Carboxymethytl Pachymaram Up-Regulates Dendritic Cell＇s Function in Hepatitis B Virus Transgenic Mice

The effect of carboxymethytl pachymaram (CMP) on the function of dendritic cells(DCs) derived from spleens of hepatitis B virus transgenic mice are studied in vitro. The phenotypes of DCs are tested by flow cytometry (FCM), cytokines measured by ELISA. The expression of DCs’ phenotypes in HBV transgenic mice are low (CD80⁺CD11c⁺:59.12±11.53 vs 9.60±4.53, p<0.01; CD80⁺ MHC-II⁺: 44.86±12.31 vs 9.80±5.72, p<0.01, normal mice vs HBV transgenic mice), the ability of DCs stimulating T lymphocytes proliferation decreases (0.37±0.11 vs 0.20±0.11, p<0.05, normal mice vs HBV transgenic mice), levels of IL-12 and IFN-γ decrease whereas the level of IL-10 increases; CMP can enhance DCs’ ability of stimulating T lymphocytes proliferation, facilitate the secretion of IL-12 and IFN-γ, inhibit the secretion of IL-10, thus up regulates DCs function. The results show a good prospective use of CMP on the treatment of chronic hepatitis B. Biography: HOU Anji (1963–), male, Ph.D. candidate, Associate professor, research direction: clinical virology.     

B-Valued Dyadic Derivative

The principles of the new maximal operator H＊ we defined are discussed. We prove that it is bounded from martingale Hardy-Lorentz L^Xp.q[0,1） to the Lorentz L^Xp.q[0,1） for 1/2〈 p〈∞, 0〈~ q ≤ ∞, where X is any Banach space. When the Banach space X has the RN property, the sequence dnHnf converges to f a.e. Meanwhile the convergence in L^Xp norm for 1≤p〈∞ is a consequence of that the family functions K （n∈N） is an approximate identity.     

Genetic Algorithm-Based Approaches for Optimizing S-Boxes

0 Introduction Being as unique nonlinear components of block ci- pher algorithms, S-boxes provide the most important confusion effect, and directly influence the security of the algorithms. There are many ways to construct S-boxes[1-5]. On one hand, diffe…     

Elliptic Curve Scalar Multiplication with x-Coordinate

This paper gives a comprehensive method to do Elliptic Curve Scalar Multiplication with only x-coordinate. Explicit point operation formulae for all types of defining equations of the curves are derived. For each type of curve, the performance is analyzed. The formulae are applied in Montgomery Ladder to get scalar multiplication algorithm operated with only x-coordinate. The new scalar multiplication has the same security level and computation amount with protected binary scalar multiplication （PBSM） against side channel attack, and has the advantages of higher security and little memory needed.     

Effect of Nano red elemental selenium on GPx activity of broiler chick kidney cells<Emphasis Type="Italic">in vitro</Emphasis>

A new selenium source, Nano red elemental selenium (Nano-Se) was used to study the effect on the GPx activity of broiler chick kidney cells (BCKC)in vitro, Sodium selenite (Na₂SeO₃) and seleno-1-methionine (Se-Met) were used as the controls. The results showed that the effects of three kinds of Se forms on the GPx activity of BCKC were accordant (p>0.05) compared with each other at 0.01. 0.05 and 0.10 μmol/L Se concentrations treatments. In the range of 0.00–0.10 μmol/L Se concentrations, the GPx activity increased with elevation of Se concentrations in medium. For the three kinds of Se forms, the GPx activity reached the climax at 0.10 μmol/L Se concentration. At 0.20 and 0.30 μmol/L Se concentrations, the influnces of three kinds of Se forms were not accordant with one another. For Nano-Se, the GPx activity at 0.20 and 0.30 μmoi/L Se concentrations remained the same as that at 0.10 μmol/L Se concentration treatment. For Se-Met, the GPx activity at 0.20 μmol/L Se concentration treatment remained the same with 0.10 μmol/L treatment; the GPx activity at 0.30 μmol/L Se concentration treatment was declined significantly (p<0.05) compared with 0.10 or 0.20 μmol/L treatment. For Na₂SeO₃, the GPx activity falled gradually with Se concentration increasing from 0.10 μmol/L to 0.30 μmol/L, and at 0.30 μmol/L Se concentration treatment, the GPx activity was less than the original of BCKC. The results implicated, on the GPx activity of BCKCin vitro, the ranking of width range of the most suitable Se concentration for nutrition curve of the three Se formes is Nano-Se>Se-Met>Na₂SeO₃. Foundation item: Supported by the Key Item of the Science and Technology Bureau of Zhejiang Province (021122680) Biography: Xu Bao-hua (1966-), male, Associate professor, Ph. D. research direction: nanobiology and animal nutrition.     

