肝癌细胞对三氧化二砷诱导凋亡的敏感性与细胞内谷胱甘肽含量的关系

目的:比较肝癌BEL-7402和SMMC-7721细胞对三氧化二砷(As2O3)诱导凋亡的敏感性差异,探讨细胞内谷胱甘肽(GSH)含量与其作用的关系。方法:不同浓度As2O3作用BEL-7402和SMMC-7721细胞24~96 h,流式细胞仪检测细胞DNA含量,计算细胞凋亡百分率;GSH检测试剂盒检测细胞内GSH含量。结果:0.25μmol/L As2O3作用24 h,有效地诱导BEL-7402细胞凋亡;对SMMC-7721细胞来说,需要用1.0μmol/L As2O3作用24 h才能达到相同的抑制效果。1.0μmol/LAs2O3作用72 h时,BEL-7402细胞的凋亡率为(43.8±0.2)%,SMMC-7721细胞的凋亡率为(12.8±0.2)%,检测BEL-7402细胞内GSH为(18.7±1.4)nmol/mg;SMMC-7721细胞内GSH为(50.8±5.2)nmol/mg,两者比较均有显著差异。结论:肝癌BEL-7402和SMMC-7721细胞对As2O3诱导细胞凋亡的敏感性不同,可能与细胞内GSH含量有关。     

姜黄素促进紫杉醇抑制肿瘤细胞的增殖

培养的人喉癌细胞 Hep‐2及肝癌细胞HepG‐2,分别用不同浓度姜黄素（CUR ,0～50μmol／L）和紫杉醇（PTX ,0～5 nmol／L ）单独处理或联合处理（5μmol／L CUR＋0．5 nmol／L PTX、10μmol／L CUR＋0．5 nmol／L PTX）．处理48 h后,用WST‐1细胞增殖试剂盒检测细胞增殖情况,再用结晶紫染色检测活细胞的密度,最后用Hoechst 33258凋亡染色试剂盒检测细胞凋亡情况．结果显示：姜黄素或紫杉醇单独处理对 Hep‐2细胞和 H epG‐2细胞的增殖均有抑制作用,并呈现出一定剂量效应关系;姜黄素与紫杉醇联合处理后细胞的增殖速率要比紫杉醇单独处理的明显降低;同时,姜黄素与紫杉醇联合处理后细胞的凋亡要比紫杉醇单独处理的更为明显．上述研究结果说明,姜黄素对紫杉醇抑制肿瘤细胞H ep‐2和H epG‐2的增殖有促进作用,并能促进紫杉醇诱导细胞的凋亡．     

三氧化二砷联合Trolox对人早幼粒白血病细胞HL-60增殖和凋亡的影响

利用MTT法、台盼蓝排染法、Hoechst33258荧光染色法、瑞氏-姬姆萨染色法以及单细胞凝胶电泳法检测了三氧化二砷(As2O3)单独作用及联合Trolox后对人早幼粒白血病细胞HL-60的增殖抑制、凋亡诱导作用及其DNA损伤作用.结果表明,1μmol.L-1As2O3作用即可抑制HL-60细胞的增殖,联合100μmol.L-1Trolox对HL-60细胞增殖抑制作用较As2O3单独作用时明显增强.高浓度的As2O3(4μmol.L-1)作用24h可引起HL-60细胞的凋亡,与Trolox联合作用,在低浓度(1μmol.L-1)下即可引起细胞凋亡.联合100μmol.L-1Trolox可以显著增强As2O3引起的HL-60细胞的DNA损伤.这种损伤可能导致细胞周期阻滞,从而在生长曲线中体现出对HL-60细胞的增殖抑制作用.     

