(S)-萘普生分子印迹聚合物结合特性及制备中复合物的性能研究

以丙烯酰胺为功能单体，合成了对(S)-萘普生具有高手性选择性的分子印迹聚合物．通过紫外光谱分析了聚合物制备过程中(S)-萘普生与功能单体丙烯酰胺形成的复合物，并用Hyperchem软件模拟其结构．分子印迹聚合物对模板分子的结合量高于其对映异构体，手性分离因子α达1．87．Scatchard分析表明分子印迹聚合物在识别(S)-萘普生分子过程中存在2类结合位点，计算得到高亲和力的结合位点的离解常数Kd．1和最大表观结合常数Qmax.1分别为28．0μmol／L和52．94μmol／g，低亲和力的结合位点的离解常数Kd．2和最大表观结合常数Qmax,2分别为0．489mmol／l和0．122mmol／g．     

美托洛尔分子印迹聚合物识别性能的研究

采用紫外光引发的分子印迹技术,以美托洛尔为模板,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,成功制备了以美托洛尔(Metoprolol)为模板的分子印迹聚合物.结合底物的实验表明,该聚合物对美托洛尔具有优良的吸附选择性.并通过Scatchard方程研究了印迹聚合物对模板分子的结合特性,结果表明,功能单体甲基丙烯酸与模板分子在自组装过程中通过氢键和离子键形成了2类非等价的结合位点,并计算出2类不同结合位点的离解常数分别为Kd1=2.398×10-4mol/L,Kd2=6.364×10-4mol/L;最大表观结合量Qmax1=58.57μmol/g;Qmax2=86.13 μmol/g;分别为理论吸附量的50.53%和74.31%.     

利福平分子印迹聚合物微球的制备及性能研究

以聚乙烯醇1735为分散剂、利福平为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂,用水溶液微悬浮聚合法制备了利福平分子印迹聚合物微球;在水溶液中,利用静态平衡结合法和Scatchard分析法研究了印迹聚合物的结合能力和选择性.结果表明,利福平溶液的浓度范围在5×10-5~1×10-4mol/L内,所制得的印迹聚合物内存在两类不等价的结合位点,根据实验数据计算得到高亲和力结合位点的离解常数和最大表观结合量分别为Kd1=1.08×10-2mg/mL和Qmax1=14.40 mg/g;低亲和力结合位点的离解常数和最大表观结合量分别为Kd2=7.18×10-3mg/mL和Qmax2=12.51 mg/g.结合底物的实验结果表明,利福平分子印迹聚合物微球对利福平呈现出了较高的吸附性能和选择识别特性,能较简便地用于利福平的分离和富集.     

配合物分子印迹聚合物的识别性能

以乙酸镍和2,2′-联吡啶配合物为模板、4-乙烯吡啶为功能单体、乙二醇二甲基丙烯酸酯为交联剂,合成了具有类似金属螯合抗体结合位点的金属配合物印迹聚合物,系统研究了金属离子对模板聚合物选择性结合2,2′-联吡啶的调节作用。结果表明:印迹聚合物对N i(Ⅱ)-2,2′-联吡啶配合物有选择性识别能力。采用Scatchard模型评价分子印迹聚合物的结合特性,得到高亲和力结合位点的平衡离解常数Kd1=0.082 mm o l/L,表观最大结合量Qm ax1=82.2μm o l/g,低亲和力结合位点的Kd2=0.400 mm o l/L,Qm ax2=91.3μm o l/g。     

微球形磺胺嘧啶分子印迹聚合物合成及识别特性

以磺胺嘧啶为模板分子,运用分子印迹技术制备微球形分子印迹聚合物,通过Scatchard模型.求得分子印迹聚合物结合位点的离解常数为8.6μmol/L,最大表观结合量为185.7,μmol/g.分子印迹聚合物的等温吸附线方程符合Longmuir方程.分子印迹聚合物的选择性吸附随着溶剂极性的增加而减小,表明非极性共价键在分子识别中起重要作用.     

SBA-15表面S-naproxen分子印迹聚合物微球的合成与分子识别特性

以手性药物左旋萘普生(S-naproxen)为模板分子,四乙烯基吡啶(4-VPy)为功能单体,采用表面印迹法,以介孔材料SBA-15为载体合成了能选择性识别S-naproxen的分子印迹聚合物微球.扫描电镜及孔结构分析结果表明,所合成的分子印迹聚合物微球具有粒径均匀、孔径分布窄、比表面积大等特点;同时扫描电镜、X射线衍射和红外光谱分析结果表明载体表面形成了分子印迹聚合物层,Scatchard分析表明分子印迹聚合物在自组装过程中存在两类结合位点,聚合物高亲和力和低亲和力结合位点的最大表观结合容量分别为Qmax1=2.504μmol/g,Qmax2=16.680μtmol/g;分子印迹聚合物的热力学研究表明,吸附过程可以自发进行.     

