PcF protein from <Emphasis Type="Italic">Phytophthora cactorum</Emphasis> and its recombinant homologue elicit phenylalanine ammonia lyase activation in tomato

The phytotoxic protein PcF (Phytophthora cactorum-Fragaria) is a 5.6-kDa cysteine-rich, hydroxyproline- containing protein that is secreted in limited amounts by P. cactorum, an oomycete pathogen of tomato, strawberry and other relevant crop plants. Although we have shown that pure PcF triggers plant reactivity, its mechanism of action is not yet understood. Here we show that PcF, like other known fungal protein elicitors involved in pathogen-plant interaction, stimulates the activity of the defense enzyme phenylalanine ammonia a key step in understanding the mechanism of action of PcF at a molecular level is knowledge of its three-dimensional structure, we overexpressed this protein extracellularly in Pichia pastoris. The preliminary structural and functional characterization of a recombinant PcF homologue, N4-rPcF, is reported. Interestingly, although N4-rPcF is devoid of proline hydroxylation and has four additional amino acid residues attached to its N terminus, its secondary structure and biological activity are indistinguishable from wild-type PcF.Received 22 February 2003; received after revision 25 March 2003; accepted 14 April 2003     

Host trail following by the guest antFormicoxenus provancheri

Formicoxenus provancheri, a guest ant ofMyrmica incompleta, is able to follow artificial trails made with the poison gland secretion of its host. The trail-following response is elicited at the same range of concentrations as for the host species. The performance ofFormicoxenus is enhanced by the presence of the host. The adaptive value of these phenomena is discussed.     

Newton’s De gravitatione: a review and reassessment

The widely accepted supposition that Newton’s De gravitatione was written in 1684/5 just before composing the Principia is examined. The basis for this determination has serious difficulties starting with the failure to examine the numerical estimates for the resistance of aether. The estimated range is not nearly nil as claimed but comparable with air at or near the earth’s surface. Moreover, the evidence provided most likely stems from experiments by Boyle, Hooke, and others in the 1660s and does not use evidence available in the late 1684. The document supports Newton’s contention that the aether medium incorporates very large voids thereby proving that body and space differ but does by no means completely reject its corporeal nature or eliminate its resistance. Newton’s use of the term inertia provides no conclusive evidence for a late date as often claimed and his definition of gravitas is difficult to reconcile with a late one.     

<Emphasis Type="Italic">Legionella pneumophila</Emphasis> — a human pathogen that co-evolved with fresh water protozoa

The bacterial pathogen Legionella pneumophila is found ubiquitously in fresh water environments where it replicates within protozoan hosts. When inhaled by humans it can replicate within alveolar macrophages and cause a severe pneumonia, Legionnaires disease. Yet much needs to be learned regarding the mechanisms that allow Legionella to modulate host functions to its advantage and the regulatory network governing its intracellular life cycle. The establishment and publication of the complete genome sequences of three clinical L. pneumophila isolates paved the way for major breakthroughs in understanding the biology of L. pneumophila. Based on sequence analysis many new putative virulence factors have been identified foremost among them eukaryotic-like proteins that may be implicated in many different steps of the Legionella life cycle. This review summarizes what is currently known about regulation of the Legionella life cycle and gives insight in the Legionella-specific features as deduced from genome analysis. Received 1 September 2006; received after revision 10 October 2006; accepted 22 November 2006     

Maligned for mathematics: Sir Thomas Urquhart and his Trissotetras

Thomas Urquhart (1611–1660), celebrated for his English translation of Rabelais’ Gargantua et Pantagruel, has earned some notoriety for his eccentric, putatively incomprehensible early book on trigonometry The Trissotetras (1645). The Trissotetras was too impractical to succeed in its own day as a textbook, since it lacked both trigonometric tables and sample calculations. But its current bad reputation is based on literary authors’ amplifications of the verdict prefaced to its 19th century reprinting by one mathematician, William Wallace, who lacked the background to appreciate the book’s historical context. Considering that context (including seventeenth century ‘copious’ prose, and medieval logic and ‘art of memory’), the bad reputation is undeserved: the book is mathematically clear, clever (e.g. in superimposing 16 problems into one diagram), and complete. The Trissotetras may thus be viewed as simply one more of Urquhart’s polymathic projects and involvements – which included education, rise of the middle class, religious and class conflicts, development of science and mathematics, search for patronage, universal language construction, and development of English prose – which serve to make him a lively and instructive intellectual Everyman for his time.     