Laplace Transformation and Ergodic Potential Kernel for q-Process in Random Environment

This paper introduces some concepts such as q-process in random environment, Laplace transformation, ergodic potential kernel, error function and some basic lemmas.We study the continuity and Laplace transformation of random transition function. Finally, we give the sufficient condition for the existence of ergodic potential kernel for homogeneous q-processes in random environments.     

Convergence in the<Emphasis Type="Italic">r</Emphasis>-th mean and the Marcinkiewicz type weak law of large numbers for weighted sums of<Emphasis Type="Italic">L</Emphasis><Subscript>q</Subscript>-mixingale arrays

L _r convergence and convergence in probability for weighted sums ofL _q-mixingale arrays have been discussed and the Marcinkiewicz type weak law of large numbers forL _q-mixingale arrays has been obtained. Foundation item: Supported by the National Natural Science Foundation of China (10071058) Biography: Gan Shi-xin (1939-), male, Professor, research direction; martingale theory, probability limiting theory and Banach space geometry theory.     

On vector quasi-variational inequality problems in<Emphasis Type="Italic">H</Emphasis>-space

The purpose to this paper is to study the existence problem of solutions to the vector quasivariational inequality for vector-valued functions inH-space. Foundation item: Supported by the Science Research Foundation of Xianning Teacher’s College (No. K9911) Biography: Mao Jian-feng (1960-), male, Lecturer, research direction: nonlinear functional analysis.     

The Classificat,on of States for Markov Chain in Random Environments and m-irreducible

First of all, we introduces the concept of m-irreducible of Markov chain in random environment. Then under the condition of m-irreducible, the relationship of recurrent and positive recurrent between two states is studied. We also give several conditions that can imply a state is recurrent and positive recurrent. And then the period of a state is discussed and we obtained that under the condition of m-irreducible, for any two states in x, they have the same period.     

Proxy Multisignature Scheme with （t, m） Threshold Shared Verification

A proxy multisignature scheme with （t, m） threshold shared verification is first proposed based on elliptic curve. In proposed scheme, a proxy signer can sign a message on behalf of n original signers for specific group of verifiers and only any t or more verifiers together in the specific group of verifiers are able to verify the validity of the proxy multisignature on behalf of the group of specific verifiers, but t-1 or fewer verifiers can not. Since the proposed scheme is constructed based on elliptic curve, it requires smaller bandwidth and achieves more secrecy of data transmission; it is more etffcient for signature and verification etc.     

Strong Law of Large Numbers and Complete Convergence for Sequences of φ-Mixing Random Var, ables

We give some theorems of strong law of large numbers and complete convergence for sequences of φ-mixing random variables. In particular, Wittmann＇s strong law of large numbers and Teicher＇s strong law of large nnumbers for independent random variables are generalized to the case of φ -minxing random variables.     

Spectral Characteristics of the Best Affine Approach of Multi-Output m-Valued Logical Functions

This paper discusses the best affine approach （BAA） of multi-output m-valued logical functions. First, it gives the spectra of rate of accordance between multi-output m-valued logical functions and their affine functions, then analyzes the BAA of multi-output m-valued logical functions and finally gives the spectral characteristics of BAA of multi-output m-valued logical functions.     