15-KETE对大鼠肺动脉平滑肌细胞上ERK1／2活性的影响

目的从细胞分子水平上研究15-酮基二十碳四烯酸（15-ketoeicosatetretraenoicacid,15-KETE）对大鼠肺动脉平滑肌细胞上ERKl／2的活性的影响。方法通过酶法分离、培养SD大鼠肺动脉平滑肌细胞（pulmonaryarterialsmoothcells,PASMCs）,使用Westernblot技术观察15-KETE对大鼠PASMCs上ERKl／2活性的影响。结果1）10～mol／L15-KETE作用从0．5h到24h与对照组（未加15-KETE）比较,对ERKl／2总量没有明显的影响。2）与对照组比较,15-KETE处理0．5、1．0、2．0、4．0、8．0h明显增强P—ERKl／2（P〈0．05）。3）与对照组比较,10、10^-7和10～mol／L15-KETE明显增强p-ERKl／2（P〈0．05）,随15-KETE浓度增大,p-ERKl／2增多。4）与15-KETE作用比较,ERKI／2抑制剂PD9805920μmol／L、50txmol／L和U01260．1txmol／L、1btmol／L均能抑制15-KETE对ERKl／2的活化（P〈0．05）,并且随着抑制剂浓度增大,抑制作用加强。结论15-KETE对大鼠肺动脉血管平滑肌细胞上的ERKl／2表达没有明显的影响,但能激活肺动脉血管平滑肌细胞ERKI／2．使其磷酸化,并呈时间一剂量依赖关系。ERKl／2通路的特异性抑制剂PD98059、U0126能抑制15-KETE对ERKl／2的磷酸化。     

CdCl2诱导秋粘虫细胞(Sf9)凋亡的初步研究

以秋粘虫（Spodoptera frugiperda Sf9）的细胞为材料，用不同浓度的CdCl2，通过Giemsa染色的形态学方法，研究其细胞凋亡．结果表明，在重金属离子的胁迫作用下，不同浓度的重金属离子致使细胞死亡率不同；其急性损伤域为136μmol／L；20μmol／L CdCl2作用6h后，Sf9细胞出现了凋亡小体．     

槲皮素(Quercetin)抑制胆管细胞癌HCCC-9810细胞上皮-间质转化(EMT)并上调抗肿瘤药物对其杀伤作用

背景 胆管细胞癌是原发性肝癌的一种,是目前肝癌诊疗的最大难点。目的 检测槲皮素（Quercetin）对抗肿瘤药物作用于人肝内胆管细胞癌细胞系HCCC-9810的影响,确定其在胆管癌细胞治疗中的潜在作用。方法 使用系列浓度梯度的槲皮素作用于HCCC-9810细胞,使用MTT法计算抑制率,确定槲皮素作用于HCCC-9810细胞的量-效关系。检测系列浓度梯度的抗肿瘤药物索拉菲尼（Sorafenib）、奥沙利铂（Oxaliplatin）和多西他赛（Docetaxel）对HCCC-9810细胞的杀伤作用,确定抗肿瘤药物作用的量-效关系。选取适宜的作用浓度,利用MTT法检测槲皮素对上述抗肿瘤药物杀伤HCCC-9810细胞的影响。利用蛋白印迹实验,检测槲皮素对上皮细胞标识物E-Cadherin以及间质细胞标识物N-Cadherin、Vimentin表达的影响。结果 槲皮素、索拉菲尼、奥沙利铂和多西他赛等能够剂量依赖地抑制HCCC-9810细胞的存活;选取2μmol/L为后续实验的槲皮素作用浓度,该浓度的槲皮素预处理HCCC-9810细胞能够显著上调其对抗肿瘤药物的敏感性,索拉菲尼、奥沙利铂和多西他赛作用于HCCC-9810细胞的半数作用浓度（IC50）依次从2.75±0.22μmol/L、0.83±0.12μmol/L和0.05±0.01μmol/L下调为0.31±0.5μmol/L、0.15±0.02μmol/L和0.01±0.00μmol/L。作用机制实验结果表明,槲皮素处理能够下调HCCC-9810细胞中的间质细胞标识物N-Cadherin和Vimentin的表达,上调上皮细胞标识物E-Cadherin的表达。结论 槲皮素能够通过抑制胆管癌细胞系EMT上调抗肿瘤的效能,在肝脏胆管细胞癌诊疗中具有潜在应用价值。     

