Internalization of triiodothyronine-bovine serum albumin-colloidal gold complexes in human peripheral leukocytes

Summary Complexes of triiodothyronine-bovine serum albumin-colloidal gold were specifically internalized in human peripheral leukocytes after 5 min of incubation. The specifity was partially lost after a longer incubation.     

Mitogenic activity of selenoorganic compounds in human peripheral blood leukocytes

A variety of organoselenium compounds were originally described as antiinflammatory, antioxidant or glutathione-peroxidase-like agents, and as inhibitors of prostaglandin and leukotriene synthesis. Recently, the compounds have also been found to be inducers of interferon gamma and tumor necrosis factor in human peripheral blood leukocytes (PBL). We evaluated the effects of bis [2-(N-phenylcarboxamido)phenyl] diselenide and Ebselen; 2-phenyl-1,2-benzisoselenazol-3(2H)one, on the incorporation of tritiated thymidine into the DNA of PBL cultured in vitro. Both compounds were mitogenic and this effect was correlated with the expression of interleukin 2 receptor in T-lymphocytes. Therefore, we suggest that the selenoorganic compounds may induce mitogenic cytokines.     

Mitogenic activity of selenoorganic compounds in human peripheral blood leukocytes

Summary A variety of organoselenium compounds were originally described as antiinflammatory, antioxidant or glutathione-peroxidase-like agents, and as inhibitors of prostaglandin and leukotriene synthesis. Recently, the compounds have also been found to be inducers of interferon gamma and tumor necrosis factor in human peripheral blood leukocytes (PBL). We evaluated the effects of bis [2-(N-phenylcarboxamido)phenyl] diselenide and Ebselen^®; 2-phenyl-1,2-benzisoselenazol-3(2H)one, on the incorporation of tritiated thymidine into the DNA of PBL cultured in vitro. Both compounds were mitogenic and this effect was correlated with the expression of interleukin 2 receptor in T-lymphocytes. Therefore, we suggest that the selenoorganic compounds may induce mitogenic cytokines.     

Binding of (125I) triiodothyronine to human peripheral leukocytes and its internalization

Ultrastructural autoradiography showed high specific binding of (125I) triiodothyronine, as confirmed by a competition test, to plasma membranes, nuclei and mitochondria of human peripheral leukocytes. A high level of binding was also noted on the granulocytes' granules, especially in eosinophils.     

Binding of (125I) triiodothyronine to human peripheral leukocytes and its internalization

Summary Ultrastructural autoradiography showed high specific binding of (¹²⁵I) triiodothyronine, as confirmed by a competition test, to plasma membranes, nuclei and mitochondria of human peripheral leukocytes. A high level of binding was also noted on the granulocytes' granules, especially in eosinophils.     

Organoselenides as potential immunostimulants and inducers of interferon gamma and other cytokines in human peripheral blood leukocytes

Summary A number of organoselenium compounds have been described as anti-inflammatory, antioxidant, glutathione peroxidase-like agents and inhibitors of prostaglandin synthesis. Here we report that bis [2-(N-phenyl-carboxamido)]phenyl diselenide, 2-phenyl-1,2-benzisoselenazol-3(2H)-one (Ebselen) and related compounds are inducers of interferon gamma (IFN-) and tumor necrosis factor (TNF) in human peripheral blood leukocytes. The IFN and TNF response was rapid, occurring within 20 h, and high-up to 1000 and 2000 units ml^–1-and was clearly related to the dosage and the structure of the compounds. The action of the compounds and phytohemagglutinin was synergistic. The IFN gamma and TNF production was reduced after removing adherent cells. Although the mode of action of the compounds is not known, they appear to interact directly or indirectly with both adherent and non-adherent leukocytes, and stimulate the synthesis of a set of different cytokines including factors controlling the cell proliferation. Therefore, organoselenides may be regarded as the biological response modifiers.     

Cholesterol synthesis in freshly isolated human leukocytes

Summary Cholesterol synthesis has been studied in human leukocytes shortly after the isolation from healthy subjects. Not delipidated human serum reduced the cholesterol synthesis when added to the incubation medium. A similar effect was obtained when the leukocytes were incubated in the presence of physiological concentrations of low density lipoproteins.     

Differences between plasma and serum mediated chemotaxis of leukocytes

Zusammenfassung Die chemotaktische Aktivität von Kaninchenplasma und -serum für neutrophile Leukozyten und Makrophagen ist verschieden. Normales Serum enthält im Gegensatz zum Plasma chemotaktische Faktoren für Neutrophile und Makrophagen. Inkubation von Serum mit einem Immunkomplex bewirkt einen erheblichen Anstieg der chemotaktischen Aktivität für Neutrophile, dagegen eine Verminderung für Makrophagen. Die Inkubation von Plasma mit Immunkomplex bewirkt einen starken Anstieg für beide Zelltypen. Nach Inkubation einer Granula-Fraktion aus Neutrophilen mit Plasma — nicht aber mit Serum — entstellt chemotaktische Aktivität für Makrophagen. Diese Befunde lassen einen möglichen Zusammenhang zwischen Chemotaxis und Koagulation vermuten.     

Cholesterol synthesis in freshly isolated human leukocytes.

Cholesterol synthesis has been studied in human leukocytes shortly after the isolation from healthy subjects. Not delipidated human serum reduced the cholesterol synthesis when added to the incubation medium. A similar effect was obtained when the leukocytes were incubated in the presence of physiological concentrations of low density lipoproteins.     

Different methotrexate effects in cultured normal and leukaemic human leukocytes

Summary Evidence is presented that the use of 2 different culture media, with or without methionine, can help to distinguish between normal and leukaemic methotrexate (MTX)-sensitive cells derived from chronic myeloid leukaemia patients. During the blast crisis MTX assays may help directly in the diagnostics of lymphoid involvement.This work was supported by the Polish Academy of Sciences within the project 09.7.4.1.4.Acknowledgments. We are indebted to Prof. Zofia Zieliska, Dept. of Cell Biochemistry, M. Nencki Institute of Experimental Biology PAN for providing the 5-methyltetrahydrofolate and for advice in these studies.     

In vitro response of human leukocytes to anti-human-thymocyte globulin

Résumé La faculté qu'ont le sérum (ALS) antithymocyte du cheval et son dérivé IgG (ALG) de stimuler la synthèse du DNA avec les leukocytes péripheriques humains a été contrôlée in vitro. Sauf déviation même faible de dosage, l'ALS et l'ALG stimulent la synthèse du DNA. Une forte concentration de ALG inhibe l'effet stimulant de la phytohémagglutinine. Cela peut contribuer en partie à la mort de la cellule ou à sa dégradation à cause de la grande concentration de l'ALS ou de l'ALG.     

Morphology of colloidal gold, ferritin and anti-ferritin antibody complexes.

The morphology of model complexes between colloidal gold, ferritin and anti-ferritin antibodies has been studied in order to evaluate the potential of colloidal gold as a cytochemical marker.     

The demonstration of D (Rh) antigen in human leukocytes

Zusammenfassung Mit indirekter Fluoreszenz-Antik?rper-Methode wurde nachgewiesen, dass Leukozyten Rh-positiver Versuchspersonen D-Antigen enthalten.