Effect of UV-B Radiation on Ingesting and Nutritional Selecting Behavior of Rotifer Brachionus urceus

Effect of UV-B radiation on ingesting and nutritional selecting behavior of the roifer Brachionus urceus on 5 species of microlgae were studied under controlled laboratory conditions. Restdts showed that enhanced UV-B radiation significantly inhibited ingesting of the rotifer B. urceus when it was fed with 5 species of micro-algae （p〈0.05）. The ingesting selectivity rate varied with the UV-B radiation enhancement when it was fed with 5 species micro-algal mixture. Results indicated that the enhanced UV-B radiation could affect ingesting and nutritional selectivity of B. urceus.     

Biosorption of Cu（Ⅱ） and Pb（Ⅱ） by Auricularia polytricha

For searching biological material for heavy metal removal of waste-water, using macrofungus Auricularia polytricha as biosorbent for Cu^2＋ and Pb^2＋ removal was investigated. After shaking and biosorbing Cu^2＋ and Pb^2＋ in solution by biosorbents, the filtrates were tested by AAS and the adsorbed quantity of Cu^2＋ and Pb^2＋ was calculated. The biosorbents were effective in removal of Cu^2＋ and Pb^2＋ on the biosorbents that showed a high- est value around pH 5-6. The biosorption rate of Cu^2＋ and Pb^2＋ on A. polytricha biomass decreased with increasing the initial concentration of Cu^2＋ and Pb^2＋ in the medium. The biosorption of Cu^2＋ and Pb^2＋ on the biomasses follows pseudo-second order kinetics. The determined maximum biosorption capacities presented by the fungus biomass were 3.34 and 13.03 mg·g^-1 dry weight for Cu^2＋ and Pb^2＋, respectively by the biosorption equilibrium with Langmuir adsorption isotherm. According to the whole data analysis in each experiment of studying Cu^2＋ and Pb^2＋ biosorption including condition factors and adsorption isotherm, the adsorbed capacity of Pb^2＋ by A. polytricha biomass was bigger than Cu^2＋. The biosorption by A. polytricha was most effective when pH 5-6. The biosorbents are suitable for low Cu^2＋ and Pb^2＋ concentration waste-water, especially for Pb^2＋ removal.     

Quantitative Estimates of Outcrossing Rates in a Natural Population of Caldesia grandis （Alismataceae）

Outcrossing rate in a natural population of Caldesia grandis was estimated by the dominant random amplified polymorphism DNA (RAPD) marker using 10 open-pollinated progeny arrays of 24 individuals. The multilocus outcrossing rate estimated based on all 25 RAPD loci was 0.872 ±0.033 and the single-locus outcrossing rate was 0.795 ±0.032. Multilocus estimates did not differ significantly from the single-locus estimates. The fixation index, F, in the progeny estimated from RAPD data was -0.142 ±0.000. The estimates of multilocus outcrossing rates (t_m) and single-locus outcrossing rates (t_s) obtained from MLDT clearly indicate that outcrossing is predominant in the open-pollinated C. grandis population. An empirical analysis suggests that 15 should be the minimum number of dominant marker loci necessary to achieve robust estimates of t_m.     

Climate change over southern Qinghai Plateau in the past 500 years recorded in <Emphasis Type="Italic">Sabina tibetica</Emphasis> tree rings

Two tree ring-width chronologies of Sabina tibetica were developed based on cores sampled in Qumalai and Zhiduo, southern Qinghai Plateau. The response function analysis showed that the chronologies were sensitive to temperature and precipitation from April to June in the plateau. Moisture index (MI) was defined, reconstructed and extended back to AD 1550. The cross-validation method was used to check the stability of the calibration equation, and the result indicated that the equation was stable. Six severe dry periods were found in this region in the past 453-year reconstruction, which were 1592 to 1610, 1649 to 1665, 1687 to 1697, 1740 to 1750, 1818 to 1829 and 1918 to 1933. Five severe wetting periods were 1669 to 1682, 1700 to 1709, 1800 to 1814, 1898 to 1909 and 1935 to 1950. Spectrum analysis indicated that there existed long-term cycles of 60.4 and 53.4 a, solar cycle of 11 a and short-term cycles of 8, 6 and 4 a in the reconstructed series.     