羟基喜树碱联合丝裂霉素诱导人白血病K562细胞凋亡

观察羟基喜树碱与丝裂霉索联合应用对人白血病细胞K562的作用效果,并探讨其机制。不同浓度羟基喜树碱与丝裂霉素单独及联合作用于人白血病细胞K562后,应用台盼蓝拒染法检测细胞生长抑制率,计算合用指数（CI）,流式细胞仪（FCM）检测K562细胞凋亡率,吖啶橙（AO）荧光染色和透射电镜观察凋亡形态学变化。结果表明：单独应用时羟基喜树碱和丝裂霉素的IC50分别是8μ/mL和12．5μg/mL,联合应用时IC50下降为4μg/mL（HCPT）和3．6μg/mL（MMC）,CI=0．78,为协同效应。羟基喜树碱与丝裂霉素单独及联合应用均可诱导K562细胞凋亡。2种药物联合应用时的凋亡率高于各自单独用药。羟基喜树碱与丝裂霉素联合应用可以通过共同诱导细胞凋亡。协同抑制人白血病细胞生长。     

玫瑰茄细胞生长及其花黄素产生的研究

本文对玫瑰茄细胞培养产生产花黄素进行了研究。实验结果表明，细胞在含1μmol/L2,4-d及0．5μmol/LKT的B5悬浮培养基中生长良好，有产生较多的花黄素，为172mg/L，占细胞干重的2．1％。细胞的比生长速率为0．．282d^-1，倍增时间为59h，色素产生的动力学方程为：dP/dt=(3.333 0.1830μ）x。2，4－D的浓度升高对色素的产生有抑制作用。蔗糖的最适浓度为3％。培养基中有无Fe^ 对产物形成的影响作用极微，而光照对细胞生长和色素形成都是必需的。     

依地福新抑制Jurkat细胞生长机制的研究

目的观察依地福新对体外培养的Jurkat细胞的生长抑制作用,并进一步探讨其作用机制。方法应用MTT法检测细胞增殖活性;流式细胞仪检测细胞周期分布;Annexin V—FITC染色法检测细胞凋亡率。结果不同浓度依地福新处理Jurkat细胞24～96h后,细胞增殖显著受到抑制,并呈现浓度及时间依赖性;1．0μmol／L、5．0ymol／L、10．0μmol／L依地福新处理72h后,Jurkat细胞G0／G1期细胞数量显著增加,S期细胞数量显著降低（P〈0．01）;各浓度组细胞凋亡率均显著增加（P〈0．01）。结论依地福新对Jurkat细胞具有生长抑制作用,其机制与阻滞细胞周期及诱导凋亡有关。     

三氧化二砷抑制肝癌细胞端粒酶活性的实验研究

目的：观察三氧化二砷对肝癌细胞株BEL-7402和SMMC-7721端粒酶活性的影响。方法：应用端粒酶多聚酶联反应一酶联免疫测定(PCR-ELISA)方法，检测三氧化二砷作用后，肝癌细胞系BEL-7402和SMMC-7721细胞端粒酶活性的变化并比较两种细胞端粒酶对三氧化二砷敏感性的差异。结果：0．25～2．00μmol／L三氧化二砷(24～96h)可抑制肝癌细胞系BFL-7402的端粒酶活性，抑制作用与时间、剂量呈依赖性关系；0．25～0．50μmol／L三氧化二砷对SMMC-7721细胞端粒酶活性没有影响(96h以内)，1．00～2．00μmol/L三氧化二砷可抑制SMMC-7721细胞端粒酶活性(4H8～96h)，有时间、剂量依赖关系。结论：三氧化二砷可以抑制肝癌细胞系BEL-7402和SMMC-7721细胞端粒酶的活性；BEL-7402细胞端粒酶对三氧化二砷的敏感性较SMMC-7721细胞端粒酶高。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the