris

Zusammenfassung Drei Dehydrogenase-Systeme (Succino-, DPN- und TPN-Dehydrogenase sowie einige DPN-ähnliche Dehydrogenasen, wie die auf Milch-, Malon-, Glutamin- und -Hydroxybuttersäure sowie auf Äthanol wirkenden substrat-spezifischen Dehydrogenasen) wurden an einer Geschwulst des Pharynx des RegenwurmesLumbricus terrestris untersucht.Die Enzymaktivität war für die DPN- und die Milchsäure-Dehydrogenase sehr hoch; eine mittlere Aktivität zeigten die TPN-, die Malonsäure- und die -Hydroxybuttersäure-Dehydrogenase. Sehr gering war die Reaktion für die Succino- sowie die Glutaminsäure-Dehydrogenase, während sie für die Äthanol-Dehydrogenase negativ ausfiel.     

Origin of the differences of superficial potentials in Rana esculenta]

The spatial distributions of superficial D.C. potentials on the skin of Rana esculenta have been compared to those of the intensity of short-circuit current (S.C.C.) expressing the transcutaneous active transport of sodium ions. It has been observed that the sites of maximum D.C. potentials coincide with the localisations of maximum S.C.C. values. Moreover, superficial D.C. potentials and S.C.C. are similarly modified by the depression of metabolic activity due to lowered temperature or poisoning by dinitrophenol (DNP). It is thus proposed that the spatial distribution of the transcutaneous active transport system for sodium ions is the origin of the electric generator of superficial D.C. potentials.     

Specific inhibition of formation of acid-fastness in mycobacteria by 3,3'-di-O-methylellagic acid.

3,3'-Di-O-methylellagic acid obtained from Euphorbia adenochlora selectively inhibited the formation of acid-fastness in mycobacteria without retardation of their growth. Gross reductions in contents of wax D, cord factor and free mycolic acids were found in the nonacid-fast bacilli compared with the normal ones.     

Serum protein differences between ambulatory and bed-ridden subjects]

Total proteins as well as 7 individual protein components were evaluated in the blood serum of 19 subjects, first ambulatory, then recumbent. A concentration decrease of 7% has been found for the latter, independently of the nature of the various proteins studied.     

Hormonal induction of glutamate dehydrogenase in rat liver.

Glutamate dehydrogenase rapidly increases in microsomes and appears in the cytoplasm after administration of cortisone, cAMP, hydrocortisone-acetate. Prolonged administration of ACTH maintains high level of enzyme in the mitochondria and microsomes. Hydrocortisone-acetate, insulin and cortisone decrease drastically enzyme in mitochondria.     

Lactate dehydrogenase isozymes of mouse epidermis.

Five isozymes of LDH are demonstrable in the epidermis of the ear pinnae, hind feet, trunk dorsa, and tails of adult C57BL, C57HR, and C3HB mice by polyacrylamide gel electrophoresis. LDH-5 activity predominates in electropherograms. The ratio of LDH-1 to LDH-K is greater in the epidermis of ear pinna and trunk dorsum than in that of tail and hind foot. The region-specific patterns of epidermal LDH isozymes are not correlated with melanin pigmentation or "hairiness' of the skin.     

Enzymatic characteristics of the catalytic subunit of the protein kinase complex of human lymphocytes]

In earlier reports we have shown the existence in human lymphocytes homogenate, of a cyclic-AMP dependent protein-kinase activity. We demonstrate by affinity chromatography that two subunits display respectively cyclic-AMP binding and phosphorylating properties. Divalent cations such as Ca++, Mg++ or Mn++ are required for enzymatic activity. ATP which is an obligatory cosubstrate acts as an inhibitor when its concentration is higher than 10(-6)M.     

Enzymatic patterns in reptilian brain. Histochemical characterization of the optic tectum

Summary The enzymatic patterns present in the optic tectum of 4 species belonging to different reptilian orders seem related to the degree of structural and functional organization reached by the nervous centre, as in other vertebrates. In particular the AChE localization in reptiles is representative of a evolutionary sequence in the distribution of this enzyme in the optic tectum along the tetrapode series.     

Sexual differences in the development of plasma thyroxine levels in the embryo and young chick]

Plasma thyroxine (T4) levels were separately ascertained in male and female embryo and young chicken from the 12th day of incubation till the 4th day after hatching by the thyroxine binding globulin technique. In both sexes, plasma T4 reach a peak the 20th day of incubation, but values are significantly higher in females. A sharp decrease occurred thereafter, plasma T4 tending toward adult values the 4th day after hatching.     

Regional distribution of lactate dehydrogenase isoenzymes in adult rat brain.

The highest lactate dehydrogenase (LDH) activity was found in thalamus, statistically significantly less in cerebral and cerebellar cortex and the lowest in pons. LDH1 and LDH4+5 represented 58% and 23% of the total activity in cerebral cortex, 54% and 20% in thalamus, 42% and 4% in cerebellar cortex and 55% and 7% in pons, respectively.     

Lactate dehydrogenase isozymes in xiphophorin fish melanoma conditioned by the locusSd.

Zusammenfassung Elektrophoretische Untersuchungen an benignen und malignen Rückenflossenmelanomen sowie an normalen Rückenflossen lebendgebärender Zahnkarpfen zeigen, dass in den Geweben in erster Linie die für gut mit Sauerstoff versorgtes Gewebe typische Herz-LDH (B₄-Isoenzym) vorkommt. Die für schlecht mit Sauerstoff versorgtes Gewebe typische Muskel-LDH (A₄-Isoenzym) wird nur in manchen der untersuchten Gewebe gefunden, und ist auch dort nur in sehr geringer Menge vorhanden. Die Melanome scheinen also im Gegensatz zu vielen anderen Neoplasmen anderer Systeme aeroben Stoffwechsel durchzuführen.

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Supported by Deutsche Forschungsgemeinschaft through Sonderforschungsbereich 103, Zellenergetik und Zelldifferenzierung (Marburg).

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Richard Merton—Guest Professor through Deutsche Forschungsgemeinschaft.     

Glucose-6-phosphate dehydrogenase activity in the ovaries ofScomber scomber L.

Résumé L'activité de la glucose-6-phosphate déshydrogénase a été étudiée histochimiquement dans les ovaires du maquereau. Les cellules thécales et folliculaires du follicule montrent une activité marquée de la glucose-6-phosphate déshydrogénase connue comme le plus important système engendrant la triphosphopyridine-nucléotide réduite, nécessaire pour la synthèse des hormones stéroides.