Biochemical variation in theRana esculenta complex: A new hybrid form related toRana perezi andRana ridibunda

Summary Investigations of the green frogs from western Europe for electrophoretic variations at 4 enzyme loci demonstrated a new form which must be considered as a hybrid betweenRana ridibunda andR. perezi. Biochemical evidence supports the hypothesis that its reproduction is hybridogenetic, as it is forR. esculenta.     

Encapsulation ofPsorospermium haeckeli by the haemocytes ofAstacus leptodactylus

Summary Psorospermium haeckeli, a parasite of freshwater crayfish, has sometimes been observed encapsulated within the tissues ofAstacus leptodactylus. This observation does not agree with earlier reports. The lack of tolerance is discussed in relation to variations affecting either the parasite or its host.     

Über die farbgebende Gruppe von Crenilabrus-Blau

Summary The blue chromoproteid isolated from the fins ofCrenilabrus pavo C.V. is split by alkali hydrolysis into a protein and a pigment soluble in chloroform. The pigment is identified as biliverdin by its positive Gmelinreaction, the UV and visible absorption spectrum, and the chromatographic behaviour of its dimethyl ester. The lowest molecular weight of the chromoproteid has been evaluated from its biliverdin content to be 37,000±2400; different chromatographic fractions are presumably oligomeres of this unit.

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Herrn Professor Dr.Richard Kuhn zum 65. Geburtstag gewidmet.     

The superoxide-generating NADPH oxidase: structural aspects and activation mechanism

Flavocytochrome b ₅₅₈ is the catalytic core of the respiratory-burst oxidase, an enzyme complex that catalyzes the NADPH-dependent reduction of O₂ into the superoxide anion O₂ ^- in phagocytic cells. Flavocytochrome b ₅₅₈ is anchored in the plasma membrane. It is a heterodimer that consists of a large glycoprotein gp91phox (phox for phagocyte oxidase) (β subunit) and a small protein p22phox (α subunit). The other components of the respiratory-burst oxidase are water-soluble proteins of cytosolic origin, namely p67phox, p47phox, p40phox and Rac. Upon cell stimulation, they assemble with the membrane-bound flavocytochrome b ₅₅₈ which becomes activated and generates O₂ ^-. A defect in any of the genes encoding gp91phox, p22phox, p67phox or p47phox results in chronic granulomatous disease, a genetic disorder characterized by severe and recurrent infections, illustrating the role of O₂ ^- and the derived metabolites H₂O₂ and HOCl in host defense against invading microorganisms. The electron carriers, FAD and hemes b, and the binding site for NADPH are confined to the gp91phox subunit of flavocytochrome b ₅₅₈ . The p22phox subunit serves as a docking site for the cytosolic phox proteins. This review provides an overview of current knowledge on the structural organization of the O₂ ^--generating flavocytochrome b ₅₅₈ , its kinetics, its mechanism of activation and the regulation of its biosynthesis. Homologues of gp91phox, called Nox and Duox, are present in a large variety of non-phagocytic cells. They exhibit modest O₂ ^--generating oxidase activity, and some act as proton channels. Their role in various aspects of signal transduction is currently under investigation and is briefly discussed. Received 28 May 2002; received after revision 20 June 2002; accepted 24 June 2002     

Photodecomposition of orellanine and orellinine,the fungal toxins ofCortinarius orellanus Fries andCortinarius speciossimus

Summary The photosensitivity of orellanine, the main toxin ofCortinarius orellanus Fries mushrooms, and its transformation to orelline via orellinine is discussed. All three substances were found in methanolic extracts ofCortinarius orellanus andCortinarius speciossimus mushrooms. The problem of homogeneity of orellanine is also discussed.Acknowledgment. The authors wish to express their gratitude to Prof. Dr W. Steglich from the Institut für Organische Chemie und Biochemie der Universität Bonn (Federal Republic of Germany) for having provided them with a sample of dried material ofC. speciossisimus.     