三唑醇分子烙印聚合物的吸附特性

实验采用分子烙印技术合成了对农药三唑醇有特异性作用的分子烙印聚合物(molecularly im-printed polymer,MIP).通过平衡吸附实验,评价了其对三唑醇的亲和力和选择性.与非烙印聚合物相比,分子烙印聚合物对三唑醇表现了较强的亲和力;Scatchard分析表明,以甲基丙烯酸为功能单体,以三唑醇为模板的分子烙印聚合物,通过离子作用和氢键作用可形成两类结合位点.用多点结合模型计算两类不同结合位点的离解常数为Kd1=2.80×10-4mol/L,Kd2=9.71×10-3 mol/L.实验表明,该聚合物对三唑醇有高亲和力和选择性,为在生物样品中选择富集三唑醇提供了可能性.     

微球形磺胺脒分子印迹聚合物的制备及其性能

建立了以沉淀聚合方式制备磺胺脒分子印迹聚合物微球的方法,其中磺胺脒用作模板分子,甲基丙烯酸用作功能单体,二甲基丙烯酸乙二醇酯用作交联剂.对印迹微球的选择性和吸附性能进行了研究.优化的反应物用量是:磺胺脒0.05mmol,甲基丙烯酸0.2mmol,二甲基丙烯酸乙二醇酯1mmol(摩尔比1∶4∶20).在此条件下,溶剂乙腈用量是5mL,引发剂偶氮二异丁腈用量是10mg,在60℃下热引发聚合反应24h,等温静态平衡实验结果表明,该印迹聚合物与相应的空白聚合物相比具有高的选择性和亲和性.Scatchard模型分析结果表明,该印迹聚合物在印迹过程中形成了2类不同的结合位点,高亲和性位点离解常数和最大表观吸附量分别为1.932mmol/L和196.06μmol/g,低亲和性位点离解常数和最大表观吸附量分别为23.843mmol/L和1 449.04μmol/g.印迹聚合物微球重复使用10次吸附性能无显著下降,具有作为固相萃取柱填料使用的潜力.     

氯嘧磺隆分子印迹聚合物的合成及其性能

建立了微球形氯嘧磺隆分子印迹聚合物的合成方法.优化了影响聚合物吸附性能的参数,对聚合物微球进行了性能表征.在氯嘧磺隆印迹微球的合成方法中,功能单体为甲基丙烯酸,交联剂为乙二醇二甲基丙烯酸酯,溶剂为乙腈,引发剂为偶氮二异丁腈,当其用量分别为0.25,1.5,5 mmol(物质的量比1:6:20)、30 mL和0.30 mmol时合成的分子印迹聚合物对氯嘧磺隆有最好的特异性吸附能力.运用Scatchard 方程测定了该聚合物的结合能力.结果表明,聚合物中具有两类亲和性吸附位点,高亲和性吸附位点的最大表现吸附量Qmax1为63.46 μmol/g,平衡常数Kd1为0.655 7 mmol/L;低亲和力吸附位点的最大表观吸附量Qmax2为437.085 μmol/g,平衡常数Kd2为11.00 mmol/L.聚合物微球在重复使用9次后吸附性能基本无下降.本制备方法具有无需研磨筛分即可得到分子印迹微球的特点,便于作为固相萃取柱和色谱柱的填料使用.     

2,4-二氯苯氧乙酸分子印迹聚合物的制备及识别特性

以2,4-二氯苯氧乙酸(2,4-D)为模板分子,α-甲基丙烯酸(MAA)为功能单体,偶氮二异丁腈(AIBN)为引发剂,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,制备了有特异性识别2,4-D功能的分子印迹聚合物.用紫外分光光度法对印迹聚合物的吸附性能进行了研究,并用高效液相色谱法对印迹聚合物的选择性进行了考察.用Scatchard法分析表明,该分子印迹聚合物通过氢键作用力结合,对2,4-D的最大表观吸附量Qmax为69.7μmol.g-1,平衡离解常数Kd为9.96×10-3mol.L-1.HPLC实验表明,与2,4-D结构类似的分子相比,印迹聚合物对2,4-D显示了很好的选择性.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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