Incorporation of phospholipids in Ehrlich ascites tumor cells

Ehrlich ascites tumor cells (EATC) were incubated with unilamellar vesicles (UV) or multilamellar vesicles (MV). UV and MV were incorporated differently into EATC. The increase in 32P-phospholipid in EATC in the presence of UV was 12% in 300 min. Absorption of phospholipid from MV could account for only 3%. About 50% of the UV incorporation of 32P was by endocytosis and/or fusion.     

Heterophile antigens in Ehrlich ascites tumor cells

Riassunto Gli autori hanno osservato che i sieri di animali immunizzati con cellule del tumore cancro ascite di Ehrlich sono emolitici per gli eritrociti di topo, ratto e montone e presumono che le cellule del tumore di Ehrlich possano contenere frazioni antigene di tipo eterogenetico.     

Interaction between mycoplasmas and Ehrlich ascites tumor cells

Zusammenfassung Es wurde gezeigt, dass die Adsorption von Mycoplasmen an Ehrlich Ascites-Tumorzellen an das Vorhandensein von neuraminidase-empfindlichen Zellrezeptoren gebunden ist.     

Glycerin prevents the 'heat-death' of Ehrlich ascites tumor cells

When heated to 45 degrees C for 20 min Ehrlich ascites tumor and NK-lymphoma cells are irreversibly damaged, so that they lose transplantability. The presence of 7-8% glycerol during heat treatment protects EAT cells completely and NK-lymphoma cells partly against this injury.     

Effect of tannic acid on the Ehrlich ascites tumor cells

Summary Tannic acid was used to fix and stain Ehrlich ascites tumor cells in suspension. An increase in surface microvilli and cytoplasmic blebs in the tumor cells was observed. The mechanism of tannic acid induced surface morphological changes in tumor cells and the formation of a precipitate of protein-tannic acid-heavy metal complex are discussed.Acknowledgment. The author is grateful to Professor F. P. Lisowski for his advice and helpful criticism and to Mr Y. S. Tong for his expert technical assistance. This project was supported by a grant from the Hong Kong University Research Grants Committee.     

Relationship between A-type and C-type particles in Ehrlich ascites tumor cells

Summary Intracisternal and intracytoplasmic A-type particles were discovered in Ehrlich ascites tumor cells. In addition, mature and immature C-type particles were also seen in the intercellular space. It is believed that A particles may represent a precursor or a formative stage of the C particles.Acknowledgment. The author is grateful to Professor F. P. Lisowski for his advice and helpful criticisms and to Mr Y. S. Tong for his expert technical assistance. This project was supported by a grant from the Hong Kong University Research Grants Committee.     

Effect of tannic acid on the Ehrlich ascites tumor cells.

Tannic acid was used to fix and stain Ehrlich ascites tumor cells in suspension. An increase in surface microvilli and cytoplasmic blebs in the tumor cells was observed. The mechanism of tannic acid induced surface morphological changes in tumor cells and the formation of a precipitate of protein-tannic acid-heavy metal complex are discussed.     

Relationship between A-type and C-type particles in Ehrlich ascites tumor cells.

Intracisternal and intracytoplasmic A-type particles were discovered in Ehrlich ascites tumor cells. In addition, 'mature' and 'immature' C-type particles were also seen in the intercellular space. It is believed that A particles may represent a precursor or a formative stage of the C particles.     

Stimulation of hemin synthesis in Ehrlich ascites tumor cells by mouse liver RNA

Résumé Les cellules ascites de la tumeur Ehrlich couvaient in vitro avec le précurseur radioactif de l'hémine-aminolévulinic acid-4-C¹⁴. Les préparations de ARN du foie de la souris stimulaient le niveau de synthèse de l'hémine.     

Physical properties of deoxyribonucleic acid isolated from Ehrlich ascites tumor cells by various methods

Zusammenfassung Die Desoxyribonukleinsäure des Ehrlich-Karzinoms wurde nach vier verschiedenen Methoden isoliert und Stickstoff- und Phosphorgehalt sowie die physikalischen Eigenschaften für jedes der vier Präparate bestimmt. Die mit Hilfe von Natrium-Xylolsulfonat isolierte Desoxyribonukleinsäure scheint am stärksten polymerisiert zu sein.     

Inhibition of granulocyte-macrophage (GM) colony formation by basic polypeptides from mouse Ehrlich ascites tumor cells

Summary The polypeptides isolated from mouse Ehrlich ascites tumor cells were tested for their inhibitory activity against GM colony formation. It was found that basic polypeptides with low molecular weight evidently inhibit colony formation. Our data reveal that the tested polypeptides may show chalone activity.