Mass production ofSpirulina

Conclusions A large industrial production ofSpirulina and its products is possible already now, thanks to technological development. This production capacity and its excellent properties as a food have justified the effort to overcome the barriers of sanitary regulations in countries such as Mexico, Japan, Canada, the United States of America, France, Great Britain, the Federal Republic of Germany, New Zealand, Australia, Korea etc., where its consumption is expanding. In the forecoming years, the production ofSpirulina is expected to reach several thousands of tons yearly, which will initiate a massive production and consumption program.Spirulina, which Farrar⁹ calls glimpse of the Aztec food technology, will undergo a broad distribution and its alimentary benefits will be within everyone's reach.At present, Sosa Texcoco is ready to market 500 tons more in 1982, and is working on another expansion of 2000 tons per year which should be realized by 1983.     

Signal regulation by family conspiracy

The signal regulating proteins (SIRPs) are a family of ubiquitously expressed transmembrane glycoproteins composed of two subgroups: SIRPα and SIRPβ, containing more than ten members. SIRPα has been shown to inhibit signalling through a variety of receptors including receptor tyrosine kinases and cytokine receptors. This function involves protein tyrosine kinases and is dependent on immunoreceptor tyrosine-based inhibition motifs which recruit key protein tyrosine phosphatases to the membrane. Negative regulation by SIRPα may also involve its ligand, CD47, in a bi-directional signalling mechanism. The SIRPβ subtype has no cytoplasmic domain but instead associates with at least one other transmembrane protein (DAP-12, or KARAP). DAP-12 possesses immunoreceptor tyrosine-based activation motifs within its cytoplasmic domain that are thought to link SIRPβ to activating machinery. SIRPα and SIRPβ thus have complementary roles in signal regulation and may conspire to tune the response to a stimulus. Received 6 July 2000; revised 2 August 2000; accepted 5 August 2000     

A possible method for improving the efficacy of dapsone

Summary The antileprosy drug dapsone is unable to penetrate intactMycobacterium leprae in vitro, as determined by its effect on o-diphenoloxidase in the bacilli. When combined with the peptide polylysine, the sulfone drug passes through the bacterial cell membranes, and penetrates the enzyme protein, resulting in a 100% inhibition of its activity.     

Fish with a Different Angle: The Fresh-Water Fishes of Great Britain by Mrs Sarah Bowdich (1791–1856)

Since first appearance, reviews and accounts of The Fresh-Water Fishes of Great Britain (1828–1838) have been surprisingly few. All agree that this rare work is remarkable for its illustrations. Its importance as a whole in the history of ichthyology, however, is largely unknown, or ignored. This article therefore constitutes the first study of the textual and contextual significance of The Fresh-Water Fishes of Great Britain. By examining in chronological order where, and by whom, the work was first reviewed and referenced until the 1860s, the extraordinary contributions that its author, Sarah Bowdich, made to ichthyology at the forefront of the field in the late 1820s can better be appreciated. Indeed, this multiple evidence demonstrates Sarah Bowdich's merits as an ichthyologist of the first order, and as the first woman ichthyologist. But establishing the significance of The Fresh-Water Fishes of Great Britain for the history of ichthyology then raises a further question. Why has it and its author been so ignored or forgotten? By returning for answers to the fields of ichthyology already considered, the article proposes that Sarah Bowdich's different angles on fish offer lines of investigation that are still important for the field today.     

The karyotype and genome structure of the pirate perchAphredoderus sayanus (Aphredoderidae: Teleostei)

Summary The standard karyotype, genome size (DNA content), and genomic DNA base composition and distribution of the relict paracanthopterygian fish,Aphredoderus sayanus, were investigated. Several features of theA. sayanus genome appear to be derived rather than primitive conditions. These include a large number (at least 10 pairs) of bi-armed chromosomes, a low genome size, and high DNA asymmetry. This may indicate thatA. sayanus is not a typical paracanthopterygian fish in terms of its genome structure.     

