高效液相色谱法测定菊粉酶解产物

采用高效液相色谱法以Shim-pack CLC-NH₂ 柱为分析柱，乙腈与水(体积比为7∶3)为流动相，以RID-6A示差折光仪为检测器，测定了菊粉酶解产物中的葡萄糖、果糖和蔗糖 ,为菊粉酶解产物的测定提供了科学依据。     

固定化条件对葡萄糖酶电极响应性能的影响

用微铂盘电极和交联法制得的酶膜构成葡萄糖酶电极，测定其电流响应特性，研究了酶的固定化条件：酶含量以及载体蛋白和交联剂的用量等，对响应特性的影响。提出较适宜的酶电极制备方法，并检测了所得的电极在不同ｐＨ溶液中的灵敏度，指出其最佳操作条件。     

阳离子交换固相萃取在三聚氰胺检测的应用

采用阳离子交换固相萃取小柱分离富集液态奶中三聚氰胺，用甲醇洗脱后，以C8反相柱离子对液相色谱分析测试三聚氰胺含量．实验中考察了操作条件对液态奶中三聚氰胺测定结果的影响，对固相萃取前处理条件进行了优化，结果表明，固相萃取前处理过程的最佳洗脱体积8mL，流速0．5mL／min．工作曲线方程为Y=4．0963x＋21．508，相关系数为R^2=0．9978，相对标准偏差小于3．1％，回收率在96．4％-102．9％之间，结果令人满意．     

高效液相色谱法检测蔗渣半纤维素水解物中的单糖

用高效液相色谱法测定甘蔗渣半纤维素水解物中的单糖含量。以Ｈｙｐｅｒｓｉｌ ＮＨ２柱为色谱分析柱，用示差折光检测器检测，流动相是乙腈：水（８０：２０）。在此色谱条件下，甘蔗渣半纤维素水解物分离成：木糖、阿拉伯糖、果糖和葡萄糖。各单糖呈良好的线性。加样回收率的平均值为９７．３％～９８．７％，此方法简便、快速、准确。     

抗干扰微型葡萄糖酶电极的研制

铂丝电极(长0．8cm，直径100μm)经铂化处理后，将3位取代的吡咯衍生物(3-羰基丁酸吡咯)与单体吡咯以适当的比例混合，与葡萄糖氧化酶(GOD)一起用循环伏安法电聚合到铂化的铂丝电极上，形成杂聚吡咯-GOD膜，制备成葡萄糖微酶电极。该微酶电极灵敏度高(每毫摩尔葡萄糖电流响应可以达到700nA)，响应范围大(0．1～110mmol／L)，抗干扰(抗坏血酸在该电极上的响应电流不到对比电极(无膜)的1．0％)，电极的稳定性良好，间断测定40d，灵敏度变化不大。     

模拟过氧化物酶用于葡萄糖的测定方法研究

本文研究了利用模拟过氧化物酶(Mn-TPPS_4)测定葡萄糖的反应条件,并采用流动注射的办法对血清中葡萄糖的含量进行了测定,结果令人满意。此方法快速,简便,测定葡萄糖的检测限为2.7×10~(-9)mol/l。     

蔗糖酶促水解液相色谱分离耦合过程

在φ１０ｍｍ×１５００ｍｍ色谱柱上，同时进行蔗糖酶促水解与液相色谱分离，制备葡萄糖和果糖．Ｃａ２＋型００３×７离子交换树脂为固定相，稀酶溶液为流动相，系统研究了酶浓度、流速、进料体积、进料浓度对过程的影响，建立了描述过程的数学模型并进行了数值求解．结果表明，采用该耦合方式不仅显著加快水解速率，而且可直接收集到富葡萄糖浆与富果糖浆，减少了下游进一步分离操作的强度与费用．理论计算值与实验值在低浓度下吻合良好。     

氯霉素分子烙印固相萃取柱的制备及萃取条件优化

采用分子烙印技术制备了氯霉素的分子烙印固相萃取柱,并通过对其吸附能力、解吸条件等特性的研究,确定了分子烙印固相萃取柱的上柱、淋洗和洗脱条件．结果表明,与目前氯霉素检测中所采用的传统C18柱相比,分子烙印固相萃取柱可采用甲醇含量较高的甲醇／水混合溶剂作为淋洗溶剂,用乙腈含量为40％(体积分数)的乙腈／水作为洗脱溶剂,且比传统C18柱具有更大的柱容量．分子烙印固相萃取柱的优点使其可应用于实际生物样品中氯霉素的检测,以解决目前氯霉素残留检测中面临的难题．     

纳米ZnO固载辣根过氧化物酶及其降解酚类化合物研究

目的 利用纳米材料固载辣根过氧化物酶并对固载酶性质及应用进行研究.方法 运用电镜,红外光谱法表征材料,用紫外光谱法检测固载化HRP活性.结果 固载酶在碱性环境中活性比游离酶平均高40%,60℃反应体系里仍然能维持45.3%活性,保存50 d后活性为35.8%,用于降解苯酚与氯酚时去除效率分别为87.3%和65.4%.结论 实验证明了纳米ZnO能够固载辣根过氧化物酶,所得固载酶应用于降解苯酚、氯酚水溶液时催化效率比游离酶分别提高39.68%和21.56%.但是,固载化HRP的重复利用性较差,4次循环使用后基本失活.有必要进一步探索优化固载与催化条件,提高固载酶重复利用性.     

葡萄糖酶电极在酶法生产葡萄糖工业中的应用

本实验用葡萄糖酶电极测定了淀粉糖化过程中葡萄糖含量的变化，该方法快速，准确，对酶法淀粉制糖工业具有重要的意义。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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