On the Strong Law of Large Numbers for Non-Independent B-Valued Random Variables

This paper investigates some conditions which imply the strong laws of large numbers for Banach space valued random variable sequences. Some generalizations of the Marcinkiewicz- Zygmund theorem and the HoffmannJφrgensen and Pisier theorem are obtained.     

Research of Inclusion Complexation of 4,4＇-Thiodiphenol Effected by β-Cyclodextrin in Aqueous Solutions and the Effect on Photodegradation

The inclusion behavior of 4, 4＇ -Thiodiphenol （TDP）, a typical bisphenol and endocrine disruptor, reacts with β-cyclodextrin （β-CD） in aqueous solutions has been investigated by means of UV absorption spectrum and quantum-chemical calculation with Gaussian 98 software. The results show that the inclusion behavior of TDP is quite different in acidic solutions （pH 5.9） from that in alkaline solutions （pH 10.0）. This behavior difference is attributed to the different formula structures in aqueous solutions at acidic and alkaline pH values that are demonstrated by quantum- chemical modeling and calculation. TDP forms a 1 ： 1 fl-CD inclusion complex in aqueous solutions. The equiiibrium constant K was calculated to be 553.49 L/mol at pH 5.9 and 1 318.20 L/mol at pH 10.0 respectively for the inclusion complex reaction by using the modified Benesi-Heldbrand equation. After inclusion TDP＇s structure is changed especially at the inclusion part with the bond order becoming larger, which results in inhibitive photodegradation during direct photooxidation and H2O2 assisted photooxidation.     

Microstructure and Corrosion Resistance of Cr7C3/γ-Fe Ceramal Composite Coating Fabricated by Plasma Cladding

A new type in situ Cr7C3/γ-Fe ceramal composite coating was fabricated on substrate of hardened and tempered grade C steel by plasma cladding with Fe-Cr-C alloy powders. The ceramal composite coating has a rapidly solidified microstructure consisting of primary Cr7C3/γ- and the Cr7C3/γ-Fe eutectics, and is metallurgically bonded to the degree C steel substrate. The corrosion resistances of the coating in water solutions of 0.5 mol/L H2SO4 and 3.5% NaCl were evaluated utilizing the electrochemical polarization corrosion-test method. Because of the inherent excellent corrosion-resisting properties of the constituting phase and the rapidly solidified homogeneous microstructure, the plasma clad ceramal composite coating exhibits excellent corrosion resistance in the water solutions of 0.5 mol/L H2SO4 and 3.5% NaCl.     

A novel <Emphasis Type=Italic>in vitro</Emphasis> angiogenesis model based on a microfluidic device

Angiogenesis is very important for many physiological and pathological processes. However, the molecular mechanisms of angiogenesis are unclear. To elucidate the molecular mechanisms of angiogenesis and to develop treatments for angiogenesis-dependent diseases, it is essential to establish a suitable in vitro angiogenesis model. In this study, we created a novel in vitro angiogenesis model based on a microfluidic device. Our model provides an in vivo-like microenvironment for endothelial cells (ECs) cultures and monitors the response of ECs to changes in their microenvironment in real time. To evaluate the potential of this microfluidic device for researching angiogenesis, the effects of pro-angiogenic factors on ECs proliferation, migration and tube-like structure formation were investigated. Our results showed the proliferation rate of ECs in 3D matrix was significantly promoted by the pro-angiogenic factors (with an increase of 59.12%). With the stimulation of pro-angiogenic factors gradients, ECs directionally migrated into the Matrigel from low concentrations to high concentrations and consequently formed multi-cell chords and tube-like structures. These results suggest that the device can provide a suitable platform for elucidating the mechanisms of angiogenesis and for screening pro-angiogenic or anti-angiogenic drugs for angiogenesis-dependent diseases.