Karyological pattern of two Chilean lizards species of the genusLiolaemus (sauria; iguanidae)

Summary The karyotypes of Chilean lizardsLiolaemus pictusandLiolaemus cyanogaster is described for the first time. Both species possess 34 chromosomes; 6 pairs of macrochromosomes and 11 pairs of microchromosomes. Karyologically it is possible to differenciate this species because the pair No. 2 is metacentric (m) inL. pictusand submetacentric (sm) inL. cyanogaster. It is shortly discussed the signification of formule 2n=34 for the species ofLiolaemus analized karyologically and its possible mechanism of acquisition.     

Transfer of secondary metabolites from the spongesDysidea fragilis andPleraplysilla spinifera to the mantle dermal formations (MDFs) of the mudibranchHypserlodoris webbi

The opisthobranch molluscHypselodoris webbi is able to select, among its potential preys, sponges chemically rich in furanosesquiterpenoids. The sequestered secondary metabolites act as defensive allomones against predators and are accumulated in some dorsal glands (MDFs). This transfer from sponges to MDFs has been proven by maintainingH. webbi together with some selected sponges in an aquarium for a prolonged period.     

β-Lactamases as models for protein-folding studies

This review traces some of the key features of the folding of β-lactamases and their relevance to the way proteins fold in general. Studies on the enzymes have highlighted the nature and role of equilibrium and transient condensed states. The kinetics of folding are multiphasic, and when monitored by acrylamide quenching of the tryptophan fluorescence, an early phase provides evidence for the transient accumulation of a nonnative intermediate involving burial of tryptophan in a nonpolar environment. Intermediate phases can be understood in terms of progressive folding of different parts of the molecule. The later, slow phases are associated with proline isomerization in the TEM-1 enzyme and, in its P167T mutant form, with isomerization from trans to cis of the E166 T167 peptide bond. Coupled with kinetic and X-ray crystallographic studies of the β-lactamase from Staphylococcus aureus and its D179Q mutant, it appears that the final stage of folding is that of collapse and packing of the Ω-loop on to the main body of the protein.     

Between Viète and Descartes: Adriaan van Roomen and the <Emphasis Type="Italic">Mathesis Universalis</Emphasis>

Adriaan van Roomen published an outline of what he called a Mathesis Universalis in 1597. This earned him a well-deserved place in the history of early modern ideas about a universal mathematics which was intended to encompass both geometry and arithmetic and to provide general rules valid for operations involving numbers, geometrical magnitudes, and all other quantities amenable to measurement and calculation. ‘Mathesis Universalis’ (MU) became the most common (though not the only) term for mathematical theories developed with that aim. At some time around 1600 van Roomen composed a new version of his MU, considerably different from the earlier one. This second version was never effectively published and it has not been discussed in detail in the secondary literature before. The text has, however, survived and the two versions are presented and compared in the present article. Sections 1–6 are about the first version of van Roomen’s MU the occasion of its publication (a controversy about Archimedes’ treatise on the circle, Sect. 2), its conceptual context (Sect. 3), its structure (with an overview of its definitions, axioms, and theorems) and its dependence on Clavius’ use of numbers in dealing with both rational and irrational ratios (Sect. 4), the geometrical interpretation of arithmetical operations multiplication and division (Sect. 5), and an analysis of its content in modern terms. In his second version of a MU van Roomen took algebra into account, inspired by Viète’s early treatises; he planned to publish it as part of a new edition of Al-Khwarizmi’s treatise on algebra (Sect. 7). Section 8 describes the conceptual background and the difficulties involved in the merging of algebra and geometry; Sect. 9 summarizes and analyzes the definitions, axioms and theorems of the second version, noting the differences with the first version and tracing the influence of Viète. Section 10 deals with the influence of van Roomen on later discussions of MU, and briefly sketches Descartes’ ideas about MU as expressed in the latter’s Regulae.