共查询到20条相似文献,搜索用时 718 毫秒
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Yeast activators stimulate plant gene expression 总被引:25,自引:0,他引:25
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Negative effect of the transcriptional activator GAL4 总被引:89,自引:0,他引:89
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Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins 总被引:1,自引:0,他引:1
Mutations in the fushi tarazu (ftz) locus of Drosophila result in embryos with half the usual number of body segments. The sequences of the wild-type gene, a temperature-sensitive allele and a dominant mutant allele are presented. A portion of the conserved protein domain present in ftz and several homoeotic genes resembles the DNA-binding region of prokaryotic DNA-binding proteins, and is also similar to products of the yeast mating-type locus. 相似文献
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Drosophila Krüppel protein is a transcriptional repressor 总被引:45,自引:0,他引:45
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GAL4-VP16 is an unusually potent transcriptional activator 总被引:187,自引:0,他引:187
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Direct homeodomain-DNA interaction in the autoregulation of the fushi tarazu gene. 总被引:11,自引:0,他引:11
A major problem in the elucidation of the molecular mechanisms governing development is the distinction between direct and indirect regulatory interactions among developmental control genes. In vivo studies have indicated that the Drosophila segmentation gene fushi tarazu (ftz) directly or indirectly autoregulates its expression. Here we describe a generally applicable experimental approach which establishes a direct in vivo interaction of the homeodomain protein ftz with the ftz cis-autoregulatory control region. In vitro studies have shown that the DNA-binding specificity of the ftz homeodomain can be changed by a single amino-acid substitution in the recognition helix (Gln 50----Lys). Whereas wild-type ftz homeodomain binds preferentially to a CCATTA motif, the mutant homeodomain (ftzQ50K) recognizes a GGATTA motif. We now find that the in vivo activity of an ftz autoregulatory enhancer element is reduced by mutations of putative ftz-binding sites to GGATTA. This down-regulatory effect is specifically suppressed in vivo by the DNA-binding specificity mutant ftzQ50K. These results establish a direct positive autoregulatory feedback mechanism in the regulation of this homeobox gene. 相似文献
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GAL4 activates transcription in Drosophila 总被引:32,自引:0,他引:32
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Oncogene jun encodes a sequence-specific trans-activator similar to AP-1 总被引:166,自引:0,他引:166
P Angel E A Allegretto S T Okino K Hattori W J Boyle T Hunter M Karin 《Nature》1988,332(6160):166-171